TBLASTN 2.6.0+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: genome.fa
           3,184 sequences; 1,209,001,349 total letters



Query= SPP00000004_1.0 # Protein # Glutathione peroxidase 1 (GPx1) # Homo
sapiens # Complete

Length=203
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

PTFA01000489.1  Casuarius casuarius isolate B32419 scaffold_488, ...  178     3e-50
PTFA01000490.1  Casuarius casuarius isolate B32419 scaffold_489, ...  170     3e-47
PTFA01000081.1  Casuarius casuarius isolate B32419 scaffold_80, w...  74.7    5e-14
PTFA01000055.1  Casuarius casuarius isolate B32419 scaffold_54, w...  70.9    9e-13
PTFA01000118.1  Casuarius casuarius isolate B32419 scaffold_117, ...  55.8    1e-07
PTFA01000256.1  Casuarius casuarius isolate B32419 scaffold_255, ...  53.9    5e-07
PTFA01000202.1  Casuarius casuarius isolate B32419 scaffold_201, ...  35.8    0.030
PTFA01000542.1  Casuarius casuarius isolate B32419 scaffold_541, ...  34.7    1.6  


>PTFA01000489.1 Casuarius casuarius isolate B32419 scaffold_488, whole genome 
shotgun sequence
Length=288360

 Score = 178 bits (452),  Expect = 3e-50, Method: Compositional matrix adjust.
 Identities = 85/131 (65%), Positives = 102/131 (78%), Gaps = 1/131 (1%)
 Frame = +1

Query  71      LVVLGFPCNQFGHQENAKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLR  130
               LV   FPC  F   ENA NEEIL SL++VRPG G++PNF LFEKC+VNG  AHPLF FL+
Sbjct  152440  LVFCLFPC-LFLS*ENATNEEILRSLEHVRPGNGYKPNFTLFEKCDVNGKDAHPLFTFLK  152616

Query  131     EALPAPSDDATALMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEP  190
               EALP P DD ++LMT+P+ I WSPV RND++WNFEKFL+GPDGVP +RYSR F+TI I+ 
Sbjct  152617  EALPFPHDDPSSLMTNPQYIIWSPVRRNDISWNFEKFLIGPDGVPFKRYSRHFETIKIQD  152796

Query  191     DIEALLSQGPS  201
               DIE LL + P 
Sbjct  152797  DIELLLQKVPK  152829


 Score = 99.4 bits (246),  Expect = 1e-22, Method: Compositional matrix adjust.
 Identities = 47/69 (68%), Positives = 54/69 (78%), Gaps = 0/69 (0%)
 Frame = +1

Query  16      VYAFSARPLAGGEPVSLGSLRGKVLLIENVASLUGTTVRDYTQMNELQRRLGPRGLVVLG  75
               +    ARPL   EP++L SL G+VLL+ NVASL GTT RD+ Q NELQ+R GPRGLVVLG
Sbjct  151915  LAGLQARPLGAAEPLALDSLLGRVLLVSNVASL*GTTARDFVQFNELQQRYGPRGLVVLG  152094

Query  76      FPCNQFGHQ  84
               FPCNQFGHQ
Sbjct  152095  FPCNQFGHQ  152121


>PTFA01000490.1 Casuarius casuarius isolate B32419 scaffold_489, whole genome 
shotgun sequence
Length=293072

 Score = 170 bits (430),  Expect = 3e-47, Method: Compositional matrix adjust.
 Identities = 76/114 (67%), Positives = 90/114 (79%), Gaps = 0/114 (0%)
 Frame = -2

Query  84      QENAKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDATAL  143
               QEN  NEEILNSLKYVRPGGGFEPNF LF+KC+VNG   HP+FA+L+  LPAP+DDA  L
Sbjct  150301  QENCTNEEILNSLKYVRPGGGFEPNFTLFQKCQVNGQDTHPVFAYLKAHLPAPADDAVHL  150122

Query  144     MTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEPDIEALLS  197
               M +P+ + WSPV R D++WNFEKFLVGP+G P RRYS R  T  +EPDI+ LL 
Sbjct  150121  MAEPRFVVWSPVRRADISWNFEKFLVGPEGEPFRRYSPRMPTAQLEPDIQRLLK  149960


 Score = 87.4 bits (215),  Expect = 2e-18, Method: Compositional matrix adjust.
 Identities = 47/71 (66%), Positives = 54/71 (76%), Gaps = 2/71 (3%)
 Frame = -2

Query  14      QSVYAFSARPLAGGEPVSLGSLRGKVLLIENVASLUGTTVRDYTQMNELQRRLGPRGLVV  73
               +S Y  SA  L G E V   + RG+V+LIENVASL GTTVRDYTQ+N+LQ R  PR LVV
Sbjct  150706  KSFYDLSATSLQG-EKVDFNNFRGRVVLIENVASL*GTTVRDYTQLNQLQARY-PRRLVV  150533

Query  74      LGFPCNQFGHQ  84
               LGFPCNQFG+Q
Sbjct  150532  LGFPCNQFGYQ  150500


>PTFA01000081.1 Casuarius casuarius isolate B32419 scaffold_80, whole genome 
shotgun sequence
Length=3960531

 Score = 74.7 bits (182),  Expect = 5e-14, Method: Compositional matrix adjust.
 Identities = 42/91 (46%), Positives = 52/91 (57%), Gaps = 5/91 (5%)
 Frame = +3

Query  39       VLLIENVASLUGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNSLKY  98
                V L+ NVAS +G T   Y  + +LQR LGP    VL FPCNQFG QE   N EI +   +
Sbjct  1168980  VSLVVNVASECGYTDSHYKALQQLQRDLGPYHFNVLAFPCNQFGQQEPDSNREIES---F  1169150

