TBLASTN 2.6.0+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: genome.fa
           3,184 sequences; 1,209,001,349 total letters



Query= SPP00000005_1.0 # Protein # Glutathione peroxidase 2 (GPx2) # Homo
sapiens # Complete

Length=190
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

PTFA01000490.1  Casuarius casuarius isolate B32419 scaffold_489, ...  189     5e-54
PTFA01000489.1  Casuarius casuarius isolate B32419 scaffold_488, ...  168     6e-47
PTFA01000055.1  Casuarius casuarius isolate B32419 scaffold_54, w...  61.2    1e-09
PTFA01000081.1  Casuarius casuarius isolate B32419 scaffold_80, w...  56.6    5e-08
PTFA01000118.1  Casuarius casuarius isolate B32419 scaffold_117, ...  49.7    1e-05
PTFA01000256.1  Casuarius casuarius isolate B32419 scaffold_255, ...  47.0    1e-04
PTFA01000086.1  Casuarius casuarius isolate B32419 scaffold_85, w...  35.4    0.72 
PTFA01000040.1  Casuarius casuarius isolate B32419 scaffold_39, w...  33.1    4.2  
PTFA01000039.1  Casuarius casuarius isolate B32419 scaffold_38, w...  32.0    7.8  


>PTFA01000490.1 Casuarius casuarius isolate B32419 scaffold_489, whole genome 
shotgun sequence
Length=293072

 Score = 189 bits (479),  Expect = 5e-54, Method: Compositional matrix adjust.
 Identities = 83/116 (72%), Positives = 98/116 (84%), Gaps = 0/116 (0%)
 Frame = -2

Query  74      QENCQNEEILNSLKYVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYLKDKLPYPYDDPFSL  133
               QENC NEEILNSLKYVRPGGG++P FTL QKC+VNGQ+ HPVFAYLK  LP P DD   L
Sbjct  150301  QENCTNEEILNSLKYVRPGGGFEPNFTLFQKCQVNGQDTHPVFAYLKAHLPAPADDAVHL  150122

Query  134     MTDPKLIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLKVA  189
               M +P+ ++WSPVRR+D++WNFEKFL+GPEGEPFRRYS   PT  +EPDI+RLLK+A
Sbjct  150121  MAEPRFVVWSPVRRADISWNFEKFLVGPEGEPFRRYSPRMPTAQLEPDIQRLLKLA  149954


 Score = 128 bits (321),  Expect = 8e-33, Method: Compositional matrix adjust.
 Identities = 61/75 (81%), Positives = 66/75 (88%), Gaps = 0/75 (0%)
 Frame = -2

Query  4       IAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLUGTTTRDFTQLNELQCRFPRRLVV  63
               IAKSFYDLSA SL GEKVDFN FRGR VLIENVASL GTT RD+TQLN+LQ R+PRRLVV
Sbjct  150712  IAKSFYDLSATSLQGEKVDFNNFRGRVVLIENVASL*GTTVRDYTQLNQLQARYPRRLVV  150533

Query  64      LGFPCNQFGHQENCQ  78
               LGFPCNQFG+Q + Q
Sbjct  150532  LGFPCNQFGYQVSTQ  150488


>PTFA01000489.1 Casuarius casuarius isolate B32419 scaffold_488, whole genome 
shotgun sequence
Length=288360

 Score = 168 bits (426),  Expect = 6e-47, Method: Compositional matrix adjust.
 Identities = 80/127 (63%), Positives = 102/127 (80%), Gaps = 1/127 (1%)
 Frame = +1

Query  61      LVVLGFPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYLK  120
               LV   FPC  F   EN  NEEIL SL++VRPG GY+P FTL +KC+VNG++ HP+F +LK
Sbjct  152440  LVFCLFPC-LFLS*ENATNEEILRSLEHVRPGNGYKPNFTLFEKCDVNGKDAHPLFTFLK  152616

Query  121     DKLPYPYDDPFSLMTDPKLIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEP  180
               + LP+P+DDP SLMT+P+ IIWSPVRR+D++WNFEKFLIGP+G PF+RYSR F TI I+ 
Sbjct  152617  EALPFPHDDPSSLMTNPQYIIWSPVRRNDISWNFEKFLIGPDGVPFKRYSRHFETIKIQD  152796

