TBLASTN 2.2.13 [Nov-27-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= SPP00000146_2.0 # Protein # tRNA Sec 1 associated protein 1
(SECp43) # Human (Homo sapiens)
(287 letters)
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g
enome.fa
14,644 sequences; 2,174,243,242 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|397461042|gb|JH734459.1| Alligator mississippiensis unplaced ... 95 3e-18
gi|397462975|gb|JH732526.1| Alligator mississippiensis unplaced ... 65 3e-09
gi|397462604|gb|JH732897.1| Alligator mississippiensis unplaced ... 52 2e-05
gi|397455927|gb|JH739573.1| Alligator mississippiensis unplaced ... 52 3e-05
gi|397463390|gb|JH732111.1| Alligator mississippiensis unplaced ... 40 0.086
gi|397456112|gb|JH739388.1| Alligator mississippiensis unplaced ... 39 0.33
gi|397464397|gb|JH731104.1| Alligator mississippiensis unplaced ... 38 0.43
gi|397459752|gb|JH735749.1| Alligator mississippiensis unplaced ... 37 1.2
gi|397460258|gb|JH735243.1| Alligator mississippiensis unplaced ... 37 1.2
gi|397460909|gb|JH734592.1| Alligator mississippiensis unplaced ... 37 1.2
gi|397462492|gb|JH733009.1| Alligator mississippiensis unplaced ... 36 2.1
gi|397462872|gb|JH732629.1| Alligator mississippiensis unplaced ... 35 3.6
>gi|397461042|gb|JH734459.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-5587, whole genome shotgun sequence
Length = 766029
Score = 95.1 bits (235), Expect = 3e-18
Identities = 44/45 (97%), Positives = 45/45 (100%)
Frame = -2
Query: 93 SPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQTGVSK 137
SPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQ+GVSK
Sbjct: 209468 SPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQSGVSK 209334
Score = 83.2 bits (204), Expect = 1e-14
Identities = 39/41 (95%), Positives = 41/41 (100%)
Frame = -3
Query: 137 KGYGFVKFTDELEQKRALTECQGAVGLGSKPVRLSVAIPKA 177
+GYGFVKFTDELEQKRALTECQGAVGLGSKP+RLSVAIPKA
Sbjct: 207361 RGYGFVKFTDELEQKRALTECQGAVGLGSKPIRLSVAIPKA 207239
Score = 82.4 bits (202), Expect = 2e-14
Identities = 37/45 (82%), Positives = 42/45 (93%)
Frame = -2
Query: 243 DPMPQLDVTEANKEFMEQSEELYDALMDCHWQPLDTVSSEIPAMM 287
DP PQLDV+EANK+F+EQSEELYDAL D HWQPLDTVSSEIPA++
Sbjct: 202529 DPAPQLDVSEANKQFVEQSEELYDALTDSHWQPLDTVSSEIPAIL 202395
Score = 75.5 bits (184), Expect = 2e-12
Identities = 33/36 (91%), Positives = 34/36 (94%)
Frame = -3
Query: 43 IPAGYCFVEFADLATAEKCLHKINGKPLPGATPAKR 78
IPAGYCFVEFADLATAEKCLHKINGKPLPGATP +
Sbjct: 213259 IPAGYCFVEFADLATAEKCLHKINGKPLPGATPVSK 213152
Score = 64.7 bits (156), Expect = 4e-09
Identities = 32/47 (68%), Positives = 37/47 (78%)
Frame = -3
Query: 10 LEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLA 56
LEPYMDENF+SRAFATMGE V+SVKIIRNRLTG + F D++
Sbjct: 214444 LEPYMDENFVSRAFATMGELVLSVKIIRNRLTG*ASARASRXFEDVS 214304