Query  99       VRPGGGFEPNFMLFEKCEVNGAGAHPLFAFL  129
                 R   G   +F +F K  V+GAGA P F +L
Sbjct  1169151  ARKTYG--ASFPMFSKTAVSGAGAIPAFKYL  1169237


>PTFA01000055.1 Casuarius casuarius isolate B32419 scaffold_54, whole genome 
shotgun sequence
Length=5338051

 Score = 70.9 bits (172),  Expect = 9e-13, Method: Compositional matrix adjust.
 Identities = 36/92 (39%), Positives = 50/92 (54%), Gaps = 5/92 (5%)
 Frame = +1

Query  38       KVLLIENVASLUGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNSLK  97
                K  L+ NVAS +  T ++Y  + EL R  GP    VL FPCNQFG  E + ++EI +  K
Sbjct  2037973  KATLVVNVASYCQHTDKNYIALQELHREFGPSHFTVLAFPCNQFGESEPSSSQEIESFAK  2038152

Query  98       YVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFL  129
                     G +   F +F K ++ G+ A P F FL
Sbjct  2038153  -----GNYGVTFPVFHKIKILGSEAEPAFKFL  2038233


>PTFA01000118.1 Casuarius casuarius isolate B32419 scaffold_117, whole genome 
shotgun sequence
Length=3204144

 Score = 55.8 bits (133),  Expect = 1e-07, Method: Compositional matrix adjust.
 Identities = 23/49 (47%), Positives = 32/49 (65%), Gaps = 0/49 (0%)
 Frame = +1

Query  144     MTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEPDI  192
                 +PK + W P+  +D+ WNFEKFLVGPDGVP+ R+  R     ++ DI
Sbjct  146674  FANPKNLFWEPLRNHDIKWNFEKFLVGPDGVPVMRWYHRTNIAVVKNDI  146820


 Score = 54.7 bits (130),  Expect = 3e-07, Method: Compositional matrix adjust.
 Identities = 26/41 (63%), Positives = 30/41 (73%), Gaps = 0/41 (0%)
 Frame = +3

Query  57      TQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNSLK  97
                ++N LQ  LGP GLVVLGFP NQFG QE  +N EIL +LK
Sbjct  145986  AELNALQNELGPYGLVVLGFPSNQFGKQEPGQNSEILPALK  146108


 Score = 48.5 bits (114),  Expect = 3e-05, Method: Compositional matrix adjust.
 Identities = 21/38 (55%), Positives = 29/38 (76%), Gaps = 0/38 (0%)
 Frame = +3

Query  93      LNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLR  130
               L+  +YVRPGGGF PNF LF+K +VNGA    +++FL+
Sbjct  146433  LSPCRYVRPGGGFVPNFQLFQKGDVNGAREQKVYSFLK  146546


>PTFA01000256.1 Casuarius casuarius isolate B32419 scaffold_255, whole genome 
shotgun sequence
Length=1427201

 Score = 53.9 bits (128),  Expect = 5e-07, Method: Compositional matrix adjust.
 Identities = 27/49 (55%), Positives = 32/49 (65%), Gaps = 0/49 (0%)
 Frame = +3

Query  36      RGKVLLIENVASLUGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQ  84
               RG V +I NVAS  G T  +YTQ+ +L  R   RGL +L FPCNQFG Q
Sbjct  735783  RGFVCIITNVASK*GKTDVNYTQLVDLYARYAERGLRILAFPCNQFGKQ  735929


>PTFA01000202.1 Casuarius casuarius isolate B32419 scaffold_201, whole genome 
shotgun sequence
Length=1887227

 Score = 35.8 bits (81),  Expect(2) = 0.030, Method: Compositional matrix adjust.
 Identities = 14/31 (45%), Positives = 21/31 (68%), Gaps = 0/31 (0%)
 Frame = +2

Query  84      QENAKNEEILNSLKYVRPGGGFEPNFMLFEK  114
               Q N +N    +S  Y+RPGGGF P+F+ F++
Sbjct  436550  QRNVRNCGYSSSYMYIRPGGGFFPHFVCFQR  436642


 Score = 22.7 bits (47),  Expect(2) = 0.030, Method: Compositional matrix adjust.
 Identities = 8/26 (31%), Positives = 16/26 (62%), Gaps = 0/26 (0%)
 Frame = +1

Query  133     LPAPSDDATALMTDPKLITWSPVCRN  158
               LPAP   A +++   +L+ W+ + R+
Sbjct  436762  LPAPYGSAKSILQREELVPWTNLGRS  436839


>PTFA01000542.1 Casuarius casuarius isolate B32419 scaffold_541, whole genome 
shotgun sequence
Length=180124

 Score = 34.7 bits (78),  Expect = 1.6, Method: Compositional matrix adjust.
 Identities = 27/77 (35%), Positives = 35/77 (45%), Gaps = 13/77 (17%)
 Frame = +2

Query  55     DYTQMNELQRRLGPRGLVVL--GF----PC---NQFGHQENAKNEEIL----NSLKYVRP  101
              DY Q  +L + LGP   V L  GF    PC      GH    K+  ++    N LK+ RP
Sbjct  49493  DYVQE*QLSKLLGPHMSVDLPSGFSSVIPCFTQQGEGHVHTLKDAGLVSVAKNQLKFDRP  49672

Query  102    GGGFEPNFMLFEKCEVN  118
              GG + P +   E C V 
Sbjct  49673  GGEWAPEWAQMENCPVQ  49723



Lambda      K        H        a         alpha
   0.321    0.139    0.433    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 33820210452


  Database: genome.fa
    Posted date:  Oct 19, 2018  9:06 AM
  Number of letters in database: 1,209,001,349
  Number of sequences in database:  3,184



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40
