TBLASTN 2.6.0+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: genome.fa
           3,184 sequences; 1,209,001,349 total letters



Query= SPP00000002_1.0 # Protein # Iodothyronine deiodinase 2 (DI2) # Homo
sapiens # Complete

Length=265
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

PTFA01000143.1  Casuarius casuarius isolate B32419 scaffold_142, ...  304     4e-93
PTFA01000029.1  Casuarius casuarius isolate B32419 scaffold_28, w...  169     5e-46
PTFA01000081.1  Casuarius casuarius isolate B32419 scaffold_80, w...  58.5    4e-08
PTFA01000207.1  Casuarius casuarius isolate B32419 scaffold_206, ...  34.3    3.0  
PTFA01000133.1  Casuarius casuarius isolate B32419 scaffold_132, ...  32.7    8.4  


>PTFA01000143.1 Casuarius casuarius isolate B32419 scaffold_142, whole genome 
shotgun sequence
Length=2780045

 Score = 304 bits (779),  Expect = 4e-93, Method: Compositional matrix adjust.
 Identities = 152/193 (79%), Positives = 166/193 (86%), Gaps = 3/193 (2%)
 Frame = +2

Query  74      QVKLGEDAPNSSVVHVSSTEGGDNSGNGTQEKIAE-GATCHLLDFASPERPLVVNFGSAT  132
               QVKLG +APNSSV+HV++  G D S N  +    + G+ CHLLDFA+ ERPLVVNFGSAT
Sbjct  674180  QVKLGGEAPNSSVIHVAN--GSDRSTNSWKSVGGKCGSKCHLLDFANSERPLVVNFGSAT  674353

Query  133     UPPFTSQLPAFRKLVEEFSSVADFLLVYIDEAHPSDGWAIPGDSSLSFEVKKHQNQEDRC  192
                PPFT+QL AF KLVEEFS VADFLLVYIDEAHPSDGWA PG S  SFEVKKH+NQEDRC
Sbjct  674354  *PPFTNQLSAFSKLVEEFSGVADFLLVYIDEAHPSDGWAAPGISPSSFEVKKHRNQEDRC  674533

Query  193     AAAQQLLERFSLPPQCRVVADRMDNNANIAYGVAFERVCIVQRQKIAYLGGKGPFSYNLQ  252
               AAA QLLERFSLPPQC+VVAD MDNNAN+AYGV+FERVCIVQRQKIAYLGGKGPF YNLQ
Sbjct  674534  AAAHQLLERFSLPPQCQVVADCMDNNANVAYGVSFERVCIVQRQKIAYLGGKGPFFYNLQ  674713

Query  253     EVRHWLEKNFSKR  265
               EVR WLE+NFSKR
Sbjct  674714  EVRLWLEQNFSKR  674752


 Score = 144 bits (364),  Expect = 2e-37, Method: Compositional matrix adjust.
 Identities = 69/75 (92%), Positives = 72/75 (96%), Gaps = 0/75 (0%)
 Frame = +3

Query  1       MGILSVDLLITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEGL  60
               MG+LSVDLLITLQILPVFFSNCLFLALYDSVILLKH+VL LSRSK+ RGEWRRMLTSEGL
Sbjct  662022  MGLLSVDLLITLQILPVFFSNCLFLALYDSVILLKHMVLFLSRSKAARGEWRRMLTSEGL  662201

Query  61      RCVWKSFLLDAYKQV  75
               RCVW SFLLDAYKQV
Sbjct  662202  RCVWNSFLLDAYKQV  662246


>PTFA01000029.1 Casuarius casuarius isolate B32419 scaffold_28, whole genome 
shotgun sequence
Length=6902520

 Score = 169 bits (428),  Expect = 5e-46, Method: Compositional matrix adjust.
 Identities = 87/208 (42%), Positives = 123/208 (59%), Gaps = 23/208 (11%)
 Frame = -2

Query  53       RMLTSEGLRCVWKSFLLDAYKQVKLGEDAPNSSVVHVSSTEGGDNSGNGTQEKIAEGATC  112
                RM T E L+ VW    LD +K   +G  APN  V+ +             Q+++      
Sbjct  2958065  RMFTLESLKAVWHGQKLDFFKSAHVGSLAPNPEVIQLDG-----------QKRL------  2957937

Query  113      HLLDFASPERPLVVNFGSATUPPFTSQLPAFRKLVEEFSSVADFLLVYIDEAHPSDGWAI  172
                 +LDFA  +RPL++NFGS T PPF ++L +FR+L   F  +ADFLLVYI+EAHPSDGW  
Sbjct  2957936  RILDFARGKRPLILNFGSCT*PPFMARLRSFRRLAAHFVDIADFLLVYIEEAHPSDGWV-  2957760