Query  181     DIKRLLK  187
               DI+ LL+
Sbjct  152797  DIELLLQ  152817


 Score = 77.8 bits (190),  Expect = 3e-15, Method: Composition-based stats.
 Identities = 37/58 (64%), Positives = 43/58 (74%), Gaps = 1/58 (2%)
 Frame = +1

Query  18      GEKVDFNTFRGRAVLIENVASLUGTTTRDFTQLNELQCRF-PRRLVVLGFPCNQFGHQ  74
                E +  ++  GR +L+ NVASL GTT RDF Q NELQ R+ PR LVVLGFPCNQFGHQ
Sbjct  151948  AEPLALDSLLGRVLLVSNVASL*GTTARDFVQFNELQQRYGPRGLVVLGFPCNQFGHQ  152121


>PTFA01000055.1 Casuarius casuarius isolate B32419 scaffold_54, whole genome 
shotgun sequence
Length=5338051

 Score = 61.2 bits (147),  Expect = 1e-09, Method: Compositional matrix adjust.
 Identities = 34/92 (37%), Positives = 47/92 (51%), Gaps = 6/92 (7%)
 Frame = +1

Query  29       RAVLIENVASLUGTTTRDFTQLNELQCRF-PRRLVVLGFPCNQFGHQENCQNEEILNSLK  87
                +A L+ NVAS +  T +++  L EL   F P    VL FPCNQFG  E   ++EI +  K
Sbjct  2037973  KATLVVNVASYCQHTDKNYIALQELHREFGPSHFTVLAFPCNQFGESEPSSSQEIESFAK  2038152

Query  88       YVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYL  119
                     G Y  TF +  K ++ G    P F +L
Sbjct  2038153  -----GNYGVTFPVFHKIKILGSEAEPAFKFL  2038233


>PTFA01000081.1 Casuarius casuarius isolate B32419 scaffold_80, whole genome 
shotgun sequence
Length=3960531

 Score = 56.6 bits (135),  Expect = 5e-08, Method: Compositional matrix adjust.
 Identities = 34/89 (38%), Positives = 43/89 (48%), Gaps = 6/89 (7%)
 Frame = +3

Query  32       LIENVASLUGTTTRDFTQLNELQCRF-PRRLVVLGFPCNQFGHQENCQNEEILNSLKYVR  90
                L+ NVAS +G T   +  L +LQ    P    VL FPCNQFG QE   N EI +  +   
Sbjct  1168986  LVVNVASECGYTDSHYKALQQLQRDLGPYHFNVLAFPCNQFGQQEPDSNREIESFARKT-  1169162

Query  91       PGGGYQPTFTLVQKCEVNGQNEHPVFAYL  119
                    Y  +F +  K  V+G    P F YL
Sbjct  1169163  ----YGASFPMFSKTAVSGAGAIPAFKYL  1169237


>PTFA01000118.1 Casuarius casuarius isolate B32419 scaffold_117, whole genome 
shotgun sequence
Length=3204144

 Score = 49.7 bits (117),  Expect = 1e-05, Method: Compositional matrix adjust.
 Identities = 21/39 (54%), Positives = 27/39 (69%), Gaps = 1/39 (3%)
 Frame = +1

Query  135     TDPKLIIWSPVRRSDVAWNFEKFLIGPEGEP-FRRYSRT  172
                +PK + W P+R  D+ WNFEKFL+GP+G P  R Y RT
Sbjct  146677  ANPKNLFWEPLRNHDIKWNFEKFLVGPDGVPVMRWYHRT  146793


 Score = 48.5 bits (114),  Expect = 3e-05, Method: Composition-based stats.
 Identities = 44/135 (33%), Positives = 56/135 (41%), Gaps = 47/135 (35%)
 Frame = +3

Query  4       IAKSFYDLSAISLDGEK-VDFNTFRGRAVLIENVASLUGTTTRDF---------------  47
               +  + YD  A+++DGE+ V F  + G+ VL  NVA+  G T +                 
Sbjct  145719  VQGTIYDYGALTIDGEEYVPFKKYAGKMVLFVNVATY*GLTLQYLGKEAAAAAGPPRQRL  145898

Query  48      -----------------------------TQLNELQCRF-PRRLVVLGFPCNQFGHQENC  77
                                             +LN LQ    P  LVVLGFP NQFG QE  
Sbjct  145899  SGQGGGHIGGGPAWVR*LLR*PRPWPCLPAELNALQNELGPYGLVVLGFPSNQFGKQEPG  146078

