TBLASTN 2.2.13 [Nov-27-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= SPP00000060_2.0 # Protein # Selenophosphate synthetase 1 (SPS1)
# Human (Homo sapiens)
(321 letters)
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g
enome.fa
14,644 sequences; 2,174,243,242 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|397458686|gb|JH736815.1| Alligator mississippiensis unplaced ... 148 4e-34
gi|397458164|gb|JH737337.1| Alligator mississippiensis unplaced ... 118 4e-25
gi|397459716|gb|JH735785.1| Alligator mississippiensis unplaced ... 34 7.5
>gi|397458686|gb|JH736815.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-9464, whole genome shotgun sequence
Length = 1028860
Score = 148 bits (373), Expect = 4e-34
Identities = 71/72 (98%), Positives = 72/72 (100%)
Frame = -3
Query: 250 TGGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVA 309
+GGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVA
Sbjct: 21416 SGGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVA 21237
Query: 310 TQNVNPTPGATS 321
TQNVNPTPGATS
Sbjct: 21236 TQNVNPTPGATS 21201
Score = 132 bits (331), Expect = 3e-29
Identities = 64/65 (98%), Positives = 64/65 (98%)
Frame = -2
Query: 1 MSTRESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAV 60
MS RESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAV
Sbjct: 48849 MSVRESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAV 48670
Query: 61 MPRLG 65
MPRLG
Sbjct: 48669 MPRLG 48655
Score = 108 bits (270), Expect = 3e-22
Identities = 52/52 (100%), Positives = 52/52 (100%)
Frame = -3
Query: 136 ERDKVMPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIM 187
ERDKVMPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIM
Sbjct: 36545 ERDKVMPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIM 36390
Score = 78.2 bits (191), Expect = 4e-13
Identities = 35/35 (100%), Positives = 35/35 (100%)
Frame = -2
Query: 65 GIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMM 99
GIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMM
Sbjct: 46149 GIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMM 46045
Score = 78.2 bits (191), Expect = 4e-13
Identities = 36/48 (75%), Positives = 41/48 (85%)
Frame = -1
Query: 97 YMMGRIACANVLSDLYAMGVTECDNMLMLLGVSNKMTDRERDKVMPLI 144
+ GR ACANVLSDLYAMGVTECDNMLMLLG+SNKMTDR R V+ ++
Sbjct: 37597 FFQGRXACANVLSDLYAMGVTECDNMLMLLGISNKMTDRVRSVVLAVL 37454
Score = 72.0 bits (175), Expect = 3e-11
Identities = 34/34 (100%), Positives = 34/34 (100%)
Frame = -2
Query: 218 PEKWNKIKLVVTQEDVELAYQEAMMNMARLNRTG 251
PEKWNKIKLVVTQEDVELAYQEAMMNMARLNRTG
Sbjct: 31659 PEKWNKIKLVVTQEDVELAYQEAMMNMARLNRTG 31558
Score = 66.2 bits (160), Expect = 2e-09
Identities = 31/33 (93%), Positives = 31/33 (93%)
Frame = -1
Query: 188 PDNAVPGDVLVLTKPLGTQVAVAVHQWLDIPEK 220
PDNAVPGDVLVLTKPLGTQVAVAVHQWLDI K
Sbjct: 35572 PDNAVPGDVLVLTKPLGTQVAVAVHQWLDIVSK 35474
>gi|397458164|gb|JH737337.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-10337, whole genome shotgun sequence
Length = 421118
Score = 118 bits (295), Expect = 4e-25
Identities = 56/66 (84%), Positives = 60/66 (90%)
Frame = +3
Query: 251 GGLLICLPREQAARFCAEIKSPKYGEGHQAWIIGIVEKGNRTARIIDKPRIIEVAPQVAT 310
GGLLICLPREQAARFCAEIKSPK+GEGHQAWIIGIVEKGN+TARIIDKPRIIEV P+ A