Query  173      PGDSSLSFEVKKHQNQEDRCAAAQQLLERFSLPPQCRVVADRMDNNANIAYGVAFERVCI  232
                   S  ++++ KHQ  +DR  AAQ + E     P C +  D MDN ++ AYG  FER+ I
Sbjct  2957759  --SSDAAYKIPKHQCLQDRLRAAQLMREG---APDCPLAVDTMDNASSAAYGAYFERLYI  2957595

Query  233      VQRQKIAYLGGKGPFSYNLQEVRHWLEK  260
                +Q +K+ Y GG+GP  Y + E+R WL++
Sbjct  2957594  IQEEKVMYQGGRGPEGYKISELRSWLDQ  2957511


>PTFA01000081.1 Casuarius casuarius isolate B32419 scaffold_80, whole genome 
shotgun sequence
Length=3960531

 Score = 58.5 bits (140),  Expect = 4e-08, Method: Compositional matrix adjust.
 Identities = 28/56 (50%), Positives = 39/56 (70%), Gaps = 1/56 (2%)
 Frame = +1

Query  110      ATCHLLDFASPERPLVVNFGSATUPPFTSQLPAFRKLVEEFSSVADFLLVYIDEAH  165
                +  H+L F    RPL++NFGS T P F  +   F KL+++FSS ADFL++YI+EAH
Sbjct  2024446  SVSHVL-FPPDNRPLILNFGSCT*PSFMLKFDEFNKLIKDFSSXADFLIIYIEEAH  2024610


 Score = 47.4 bits (111),  Expect = 2e-04, Method: Compositional matrix adjust.
 Identities = 28/73 (38%), Positives = 40/73 (55%), Gaps = 7/73 (10%)
 Frame = +3

Query  168      DGWAIPGDSSLSFEVKKHQNQEDRCAAAQQLLERFSLPPQCRVVADRMDNNANIAYGVAF  227
                DGWA   +      +K H++ EDR  AAQ LLE+    P C VV D M+N ++  Y    
Sbjct  2025846  DGWAFKNNVV----IKNHRSLEDRRTAAQFLLEK---NPLCPVVLDTMENLSSSKYAALP  2026004

Query  228      ERVCIVQRQKIAY  240
                ER+ ++Q  K+ Y
Sbjct  2026005  ERLYLLQGGKVIY  2026043


>PTFA01000207.1 Casuarius casuarius isolate B32419 scaffold_206, whole genome 
shotgun sequence
Length=1799873

 Score = 34.3 bits (77),  Expect = 3.0, Method: Compositional matrix adjust.
 Identities = 17/55 (31%), Positives = 23/55 (42%), Gaps = 0/55 (0%)
 Frame = -3

Query  112      CHLLDFASPERPLVVNFGSATUPPFTSQLPAFRKLVEEFSSVADFLLVYIDEAHP  166
                CHL   +SP +P     G A +PP    L   R    + SS  + L   +  A P
Sbjct  1022739  CHLAAGSSPRQPA*PGHGRAACPPSPGCLHPQRPPASQLSSFGEILKPRVPRAAP  1022575


>PTFA01000133.1 Casuarius casuarius isolate B32419 scaffold_132, whole genome 
shotgun sequence
Length=3014014

 Score = 32.7 bits (73),  Expect = 8.4, Method: Compositional matrix adjust.
 Identities = 21/74 (28%), Positives = 30/74 (41%), Gaps = 3/74 (4%)
 Frame = +2

Query  150      FSSVADFLLVYIDEAHPSDGWAIPGDSSLSFEVKKHQNQEDRCAAAQQLLERFSLPPQCR  209
                FS V    + Y D + P  G+  PG   LSF V  H N        Q+L     + P  R
Sbjct  1703342  FSCVPGSYVCYADVSVPLPGFKCPGSIRLSFLVTIHVNPAH---LNQELSSALGIHPSTR  1703512

Query  210      VVADRMDNNANIAY  223
                   ++  +N  + Y
Sbjct  1703513  TQEEKKKHNVTMIY  1703554



Lambda      K        H        a         alpha
   0.321    0.136    0.410    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 57170452014


  Database: genome.fa
    Posted date:  Oct 19, 2018  9:06 AM
  Number of letters in database: 1,209,001,349
  Number of sequences in database:  3,184



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40
