TBLASTN 2.6.0+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: genome.fa
           3,184 sequences; 1,209,001,349 total letters



Query= SPP00000052_2.0 # Protein # Phosphoseryl-tRNA kinase  (PSTK) # Human

Length=348
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

PTFA01000061.1  Casuarius casuarius isolate B32419 scaffold_60, w...  124     2e-29
PTFA01000020.1  Casuarius casuarius isolate B32419 scaffold_19, w...  33.9    6.0  
PTFA01000214.1  Casuarius casuarius isolate B32419 scaffold_213, ...  33.9    7.3  


>PTFA01000061.1 Casuarius casuarius isolate B32419 scaffold_60, whole genome 
shotgun sequence
Length=5034696

 Score = 124 bits (311),  Expect = 2e-29, Method: Compositional matrix adjust.
 Identities = 61/101 (60%), Positives = 75/101 (74%), Gaps = 2/101 (2%)
 Frame = -1

Query  74       SQWKLLRQELLKYLEYFLMAVINGCQMSVPPNRTEAMWEDFITCLKDQDLIFSAAFEAQS  133
                S+WKL R ELL+YLE+FL A+IN  ++S P +RTE+ WE F+ C K Q L+ S   +A +
Sbjct  4937721  SRWKLYRHELLQYLEHFLQALINRGRLSAPTSRTESTWERFVACCKGQGLLSSQGPDAGA  4937542

Query  134      CYLLTKTAVSRPLFLVLDDNFYYQSMRYEVYQLARK--YSL  172
                C  LT  A SRPL+ +LDDNFYYQSMRYEVYQLARK  YSL
Sbjct  4937541  CQYLTNVATSRPLYFILDDNFYYQSMRYEVYQLARKCNYSL  4937419


 Score = 81.6 bits (200),  Expect = 4e-15, Method: Compositional matrix adjust.
 Identities = 38/64 (59%), Positives = 44/64 (69%), Gaps = 0/64 (0%)
 Frame = -1

Query  171      SLGFCQLFLDCPLETCLQRNGQRPQALPPETIHLMGRKLEKPNPEKNAWEHNSLTIPSPA  230
                SL FCQLFL+CPLE CLQRN  R   LP +TI+LM RK+E P+ E N WE NSL + S  
Sbjct  4937019  SLSFCQLFLECPLEWCLQRNRLRSHPLPDQTIYLMARKIEMPDLENNGWEKNSLILKSFE  4936840

Query  231      CASE  234
                C  E
Sbjct  4936839  CTLE  4936828


 Score = 65.9 bits (159),  Expect = 5e-10, Method: Compositional matrix adjust.
 Identities = 32/51 (63%), Positives = 39/51 (76%), Gaps = 1/51 (2%)
 Frame = -2

Query  14       GPRKRGLCVLCGLPAAGKSTFARALAHRLQQEQGWAIGVVAYDDVM-PDAF  63
                G R+ GLCVLCGLPAAGKST ARAL HRL + Q W   ++ YDD++ P+AF
Sbjct  4938134  GRRRVGLCVLCGLPAAGKSTLARALRHRLPRRQRWDCALLTYDDLIPPEAF  4937982


 Score = 50.1 bits (118),  Expect = 6e-05, Method: Compositional matrix adjust.
 Identities = 20/37 (54%), Positives = 27/37 (73%), Gaps = 0/37 (0%)
 Frame = -3

Query  262      DTDRIICSTNILHKTDQTLRRIVSQTMKEAKGNQEAF  298
                + DR IC+ + +H+ DQT RRI+SQTMK+AKG    F
Sbjct  4935811  EVDRAICAASTVHQADQTFRRIISQTMKDAKGRHSMF  4935701


 Score = 34.3 bits (77),  Expect = 5.2, Method: Compositional matrix adjust.
 Identities = 16/26 (62%), Positives = 19/26 (73%), Gaps = 0/26 (0%)
 Frame = -3

Query  236      SLEVTDLLLTALENPVKYAEDNMEQK  261
                SL++  LL  ALENPVK  E+N EQK
Sbjct  4936372  SLQIVHLLANALENPVKQNEENTEQK  4936295


>PTFA01000020.1 Casuarius casuarius isolate B32419 scaffold_19, whole genome 
shotgun sequence
Length=7916638

 Score = 33.9 bits (76),  Expect = 6.0, Method: Compositional matrix adjust.
 Identities = 24/95 (25%), Positives = 42/95 (44%), Gaps = 4/95 (4%)
 Frame = -2

Query  248      ENPVKYAEDNMEQKDTDRIICSTNILHKTDQTLRRIVSQTMKEAKGNQEAFSEMTFKQR-  306
                +NPV + +++M  KD  R++     LH   +T   +    + +    Q A + +    R 
Sbjct  2539587  QNPV-WTKESM--KDYSRLVLKAFSLHSVRRTACFVFGNKLSQLSETQNASAGLISDDRE  2539417

Query  307      WVRANHAAIWRIILGNEHIKCRSAKVGWLQCCRIE  341
                WV        R+   N  +  + AKV +L CCR +
Sbjct  2539416  WVSDGFLMKDRVSFFNSLVDSKRAKVNYLGCCRFD  2539312


>PTFA01000214.1 Casuarius casuarius isolate B32419 scaffold_213, whole genome 
shotgun sequence
Length=1719536

 Score = 33.9 bits (76),  Expect = 7.3, Method: Compositional matrix adjust.
 Identities = 17/48 (35%), Positives = 23/48 (48%), Gaps = 0/48 (0%)
 Frame = +3

Query  102      VPPNRTEAMWEDFITCLKDQDLIFSAAFEAQSCYLLTKTAVSRPLFLV  149
                V  +R  A W  FI+CL  QD I +A+ +     L  +   S  L LV
Sbjct  1197105  VSFSRARATWSGFISCLNQQDAIIAASLQFSGVALYCQMTAS*SLLLV  1197248



Lambda      K        H        a         alpha
   0.321    0.134    0.417    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 89377036830


  Database: genome.fa
    Posted date:  Oct 19, 2018  9:06 AM
  Number of letters in database: 1,209,001,349
  Number of sequences in database:  3,184



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40
