TBLASTN 2.6.0+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: genome.fa
           3,184 sequences; 1,209,001,349 total letters



Query= EFSec

Length=596
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

PTFA01000138.1  Casuarius casuarius isolate B32419 scaffold_137, ...  348     2e-103
PTFA01000025.1  Casuarius casuarius isolate B32419 scaffold_24, w...  43.9    0.012 
PTFA01000092.1  Casuarius casuarius isolate B32419 scaffold_91, w...  42.0    0.052 
PTFA01000714.1  Casuarius casuarius isolate B32419 scaffold_713, ...  39.3    0.32  
PTFA01000118.1  Casuarius casuarius isolate B32419 scaffold_117, ...  36.2    3.0   
PTFA01000002.1  Casuarius casuarius isolate B32419 scaffold_1, wh...  34.7    7.9   
PTFA01000269.1  Casuarius casuarius isolate B32419 scaffold_268, ...  34.7    8.1   


>PTFA01000138.1 Casuarius casuarius isolate B32419 scaffold_137, whole genome 
shotgun sequence
Length=2905401

 Score = 348 bits (894),  Expect = 2e-103, Method: Compositional matrix adjust.
 Identities = 164/235 (70%), Positives = 194/235 (83%), Gaps = 9/235 (4%)
 Frame = +1

Query  263      VVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCAPESLHTVHAALISVEKI  322
                V KKVKSMQMFH P+  AMQGDR+GICVTQFDPKLLERGL+C PESLHT+HAA+IS++KI
Sbjct  2617888  VTKKVKSMQMFHTPVAYAMQGDRVGICVTQFDPKLLERGLICTPESLHTIHAAIISLKKI  2618067

Query  323      PYFRGPLQTKAKFHITVGHETVMGRLMFFSPAPDNFDQEPILDSFNFSQEYLFQEQYLSK  382
                 YFRG LQTKAKFHITVGHETVMGR+MFFSPAP +F++E   + FNF ++YL+QE+YLSK
Sbjct  2618068  QYFRGALQTKAKFHITVGHETVMGRVMFFSPAPADFNEEIQENVFNFEKDYLYQEEYLSK  2618247

Query  383      DLTPAVTDNDEADKKAGQATEGHCPRQQWALVEFEKPVTCPRLCLVIGSRLDADIHTNTC  442
                D     +   E +KK  Q +E   PRQQWAL+EFEKPVTCP+LCLVIGS+LD DIH NTC
Sbjct  2618248  D-----SKTSEENKKNDQ-SEVQLPRQQWALLEFEKPVTCPKLCLVIGSKLDTDIHANTC  2618409

Query  443      RLAFHGILLHGLEDRNYADSFLPRLKVYKLKHKHGLVERAMDDYSVI---GRSLF  494
                RLAFHG+LL G+ED+NYA++FLP+LKVYKLKHK G VER    +S I   G+SLF
Sbjct  2618410  RLAFHGVLLQGMEDKNYAETFLPKLKVYKLKHKEGQVERVSCLFSAIHGKGKSLF  2618574


 Score = 134 bits (337),  Expect = 5e-31, Method: Compositional matrix adjust.
 Identities = 69/82 (84%), Positives = 75/82 (91%), Gaps = 2/82 (2%)
 Frame = +1

Query  96       HASLIRTIIG--GAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNKIDLLPE  153
                +A+ I T+    GAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQK+VVVLNKIDLLPE
Sbjct  2579806  YAATILTVNSPPGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKMVVVLNKIDLLPE  2579985

Query  154      GKRQAAIDKMTKKMQKTLENTK  175
                 KRQ+AI+KMTKKMQKTLENTK
Sbjct  2579986  SKRQSAIEKMTKKMQKTLENTK  2580051


 Score = 98.6 bits (244),  Expect = 2e-19, Method: Compositional matrix adjust.
 Identities = 46/59 (78%), Positives = 53/59 (90%), Gaps = 0/59 (0%)
 Frame = +1

