_________________________________.______________________._____________________________________________________________________________ _|*|_|*|_|*|_|*|_|*|_|*|_|*|_|*| | knownSP | secis_index:1 SeB_blast category:ALI_U |*|_|*|_|*|_|*|_|*|_|*|_|*|_|*|_ _________________________________|______________________|_____________________________________________________________________________ Blastx evalue: 2e-27 Query name: gi|928046123|ref|XP_013872017.1| PREDICTED: selenoprotein O [Austrofundulus limnaeus] Query range: 603-691 (total 691) Target: /data/www-rg/seblastian_results/401670042/tempfiles/temptarget.fasta Chromosome: KV884747.1:subseq_2435036,100000_ Strand: - Positions: -Exon 1 45091 45141 -Exon 2 44623 44841 Redox box: YES Query IAQNAIEAAENGDFSEV <---Intron---> QRVLEVLQKPFSSQPGLELPVWVGEPGTTAQAERDEGEEQQHG-ASASAARTPLPYDSKPPAWAQQI ||||||||||||||||| < 249bp > ||||/||/|||||||||||| |||| |/| ||||||||||| || / || |/|||||||/|||/| Target IAQNAIEAAENGDFSEV QRVLKVLEKPFSSQPGLELPAWVGEGGSTEQAERDEGEEQQQEMASTTGARNPVPYDSKPPSWAQEI agcagaggggaggttgg ccgcagcgactttccgcgccgtgggggaagcggagggggcccgagtaaggaacgctgaaccttgcga tcaactaccaagatcat agttattaactccacgtatccgtgagggcaacagaagaaaaaatccccgcgactcaagacccgcaat ccgtaagatgtcccagc ggccgtagattttgcaggttggggatcccagaggtaaggagagggcacacatttctccgcttgcgac Query CVTUSS |||||| Target CVTUSS tgattt gtcgcc tcaatc * Sequence until next stop: TAA * Distance CDS-SECIS: 451 ; UGA-SECIS: 457 ------- SECIS element -- id:1 -- grade:A ---- Chromosome = KV884747.1:subseq_2435036,100000_ Strand = - Positions = 44171 - 44095 Found by: Infernal (score=21.77) Covels (score=21.96) Stem2 length = 9 Apical loop length = 18 Insertion in stem2 = 0, 0 Free Energy = -8.99 ..(((((((.......(((((((((((((((................)))))))))))))))..........))))))).. model uucggucaugcgUuaAUGAcGgccuggcccuAAAcC-cuuuuuggggcgggccaggcCuGAUGuu-u-uuucaugaccggc ..(((({((.--....{[[{(({(((((((..................)))))))}))}]]}......-.-.))})))).. target UGGGUAGCUA--CUAAUGACGACUCAGACACCAAGUCCAACUGUGACCUGUCUGACUCUGACAAACUG-U-GGGGUGUCAU **** ^^ ****