Query  78      QNEEILNSLKYV-RP  91
               QN EIL +LK V RP
Sbjct  146079  QNSEILPALK*VPRP  146123


 Score = 47.4 bits (111),  Expect = 7e-05, Method: Composition-based stats.
 Identities = 20/38 (53%), Positives = 27/38 (71%), Gaps = 0/38 (0%)
 Frame = +3

Query  83      LNSLKYVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYLK  120
               L+  +YVRPGGG+ P F L QK +VNG  E  V+++LK
Sbjct  146433  LSPCRYVRPGGGFVPNFQLFQKGDVNGAREQKVYSFLK  146546


>PTFA01000256.1 Casuarius casuarius isolate B32419 scaffold_255, whole genome 
shotgun sequence
Length=1427201

 Score = 47.0 bits (110),  Expect = 1e-04, Method: Compositional matrix adjust.
 Identities = 25/49 (51%), Positives = 31/49 (63%), Gaps = 1/49 (2%)
 Frame = +3

Query  27      RGRAVLIENVASLUGTTTRDFTQLNELQCRFPRR-LVVLGFPCNQFGHQ  74
               RG   +I NVAS  G T  ++TQL +L  R+  R L +L FPCNQFG Q
Sbjct  735783  RGFVCIITNVASK*GKTDVNYTQLVDLYARYAERGLRILAFPCNQFGKQ  735929


>PTFA01000086.1 Casuarius casuarius isolate B32419 scaffold_85, whole genome 
shotgun sequence
Length=3830250

 Score = 35.4 bits (80),  Expect = 0.72, Method: Compositional matrix adjust.
 Identities = 30/108 (28%), Positives = 48/108 (44%), Gaps = 19/108 (18%)
 Frame = +2

Query  61       LVVLGFPC----NQFGHQENCQNEEILNSLKYVRPGG------GYQPTFTLVQKCEVNGQ  110
                L+ LG  C     QF  Q++ +N E +   K+V+ G       G   TF LVQ+ ++   
Sbjct  2050523  LLFLGIHCLLLNEQFLLQQHSENTEKVI*SKFVKLGSCEFSLRGKSSTFILVQEGKLLQ*  2050702

Query  111      NEHPVFAYLKDKLPYPYDDPFSLMTDPKLIIWSPVRRSDVAWNFEKFL  158
                N+         K  YP    F      + +++S V ++D  W F+K L
Sbjct  2050703  NK---------KHKYPLSLEFHYELRSQFMVYSFV*KADETWKFQKTL  2050819


>PTFA01000040.1 Casuarius casuarius isolate B32419 scaffold_39, whole genome 
shotgun sequence
Length=6115981

 Score = 33.1 bits (74),  Expect = 4.2, Method: Compositional matrix adjust.
 Identities = 15/46 (33%), Positives = 23/46 (50%), Gaps = 0/46 (0%)
 Frame = +3

Query  54      QCRFPRRLVVLGFPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTF  99
               QC F R++ + G  C +      C  + I  SL+ VRP G + P +
Sbjct  272850  QCDFTRKMYLTGSQCKKIFSC*LCSEQHIALSLRQVRPHGAHTPVW  272987


>PTFA01000039.1 Casuarius casuarius isolate B32419 scaffold_38, whole genome 
shotgun sequence
Length=6135250

 Score = 32.0 bits (71),  Expect = 7.8, Method: Compositional matrix adjust.
 Identities = 21/59 (36%), Positives = 27/59 (46%), Gaps = 8/59 (14%)
 Frame = -3

Query  31       VLIENVASLUGT--------TTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQENCQNEE  81
                +L+E+  S+ GT          R F  LN   C FP  L+ L F    FGH+  C  EE
Sbjct  3861863  LLLESKPSMSGT*KFLLENSKCRIFILLNLSVCFFPE*LLTLNFVFFLFGHKFTCDYEE  3861687



Lambda      K        H        a         alpha
   0.324    0.142    0.443    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 28988981064


  Database: genome.fa
    Posted date:  Oct 19, 2018  9:06 AM
  Number of letters in database: 1,209,001,349
  Number of sequences in database:  3,184



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40
