TBLASTN 2.6.0+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: genome.fa
           3,184 sequences; 1,209,001,349 total letters



Query= SPP00000146_2.0 # Protein # tRNA Sec 1 associated protein 1 (SECp43)
# Human

Length=287
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

PTFA01000044.1  Casuarius casuarius isolate B32419 scaffold_43, w...  94.4    7e-20
PTFA01000235.1  Casuarius casuarius isolate B32419 scaffold_234, ...  68.2    5e-11
PTFA01000493.1  Casuarius casuarius isolate B32419 scaffold_492, ...  58.5    7e-08
PTFA01001586.1  Casuarius casuarius isolate B32419 scaffold_1585,...  48.9    8e-05
PTFA01000047.1  Casuarius casuarius isolate B32419 scaffold_46, w...  38.9    0.13 
PTFA01000232.1  Casuarius casuarius isolate B32419 scaffold_231, ...  38.5    0.16 
PTFA01000081.1  Casuarius casuarius isolate B32419 scaffold_80, w...  37.7    0.27 
PTFA01000371.1  Casuarius casuarius isolate B32419 scaffold_370, ...  37.7    0.33 
PTFA01000093.1  Casuarius casuarius isolate B32419 scaffold_92, w...  37.0    0.57 
PTFA01000051.1  Casuarius casuarius isolate B32419 scaffold_50, w...  33.9    4.4  
PTFA01000071.1  Casuarius casuarius isolate B32419 scaffold_70, w...  33.5    6.8  


>PTFA01000044.1 Casuarius casuarius isolate B32419 scaffold_43, whole genome 
shotgun sequence
Length=5744358

 Score = 94.4 bits (233),  Expect = 7e-20, Method: Compositional matrix adjust.
 Identities = 47/56 (84%), Positives = 48/56 (86%), Gaps = 3/56 (5%)
 Frame = -1

Query  93       SPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQTGVSK---GYGFVKFT  145
                SPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQ GVSK    Y F+ F 
Sbjct  5040615  SPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQAGVSK*VIFYAFLSFI  5040448


 Score = 89.4 bits (220),  Expect = 4e-18, Method: Compositional matrix adjust.
 Identities = 40/45 (89%), Positives = 43/45 (96%), Gaps = 0/45 (0%)
 Frame = -3

Query  243      DPMPQLDVTEANKEFMEQSEELYDALMDCHWQPLDTVSSEIPAMM  287
                DP PQLDV EANK+FMEQSEELYDALMDCHWQPLDTVSSEIPA++
Sbjct  5033437  DPTPQLDVHEANKQFMEQSEELYDALMDCHWQPLDTVSSEIPAVL  5033303


 Score = 86.7 bits (213),  Expect = 3e-17, Method: Compositional matrix adjust.
 Identities = 42/51 (82%), Positives = 47/51 (92%), Gaps = 3/51 (6%)
 Frame = -1

Query  127      KVVLDQTGVSKGYGFVKFTDELEQKRALTECQGAVGLGSKPVRLSVAIPKA  177
                +++LD   + +GYGFVKFTDELEQKRALTECQGAVGLGSKPVRLSVAIPKA
Sbjct  5037552  QIILD---LCRGYGFVKFTDELEQKRALTECQGAVGLGSKPVRLSVAIPKA  5037409


 Score = 75.5 bits (184),  Expect = 2e-13, Method: Compositional matrix adjust.
 Identities = 33/36 (92%), Positives = 34/36 (94%), Gaps = 0/36 (0%)
 Frame = -2

Query  43       IPAGYCFVEFADLATAEKCLHKINGKPLPGATPAKR  78
                IPAGYCFVEFADLATAEKCLHKINGKPLPGATP  +
Sbjct  5045150  IPAGYCFVEFADLATAEKCLHKINGKPLPGATPVSK  5045043


 Score = 65.1 bits (157),  Expect = 4e-10, Method: Compositional matrix adjust.
 Identities = 29/32 (91%), Positives = 31/32 (97%), Gaps = 0/32 (0%)
 Frame = -3