Score = 40.4 bits (93), Expect = 0.086
Identities = 17/17 (100%), Positives = 17/17 (100%)
Frame = -1
Query: 76 AKRFKLNYATYGKQPDN 92
AKRFKLNYATYGKQPDN
Sbjct: 210696 AKRFKLNYATYGKQPDN 210646
>gi|397462975|gb|JH732526.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-2408, whole genome shotgun sequence
Length = 843303
Score = 65.1 bits (157), Expect = 3e-09
Identities = 31/42 (73%), Positives = 35/42 (83%)
Frame = +1
Query: 243 DPMPQLDVTEANKEFMEQSEELYDALMDCHWQPLDTVSSEIP 284
DP LDV E NK+FME+SEELYDALM+CHWQPLD V+ EIP
Sbjct: 748351 DPQLFLDVDEMNKQFMERSEELYDALMNCHWQPLD-VTCEIP 748473
Score = 58.5 bits (140), Expect = 3e-07
Identities = 25/46 (54%), Positives = 34/46 (73%)
Frame = +2
Query: 94 PEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQTGVSKGY 139
P+YS+FVG+LTP+VDD LY++F+K YPSC K+ D G S+ Y
Sbjct: 742688 PDYSIFVGELTPEVDDFQLYDYFLKRYPSCIDCKIATDLLGYSR*Y 742825
Score = 52.0 bits (123), Expect = 3e-05
Identities = 22/43 (51%), Positives = 31/43 (72%)
Frame = +3
Query: 134 GVSKGYGFVKFTDELEQKRALTECQGAVGLGSKPVRLSVAIPK 176
G+ +GY FV+F ++ +Q RAL +CQ A GLG K +RLS+ I K
Sbjct: 744075 GICRGYAFVRFGEQGDQMRALQDCQNAAGLGGKRIRLSIGISK 744203
>gi|397462604|gb|JH732897.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-3057, whole genome shotgun sequence
Length = 402444
Score = 52.4 bits (124), Expect = 2e-05
Identities = 43/141 (30%), Positives = 69/141 (48%), Gaps = 7/141 (4%)
Frame = -2
Query: 46 GYCFVEF-----ADLATAEKCLHKINGKPLPGATPAKRFKLNYAT-YGKQPDNSPEYSLF 99
GY FVEF AD A + K+ GKP+ ++N A+ + K D ++F
Sbjct: 70856 GYGFVEFLSEEDADYAIKIMNMIKLYGKPI---------RVNKASAHNKNLDVGA--NIF 70710
Query: 100 VGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLD-QTGVSKGYGFVKFTDELEQKRALTECQ 158
+G+L P++D+ +LY+ F + K++ D TG SKGY F+ F + A E
Sbjct: 70709 IGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFA-SFDASDAAIEAM 70533
Query: 159 GAVGLGSKPVRLSVAIPKASR 179
L ++P+ +S A K S+
Sbjct: 70532 NGQYLCNRPITVSYAFKKDSK 70470
Score = 47.8 bits (112), Expect = 5e-04
Identities = 21/70 (30%), Positives = 41/70 (58%)
Frame = -2
Query: 1 MAASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEK 60
+ A++++G+L+P +DE + F+ G + + KI+R+ TG GY F+ FA ++
Sbjct: 70727 VGANIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGNSKGYAFINFASFDASDA 70548
Query: 61 CLHKINGKPL 70
+ +NG+ L
Sbjct: 70547 AIEAMNGQYL 70518
>gi|397455927|gb|JH739573.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-14112, whole genome shotgun sequence
Length = 197727
Score = 52.0 bits (123), Expect = 3e-05
Identities = 29/72 (40%), Positives = 42/72 (58%), Gaps = 1/72 (1%)
Frame = +1
Query: 89 QPDNSPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLDQ-TGVSKGYGFVKFTDE 147
+P ++ +F GDL +V+D +L F + YPS KV+ D+ TG +KGYGFV F D
Sbjct: 16159 RPSTPDDFRIFCGDLGNEVNDDILARAFSR-YPSFLKAKVIRDKRTGKTKGYGFVSFKDP 16335