Query  205      LIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKV  263
                  ++L SQ  +P+RDPSG FLM+VDHCFSIKGQGTVMTGTILSGS+SLGD+VEIPALKV
Sbjct  2586868  FFQVLKSQAYLPSRDPSGHFLMAVDHCFSIKGQGTVMTGTILSGSVSLGDNVEIPALKV  2587044


 Score = 93.2 bits (230),  Expect = 7e-18, Method: Compositional matrix adjust.
 Identities = 44/54 (81%), Positives = 48/54 (89%), Gaps = 0/54 (0%)
 Frame = +1

Query  481      RAMDDYSVIGRSLFKKETNIQLFVGLKVHLSTGELGIIDSAFGQSGKFKIHIPG  534
                + MDDYSVIGRSLFKKETNIQ+FVGLKV LSTGE G+I+  FGQSGKFKI IPG
Sbjct  2633023  QVMDDYSVIGRSLFKKETNIQIFVGLKVKLSTGEDGVIEGGFGQSGKFKIRIPG  2633184


 Score = 77.0 bits (188),  Expect = 7e-13, Method: Compositional matrix adjust.
 Identities = 60/90 (67%), Positives = 62/90 (69%), Gaps = 15/90 (17%)
 Frame = +2

Query  13       LGHIDSGKtalaralsttastaaFDKQPQSRERGITLDLGFSCFSVPLPARLRSSlpefq  72
                LGHIDSGKTALARALSTT STAAFD+ PQSR RGITLDLGFSC    LP +L        
Sbjct  2546231  LGHIDSGKTALARALSTTGSTAAFDRAPQSRARGITLDLGFSCLRTALPPQL--------  2546386

Query  73       aapeaepepgepllQVTLVDCPGHASLIRT  102
                        PG   LQ TLVDCPGHASLIRT
Sbjct  2546387  -------GPGPGELQFTLVDCPGHASLIRT  2546455


 Score = 52.0 bits (123),  Expect = 4e-05, Method: Compositional matrix adjust.
 Identities = 28/66 (42%), Positives = 48/66 (73%), Gaps = 1/66 (2%)
 Frame = +1

Query  530      IHIPGGLSPESKKILTPALKKRARAGRGEATRQEESAERSEPSQHVVLSLTFKRYVFDTH  589
                +    GL P++K +LT A KK+++A +G+A +++E + +S+ +Q V ++L FKRYVFD  
Sbjct  2673598  LFFSDGLRPDTKMLLTVASKKKSKASKGDAAKEDEGS-KSDSAQPVAITLLFKRYVFDMQ  2673774

Query  590      KRMVQS  595
                K+M+QS
Sbjct  2673775  KKMIQS  2673792


>PTFA01000025.1 Casuarius casuarius isolate B32419 scaffold_24, whole genome 
shotgun sequence
Length=7387781

 Score = 43.9 bits (102),  Expect = 0.012, Method: Compositional matrix adjust.
 Identities = 25/74 (34%), Positives = 37/74 (50%), Gaps = 0/74 (0%)
 Frame = -1

Query  232      FSIKGQGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQMFHMPITSAMQGDRLGICVT  291
                FS  G GTV  G + +G +  G  V    + V  +VKS++M H  ++ A+ GD +G  V 
Sbjct  5474102  FSFTGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVK  5473923

Query  292      QFDPKLLERGLVCA  305
                    K + RG V  
Sbjct  5473922  NVSVKDVRRGNVAG  5473881


>PTFA01000092.1 Casuarius casuarius isolate B32419 scaffold_91, whole genome 
shotgun sequence
Length=3751510

 Score = 42.0 bits (97),  Expect = 0.052, Method: Compositional matrix adjust.
 Identities = 23/70 (33%), Positives = 36/70 (51%), Gaps = 0/70 (0%)
 Frame = +3

Query  236      GQGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDP  295
                G GTV  G + +G +  G  V    + +  +VKS++M H  ++ A+ GD +G  V     
Sbjct  1254654  GIGTVPVGRVETGILRPGMVVTFAPVNITTEVKSVEMHHEALSEALPGDNVGFNVKNVSV  1254833