Query  11       EPYMDENFISRAFATMGETVMSVKIIRNRLTG  42
                EPYMDENF+SRAFATMGE V+SVKIIRNRLTG
Sbjct  5046022  EPYMDENFVSRAFATMGELVLSVKIIRNRLTG  5045927


 Score = 40.0 bits (92),  Expect = 0.056, Method: Compositional matrix adjust.
 Identities = 21/60 (35%), Positives = 34/60 (57%), Gaps = 1/60 (2%)
 Frame = -3

Query  3        ASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCL  62
                ASL++GDL P + E  +   F+  G  V+S+++ R+ +T    GY +V F   A  + CL
Sbjct  3220816  ASLYVGDLHPDVTEAMLYEKFSPAGP-VLSIRVCRDMITRRSLGYAYVNFQQPADGK*CL  3220640


 Score = 39.7 bits (91),  Expect = 0.081, Method: Compositional matrix adjust.
 Identities = 17/17 (100%), Positives = 17/17 (100%), Gaps = 0/17 (0%)
 Frame = -2

Query  76       AKRFKLNYATYGKQPDN  92
                AKRFKLNYATYGKQPDN
Sbjct  5042195  AKRFKLNYATYGKQPDN  5042145


 Score = 38.1 bits (87),  Expect = 0.23, Method: Compositional matrix adjust.
 Identities = 21/51 (41%), Positives = 33/51 (65%), Gaps = 1/51 (2%)
 Frame = -3

Query  129      VLDQTGVSKGYGFVKFTDELEQKRALTECQGAVGLGSKPVRLSVAIPKASR  179
                V+ + G SKG+GFV F+   E  +A+TE  G + +GSKP+ +++A  K  R
Sbjct  3211033  VMLEDGRSKGFGFVCFSSPEEATKAVTEMNGRI-VGSKPLYVALAQRKEER  3210884


 Score = 37.0 bits (84),  Expect = 0.54, Method: Compositional matrix adjust.
 Identities = 25/60 (42%), Positives = 32/60 (53%), Gaps = 4/60 (7%)
 Frame = -3

Query  94       PEYSLFVGDLTPDVDDGMLYEFFVKVYP--SCRGGKVVLDQTGVSKGYGFVKFTDELEQK  151
                P  SL+VGDL PDV + MLYE F    P  S R  + ++  T  S GY +V F    + K
Sbjct  3220822  PMASLYVGDLHPDVTEAMLYEKFSPAGPVLSIRVCRDMI--TRRSLGYAYVNFQQPADGK  3220649


>PTFA01000235.1 Casuarius casuarius isolate B32419 scaffold_234, whole genome 
shotgun sequence
Length=1545499

 Score = 68.2 bits (165),  Expect = 5e-11, Method: Compositional matrix adjust.
 Identities = 28/42 (67%), Positives = 35/42 (83%), Gaps = 0/42 (0%)
 Frame = +3

Query  243      DPMPQLDVTEANKEFMEQSEELYDALMDCHWQPLDTVSSEIP  284
                DP   LD+ E N++FME SEE YD+LM+CHWQPLDTV+S+IP
Sbjct  1506006  DPQLHLDIDEMNRQFMETSEEFYDSLMNCHWQPLDTVTSDIP  1506131


 Score = 55.5 bits (132),  Expect = 7e-07, Method: Compositional matrix adjust.
 Identities = 24/44 (55%), Positives = 33/44 (75%), Gaps = 0/44 (0%)
 Frame = +2

Query  94       PEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQTGVSK  137
                P+YS+FVG+LTP+VDD  LY++F+K YPSC   K+  D  G S+
Sbjct  1501217  PDYSIFVGELTPEVDDFQLYDYFLKRYPSCIDCKIATDLLGYSR  1501348


 Score = 47.8 bits (112),  Expect = 2e-04, Method: Compositional matrix adjust.
 Identities = 20/40 (50%), Positives = 29/40 (73%), Gaps = 0/40 (0%)
 Frame = +1