Sbjct: 401160 GGLLICLPREQAARFCAEIKSPKHGEGHQAWIIGIVEKGNQTARIIDKPRIIEVMPRGAA 401339
Query: 311 QNVNPT 316
+PT
Sbjct: 401340 ATASPT 401357
Score = 97.1 bits (240), Expect = 9e-19
Identities = 42/52 (80%), Positives = 50/52 (96%)
Frame = +2
Query: 136 ERDKVMPLIIQGFKDAAEEAGTSVTGGQTVLNPWIVLGGVATTVCQPNEFIM 187
ER+K+MPLII+GF+DAAE+ GTSVTGGQTV+NPWI++GGVAT VCQPNEFIM
Sbjct: 399587 EREKIMPLIIKGFRDAAEDGGTSVTGGQTVMNPWIIVGGVATVVCQPNEFIM 399742
Score = 73.2 bits (178), Expect = 1e-11
Identities = 31/42 (73%), Positives = 37/42 (88%)
Frame = +1
Query: 58 GAVMPRLGIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDPYMM 99
G + +GIGMD+CVIPLRHGGLSLVQTTD+ YP+V+DPYMM
Sbjct: 398122 GCFLSPVGIGMDSCVIPLRHGGLSLVQTTDFFYPLVEDPYMM 398247
Score = 70.1 bits (170), Expect = 1e-10
Identities = 33/38 (86%), Positives = 34/38 (89%)
Frame = +2
Query: 100 GRIACANVLSDLYAMGVTECDNMLMLLGVSNKMTDRER 137
GRIACANVLSDLYAMG+TECDNMLMLL VS KMTD R
Sbjct: 398450 GRIACANVLSDLYAMGITECDNMLMLLSVSQKMTDEVR 398563
Score = 61.6 bits (148), Expect(2) = 3e-20
Identities = 28/34 (82%), Positives = 32/34 (94%)
Frame = +3
Query: 218 PEKWNKIKLVVTQEDVELAYQEAMMNMARLNRTG 251
PE+WNKIKLVV++EDVELAYQEAM +MA LNRTG
Sbjct: 400110 PERWNKIKLVVSKEDVELAYQEAMFSMAMLNRTG 400211
Score = 60.8 bits (146), Expect(2) = 3e-20
Identities = 30/45 (66%), Positives = 30/45 (66%)
Frame = +1
Query: 172 LGGVATTVCQPNEFIMPDNAVPGDVLVLTKPLGTQVAVAVHQWLD 216
L G A PDNAVPGDVLVLTKPLGTQVAV HQWLD
Sbjct: 399892 LAGGARRAAHGRILSRPDNAVPGDVLVLTKPLGTQVAVTAHQWLD 400026
Score = 43.9 bits (102), Expect = 0.009
Identities = 21/39 (53%), Positives = 26/39 (66%)
Frame = +1
Query: 7 FNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESL 45
F PE+ L +RLT F EL+G GCKVPQ+ L +LL L
Sbjct: 397822 FEPEALGLSPGWRLTGFGELRG*GCKVPQETLLRLLAGL 397938
>gi|397459716|gb|JH735785.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-7738, whole genome shotgun sequence
Length = 238855
Score = 34.3 bits (77), Expect = 7.5
Identities = 21/66 (31%), Positives = 28/66 (42%)
Frame = -2
Query: 37 VLQKLLESLQENHFQEDEQFLGAVMPRLGIGMDTCVIPLRHGGLSLVQTTDYIYPIVDDP 96
VL +L +S Q N Q GA + L G V+PL GL L + + P P
Sbjct: 233493 VLPQLGDSAQPNPTLSQPQVAGAALSLLAAGQPQGVLPLCRAGLGLPRAQGLVVPPTAQP 233314
Query: 97 YMMGRI 102
GR+
Sbjct: 233313 LATGRL 233296
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis
/genome.fa
Posted date: Oct 18, 2013 5:53 PM
Number of letters in database: 2,174,243,242
Number of sequences in database: 14,644
Lambda K H
0.319 0.136 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 816,753,165
Number of Sequences: 14644
Number of extensions: 12274786
Number of successful extensions: 43287
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 449
Number of HSP's successfully gapped in prelim test: 330
Number of HSP's that attempted gapping in prelim test: 41324
Number of HSP's gapped (non-prelim): 5993
length of query: 321
length of database: 724,747,747
effective HSP length: 129
effective length of query: 192
effective length of database: 722,858,671
effective search space: 138788864832
effective search space used: 138788864832
frameshift window, decay const: 40, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 76 (33.9 bits)