TBLASTN 2.6.0+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: genome.fa
           3,184 sequences; 1,209,001,349 total letters



Query= SPP00000065_2.0 # Protein # Selenocysteine synthase (SecS) # Human

Length=501
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

PTFA01000163.1  Casuarius casuarius isolate B32419 scaffold_162, ...  174     6e-45


>PTFA01000163.1 Casuarius casuarius isolate B32419 scaffold_162, whole genome 
shotgun sequence
Length=2401681

 Score = 174 bits (441),  Expect = 6e-45, Method: Compositional matrix adjust.
 Identities = 89/124 (72%), Positives = 92/124 (74%), Gaps = 29/124 (23%)
 Frame = +1

Query  89      YRFIHGIGRSGDISAVQPKAAGSSLLNKITNSLVLDIIKLAG------------------  130
               +R IHGIGRSGDISAVQPKAAGSSLLNK+TNS+VLDIIKLAG                  
Sbjct  588748  FRLIHGIGRSGDISAVQPKAAGSSLLNKLTNSVVLDIIKLAGRCKAKSISLLCGHVMCSS  588927

Query  131     -----------VHTVANCFVVPMATGMSLTLCFLTLRHKRPKAKYIIWPRIDQKSCFKSM  179
                          V TV NCFVVPMATGMSLTLCFLTLRHKRPKAKYIIWPRIDQKSCFKSM
Sbjct  588928  C*FLTCFLCAGVRTVTNCFVVPMATGMSLTLCFLTLRHKRPKAKYIIWPRIDQKSCFKSM  589107

Query  180     ITAG  183
               ITAG
Sbjct  589108  ITAG  589119


 Score = 91.3 bits (225),  Expect(2) = 2e-30, Method: Compositional matrix adjust.
 Identities = 41/51 (80%), Positives = 48/51 (94%), Gaps = 0/51 (0%)
 Frame = +2

Query  263     MHLIQQGARVGRIDAFVQSLDKNFMVPVGGAIIAGFNDSFIQEISKMYPGR  313
               + ++ +G RVGRIDAFVQSLDKNFMVPVGGAIIAGF++SFIQEISKMYPG+
Sbjct  597986  VFVLVKGTRVGRIDAFVQSLDKNFMVPVGGAIIAGFSESFIQEISKMYPGK  598138


 Score = 65.5 bits (158),  Expect(2) = 2e-30, Method: Compositional matrix adjust.
 Identities = 31/50 (62%), Positives = 42/50 (84%), Gaps = 0/50 (0%)
 Frame = +3

Query  306     ISKMYPGRASASPSLDVLITLLSLGSNGYKKLLKERKEMFSYLSNQIKKL  355
               +S ++ GRASASPSLDVLITLLSLG++GYK+LLKERK   ++L + + K+
Sbjct  598239  MSAVFSGRASASPSLDVLITLLSLGTSGYKQLLKERKVSSTFLKSSVNKV  598388


 Score = 116 bits (291),  Expect = 1e-25, Method: Compositional matrix adjust.
 Identities = 52/62 (84%), Positives = 57/62 (92%), Gaps = 0/62 (0%)
 Frame = +2

Query  30      HLIRLLLEKGKCPENGWDESTLELFLHELAIMDSNNFLGNCGVGEREGRVASALVARRHY  89
               ++I  L  KGKCPE+GWDEST+ELFLHELAIMDSNNFLGNCGVGEREGRVAS LVARRHY
Sbjct  586283  YIIIFLFIKGKCPEDGWDESTIELFLHELAIMDSNNFLGNCGVGEREGRVASGLVARRHY  586462

Query  90      RF  91
               R+
Sbjct  586463  RY  586468


 Score = 112 bits (279),  Expect = 3e-24, Method: Compositional matrix adjust.
 Identities = 49/65 (75%), Positives = 57/65 (88%), Gaps = 0/65 (0%)
 Frame = +2

Query  404     RVVPLGSMQTVSGYTFRGFMSHTNNYPCAYLNAASAIGMKMQDVDLFIKRLDRCLKAVRK  463
               RVVP GS+QTV+ YTF+GFMSHTN+YPCAYLNAASAIG+K QDVD+F+KRLD+CLK  RK
Sbjct  608693  RVVPCGSVQTVNNYTFKGFMSHTNDYPCAYLNAASAIGIKKQDVDVFLKRLDKCLKTSRK  608872

Query  464     ERSKE  468
               E   E
Sbjct  608873  EVKNE  608887


 Score = 95.1 bits (235),  Expect = 1e-18, Method: Compositional matrix adjust.
 Identities = 43/52 (83%), Positives = 48/52 (92%), Gaps = 0/52 (0%)
 Frame = +2

Query  183     GFEPVVIENVLEGDELRTDLKAVEAKVQELGPDCILCIHSTTSCFAPRVPDR  234
               GFEPVVIENVLEGDELRTD+KAVEAK++ LG + ILC+HSTTSCFAPR PDR
Sbjct  591959  GFEPVVIENVLEGDELRTDIKAVEAKIRALGAENILCVHSTTSCFAPRAPDR  592114


 Score = 77.0 bits (188),  Expect = 4e-13, Method: Compositional matrix adjust.
 Identities = 35/35 (100%), Positives = 35/35 (100%), Gaps = 0/35 (0%)
 Frame = +3

Query  234     RLEELAVICANYDIPHIVNNAYGVQSSKCMHLIQQ  268
               RLEELAVICANYDIPHIVNNAYGVQSSKCMHLIQQ
Sbjct  595857  RLEELAVICANYDIPHIVNNAYGVQSSKCMHLIQQ  595961


 Score = 56.6 bits (135),  Expect = 1e-06, Method: Compositional matrix adjust.
 Identities = 26/34 (76%), Positives = 30/34 (88%), Gaps = 0/34 (0%)
 Frame = +1

Query  371     ISLAMTLKTLDEHRDKAVTQLGSMLFTRQVSGAR  404
               ++ AM+LK LDE+ D AVTQLGSMLFTRQVSGAR
Sbjct  606463  VTSAMSLKNLDENNDAAVTQLGSMLFTRQVSGAR  606564


 Score = 53.5 bits (127),  Expect = 9e-06, Method: Compositional matrix adjust.
 Identities = 23/33 (70%), Positives = 30/33 (91%), Gaps = 0/33 (0%)
 Frame = +3

Query  342     KEMFSYLSNQIKKLSEAYNERLLHTPHNPISLA  374
               +EMFSYLS+++KKL++ +NERLL TPHNPISL 
Sbjct  605724  QEMFSYLSSELKKLADVHNERLLDTPHNPISLG  605822


 Score = 45.1 bits (105),  Expect = 0.004, Method: Compositional matrix adjust.
 Identities = 21/38 (55%), Positives = 27/38 (71%), Gaps = 0/38 (0%)
 Frame = +1

Query  1       MNRESFAAGERLVSPAYVRQGCEARRSHEHLIRLLLEK  38
               MN E+F   ERLV+  YVRQG + RR+HE  +R LLE+
Sbjct  584857  MNAENFGCSERLVTAGYVRQGAQGRRAHELRLRALLEQ  584970



Lambda      K        H        a         alpha
   0.320    0.135    0.394    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 149359602259


  Database: genome.fa
    Posted date:  Oct 19, 2018  9:06 AM
  Number of letters in database: 1,209,001,349
  Number of sequences in database:  3,184



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40