Query  296      KLLERGLVCA  305
                K + RG VC 
Sbjct  1254834  KDIRRGNVCG  1254863


>PTFA01000714.1 Casuarius casuarius isolate B32419 scaffold_713, whole genome 
shotgun sequence
Length=70043

 Score = 39.3 bits (90),  Expect = 0.32, Method: Compositional matrix adjust.
 Identities = 36/154 (23%), Positives = 58/154 (38%), Gaps = 56/154 (36%)
 Frame = -1

Query  204    ELIELLTSQISIPTRDPSGPFLMSVDHCFSI--------------------------KGQ  237
              +L+E + + I +P RD   PFL+ V+   SI                           G+
Sbjct  38552  KLLEAIDTHIPLPVRDLDKPFLLPVESVHSIPGRSCPPAAPRRLPPPPGPEPPLSVPAGR  38373

Query  238    GTVMTGTILSGSISLGDSVEIPALKVVKKVKS----------------------------  269
              GTV+TGT+  G+I  GD  E   L   + +++                            
Sbjct  38372  GTVVTGTLERGTIRKGDECEF--LGYNRNLRTVVTGGRPPPPPALAPRRPRGADPLLPAG  38199

Query  270    MQMFHMPITSAMQGDRLGICVTQFDPKLLERGLV  303
              ++MFH  +  A  GD LG  +     + + RG+V
Sbjct  38198  IEMFHKSLERAEAGDNLGALLRGLKREDVRRGMV  38097


>PTFA01000118.1 Casuarius casuarius isolate B32419 scaffold_117, whole genome 
shotgun sequence
Length=3204144

 Score = 36.2 bits (82),  Expect = 3.0, Method: Compositional matrix adjust.
 Identities = 30/87 (34%), Positives = 41/87 (47%), Gaps = 8/87 (9%)
 Frame = +2

Query  288      ICVTQ--FDPKLLERGLVC---APESLHTVHAALISVEKIPYFR-GPLQTKAKFHITVGH  341
                IC  Q  F+P  LER  VC   +P+ L +  ++ IS+E +  FR  PL  +  +      
Sbjct  1875185  ICSPQAPFNPVCLERVCVCLPISPQGLQSAASSHISMEYLDIFRLLPLGFQLSWKNKTWS  1875364

Query  342      ETVMGRLMFFSP--APDNFDQEPILDS  366
                   +GRLMF  P      F  EP L S
Sbjct  1875365  WPQVGRLMFSGPYLCAGPFSPEPALAS  1875445


>PTFA01000002.1 Casuarius casuarius isolate B32419 scaffold_1, whole genome shotgun 
sequence
Length=14296948

 Score = 34.7 bits (78),  Expect = 7.9, Method: Compositional matrix adjust.
 Identities = 14/28 (50%), Positives = 19/28 (68%), Gaps = 0/28 (0%)
 Frame = -3

Query  89       TLVDCPGHASLIRTIIGGAQIIDLMMLV  116
                T++D PGH S +  +IGGA   DL +LV
Sbjct  6022580  TILDAPGHKSFVPNMIGGASQADLAVLV  6022497


>PTFA01000269.1 Casuarius casuarius isolate B32419 scaffold_268, whole genome 
shotgun sequence
Length=1317000

 Score = 34.7 bits (78),  Expect = 8.1, Method: Compositional matrix adjust.
 Identities = 14/31 (45%), Positives = 22/31 (71%), Gaps = 0/31 (0%)
 Frame = +2

Query  87      QVTLVDCPGHASLIRTIIGGAQIIDLMMLVI  117
                V+ VDCPGH  L+ T++ GA ++D  +L+I
Sbjct  158585  HVSFVDCPGHDILMATMLNGAAVMDAALLLI  158677



Lambda      K        H        a         alpha
   0.321    0.137    0.401    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 187201255425


  Database: genome.fa
    Posted date:  Oct 19, 2018  9:06 AM
  Number of letters in database: 1,209,001,349
  Number of sequences in database:  3,184



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40