Query  137      KGYGFVKFTDELEQKRALTECQGAVGLGSKPVRLSVAIPK  176
                +GY +V+F ++ +Q RAL +CQ A GLG K +RLS+ I K
Sbjct  1502218  RGYAYVRFGEQGDQMRALQDCQNAPGLGGKRIRLSIGISK  1502337


>PTFA01000493.1 Casuarius casuarius isolate B32419 scaffold_492, whole genome 
shotgun sequence
Length=282151

 Score = 58.5 bits (140),  Expect = 7e-08, Method: Compositional matrix adjust.
 Identities = 47/150 (31%), Positives = 74/150 (49%), Gaps = 19/150 (13%)
 Frame = -2

Query  37     RNRLTGIPAGYCFVEF-----ADLATAEKCLHKINGKPLPGATPAKRFKLNYAT-YGKQP  90
              R  L G+ AGY FVEF     AD A     + K+ GKP+         ++N A+ + K  
Sbjct  80001  RKALWGLVAGYGFVEFLSEEDADYAIKIMNMIKLYGKPI---------RVNKASAHNKNL  79849

Query  91     DNSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLD-QTGVSKGYGFVKFTDELE  149
              D     ++F+G+L P++D+ +LY+ F       +  K++ D  TG SKGY F+ F    +
Sbjct  79848  DVGA--NIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFA-SFD  79678

Query  150    QKRALTECQGAVGLGSKPVRLSVAIPKASR  179
                 A  E      L ++P+ +S A  K S+
Sbjct  79677  ASDAAIEAMNGQYLCNRPITVSYAFKKDSK  79588


 Score = 48.5 bits (114),  Expect = 1e-04, Method: Compositional matrix adjust.
 Identities = 21/70 (30%), Positives = 41/70 (59%), Gaps = 0/70 (0%)
 Frame = -2

Query  1      MAASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEK  60
              + A++++G+L+P +DE  +   F+  G  + + KI+R+  TG   GY F+ FA    ++ 
Sbjct  79845  VGANIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFASFDASDA  79666

Query  61     CLHKINGKPL  70
               +  +NG+ L
Sbjct  79665  AIEAMNGQYL  79636


>PTFA01001586.1 Casuarius casuarius isolate B32419 scaffold_1585, whole genome 
shotgun sequence
Length=9197

 Score = 48.9 bits (115),  Expect = 8e-05, Method: Compositional matrix adjust.
 Identities = 28/67 (42%), Positives = 40/67 (60%), Gaps = 2/67 (3%)
 Frame = +3

Query  95    EYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLD-QTGVSKGYGFVKFTDELEQKRA  153
             ++ +F GDL  +V+D +L   F + YPS    KV+ D +TG +KGYGFV F D  +  RA
Sbjct  2028  DFRIFCGDLGNEVNDDILARAFGR-YPSFLKAKVIRDKRTGKTKGYGFVSFKDPNDYVRA  2204

Query  154   LTECQGA  160
             + E  G 
Sbjct  2205  MREMNGG  2225


>PTFA01000047.1 Casuarius casuarius isolate B32419 scaffold_46, whole genome 
shotgun sequence
Length=5675994

 Score = 38.9 bits (89),  Expect = 0.13, Method: Compositional matrix adjust.
 Identities = 35/90 (39%), Positives = 43/90 (48%), Gaps = 14/90 (16%)
 Frame = -2

Query  87       GKQPDNSPEY---SLFVGDLTPDVDDGMLYEFFVKVYP--SCRGGKVVLDQ-TGVSKGYG  140
                G+   ++P Y   SL+VGDL PDV + MLYE F    P  S R   V  D  T  S GY 
Sbjct  4736153  GRMNPSAPSYPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIR---VCRDMITRRSLGYA  4735983

Query  141      FVKFTDELEQKRALTECQGAVGLGSKPVRL  170
                +V F     Q+ A  E    VG G    R+
Sbjct  4735982  YVNF-----QQPADGEWSAGVGPGRAAARV  4735908


 Score = 37.0 bits (84),  Expect = 0.56, Method: Compositional matrix adjust.
 Identities = 19/57 (33%), Positives = 32/57 (56%), Gaps = 1/57 (2%)
 Frame = -2

Query  3        ASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAE  59
                ASL++GDL P + E  +   F+  G  ++S+++ R+ +T    GY +V F   A  E
Sbjct  4736117  ASLYVGDLHPDVTEAMLYEKFSPAG-PILSIRVCRDMITRRSLGYAYVNFQQPADGE  4735950


 Score = 35.4 bits (80),  Expect = 1.7, Method: Compositional matrix adjust.
 Identities = 19/51 (37%), Positives = 32/51 (63%), Gaps = 1/51 (2%)
 Frame = -1

Query  129      VLDQTGVSKGYGFVKFTDELEQKRALTECQGAVGLGSKPVRLSVAIPKASR  179
                V+ + G SKG+GFV F+   E  +A+TE  G + + +KP+ +++A  K  R
Sbjct  4724547  VMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRI-VATKPLYVALAQRKEER  4724398


>PTFA01000232.1 Casuarius casuarius isolate B32419 scaffold_231, whole genome 
shotgun sequence
Length=1555165

 Score = 38.5 bits (88),  Expect = 0.16, Method: Compositional matrix adjust.
 Identities = 28/92 (30%), Positives = 47/92 (51%), Gaps = 4/92 (4%)
 Frame = -2

Query  90      PDN-SPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLD-QTGVSKGYGFVKFTDE  147
               P N S  + +F+ +L  D D+G+L++ F   + +    KV+ D  T   KG+GFV  T+ 
Sbjct  233007  PGNASSGWCIFIYNLGQDADEGILWQMF-GPFGAVTNVKVIRDFNTNKCKGFGFVTMTNY  232831

Query  148     LEQKRALTECQGAVGLGSKPVRLSVAIPKASR  179
                E   A+    G   LG K +++S    K+ +
Sbjct  232830  EEAAMAIASLNGYR-LGDKILQVSFKTNKSHK  232738


>PTFA01000081.1 Casuarius casuarius isolate B32419 scaffold_80, whole genome 
shotgun sequence
Length=3960531

 Score = 37.7 bits (86),  Expect = 0.27, Method: Compositional matrix adjust.
 Identities = 25/85 (29%), Positives = 44/85 (52%), Gaps = 3/85 (4%)
 Frame = +1

Query  96      YSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLD-QTGVSKGYGFVKFTDELEQKRAL  154
               + +FV +L+PD D+ +L++ F   + +    KV+ D  T   KG+GFV  T+  E   A+
Sbjct  154726  WCIFVYNLSPDSDESVLWQLF-GPFGAVNNVKVIRDFNTNKCKGFGFVTMTNYDEAAMAI  154902

Query  155     TECQGAVGLGSKPVRLSVAIPKASR  179
                   G   LG + +++S    K  +
Sbjct  154903  ASLNG-YRLGDRVLQVSFKTNKTHK  154974


>PTFA01000371.1 Casuarius casuarius isolate B32419 scaffold_370, whole genome 
shotgun sequence
Length=717065

 Score = 37.7 bits (86),  Expect = 0.33, Method: Compositional matrix adjust.
 Identities = 25/85 (29%), Positives = 43/85 (51%), Gaps = 3/85 (4%)
 Frame = -2

Query  96      YSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLD-QTGVSKGYGFVKFTDELEQKRAL  154
               + +FV +L PD D+ +L++ F   + +    KV+ D  T   KG+GFV  T+  E   A+
Sbjct  303844  WCIFVYNLAPDADESILWQMF-GPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYDEAAMAI  303668

Query  155     TECQGAVGLGSKPVRLSVAIPKASR  179
                   G   LG + +++S    K  +
Sbjct  303667  ASLNG-YRLGDRVLQVSFKTNKTHK  303596


>PTFA01000093.1 Casuarius casuarius isolate B32419 scaffold_92, whole genome 
shotgun sequence
Length=3726922

 Score = 37.0 bits (84),  Expect = 0.57, Method: Compositional matrix adjust.
 Identities = 25/60 (42%), Positives = 31/60 (52%), Gaps = 4/60 (7%)
 Frame = +3

Query  94       PEYSLFVGDLTPDVDDGMLYEFFVKVYP--SCRGGKVVLDQTGVSKGYGFVKFTDELEQK  151
                P  SL+VGDL PDV + MLYE F    P  S R  + V   T  S GY ++ F    + K
Sbjct  1640787  PLASLYVGDLHPDVTEAMLYEKFSPAGPIMSIRVCRDV--ATRRSLGYAYINFQQPADGK  1640960


 Score = 35.4 bits (80),  Expect = 1.5, Method: Compositional matrix adjust.
 Identities = 19/51 (37%), Positives = 32/51 (63%), Gaps = 1/51 (2%)
 Frame = +2

Query  129      VLDQTGVSKGYGFVKFTDELEQKRALTECQGAVGLGSKPVRLSVAIPKASR  179
                V+ + G SKG+GFV F+   E  +A+TE  G + + +KP+ +++A  K  R
Sbjct  1646660  VMTEGGHSKGFGFVCFSSPEEATKAVTEMNGRI-VSTKPLYVALAQRKEER  1646809


 Score = 35.0 bits (79),  Expect = 2.0, Method: Compositional matrix adjust.
 Identities = 17/50 (34%), Positives = 29/50 (58%), Gaps = 1/50 (2%)
 Frame = +3

Query  3        ASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEF  52
                ASL++GDL P + E  +   F+  G  +MS+++ R+  T    GY ++ F
Sbjct  1640793  ASLYVGDLHPDVTEAMLYEKFSPAGP-IMSIRVCRDVATRRSLGYAYINF  1640939


>PTFA01000051.1 Casuarius casuarius isolate B32419 scaffold_50, whole genome 
shotgun sequence
Length=5510660

 Score = 33.9 bits (76),  Expect = 4.4, Method: Compositional matrix adjust.
 Identities = 22/76 (29%), Positives = 41/76 (54%), Gaps = 8/76 (11%)
 Frame = +1

Query  1        MAASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEK  60
                ++++L++G+L  Y  E  I   F+  G+ V  + +  +++   P G+CFVE+   + A  
Sbjct  4138258  ISSTLYVGNLSFYTTEEQIQELFSKCGD-VKRIVMGLDKIKKTPCGFCFVEYPSWSPAAA  4138434

Query  61       CLHKINGKPLPGATPA  76
                      G+ L GATP+
Sbjct  4138435  ------GR-LLGATPS  4138461


>PTFA01000071.1 Casuarius casuarius isolate B32419 scaffold_70, whole genome 
shotgun sequence
Length=4448226

 Score = 33.5 bits (75),  Expect = 6.8, Method: Compositional matrix adjust.
 Identities = 23/66 (35%), Positives = 33/66 (50%), Gaps = 6/66 (9%)
 Frame = +1

Query  94       PEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQ-TGVSKGYGFVKFTDELEQKR  152
                P  +LFV  L     +  L E F + Y   R  ++V D  TG SKGY F+++ +E    R
Sbjct  4104286  PHLTLFVARLNLQTTEDKLKEVFSR-YGDIRKIRLVRDLVTGFSKGYAFIEYKEE----R  4104450

Query  153      ALTECQ  158
                AL +  
Sbjct  4104451  ALLKAH  4104468



Lambda      K        H        a         alpha
   0.317    0.135    0.406    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 65624718179


  Database: genome.fa
    Posted date:  Oct 19, 2018  9:06 AM
  Number of letters in database: 1,209,001,349
  Number of sequences in database:  3,184



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40