Query: 148 LEQKRALTECQG 159
+ RA+ E G
Sbjct: 16336 NDYVRAMREMNG 16371
>gi|397463390|gb|JH732111.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-1774, whole genome shotgun sequence
Length = 265296
Score = 40.4 bits (93), Expect = 0.086
Identities = 36/110 (32%), Positives = 50/110 (45%), Gaps = 18/110 (16%)
Frame = +2
Query: 87 GKQPDNSPEY---SLFVGDLTPDVDDGMLYEFFVKVYP--SCRGGKVVLDQTGVSKGYGF 141
G ++P Y SL+VGDL PDV + MLYE F P S R + ++ T S GY +
Sbjct: 155288 GSMNPSAPSYPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMI--TRRSLGYAY 155461
Query: 142 VKFTDELEQKR-----------ALTECQGAVGLGSK--PVRLSVAIPKAS 178
V F + +R A C+ GLG + P R A P+ +
Sbjct: 155462 VNFQQPADGERRAGAMFPSPAPAPACCRARPGLGFRAGPTRPGAAAPRGA 155611
Score = 39.3 bits (90), Expect = 0.19
Identities = 21/69 (30%), Positives = 36/69 (52%)
Frame = +2
Query: 3 ASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEFADLATAEKCL 62
ASL++GDL P + E + F+ G ++S+++ R+ +T GY +V F A E+
Sbjct: 155324 ASLYVGDLHPDVTEAMLYEKFSPAGP-ILSIRVCRDMITRRSLGYAYVNFQQPADGERRA 155500
Query: 63 HKINGKPLP 71
+ P P
Sbjct: 155501 GAMFPSPAP 155527
Score = 35.0 bits (79), Expect = 3.6
Identities = 19/51 (37%), Positives = 32/51 (62%)
Frame = +1
Query: 129 VLDQTGVSKGYGFVKFTDELEQKRALTECQGAVGLGSKPVRLSVAIPKASR 179
V+ + G SKG+GFV F+ E +A+TE G + + +KP+ +++A K R
Sbjct: 171373 VMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRI-VATKPLYVALAQRKEER 171522
>gi|397456112|gb|JH739388.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-13807, whole genome shotgun sequence
Length = 52635
Score = 38.5 bits (88), Expect = 0.33
Identities = 21/51 (41%), Positives = 33/51 (64%)
Frame = -3
Query: 129 VLDQTGVSKGYGFVKFTDELEQKRALTECQGAVGLGSKPVRLSVAIPKASR 179
V+ + G SKG+GFV F+ E +A+TE G + +GSKP+ +++A K R
Sbjct: 40972 VMLEDGRSKGFGFVCFSSPEEATKAVTEMNGRI-VGSKPLYVALAQRKEER 40823
>gi|397464397|gb|JH731104.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-189, whole genome shotgun sequence
Length = 624414
Score = 38.1 bits (87), Expect = 0.43
Identities = 24/74 (32%), Positives = 41/74 (55%), Gaps = 1/74 (1%)
Frame = -1
Query: 2 AASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEF-ADLATAEK 60
+++L++G+L Y E I F+ G+ V + + +++ P G+CFVE+ A A A +
Sbjct: 388248 SSTLYVGNLSFYTTEEQIQELFSKCGD-VKRIVMGLDKIKKTPCGFCFVEYPAAAACAAR 388072
Query: 61 CLHKINGKPLPGAT 74
C PLPGA+
Sbjct: 388071 C-------PLPGAS 388051
>gi|397459752|gb|JH735749.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-7682, whole genome shotgun sequence
Length = 361259
Score = 36.6 bits (83), Expect = 1.2
Identities = 25/85 (29%), Positives = 44/85 (51%), Gaps = 1/85 (1%)
Frame = +1
Query: 96 YSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLD-QTGVSKGYGFVKFTDELEQKRAL 154
+ +FV +L+PD D+ +L++ F + + KV+ D T KG+GFV T+ E A+
Sbjct: 135001 WCIFVYNLSPDSDESVLWQLFGP-FGAVNNVKVIRDFNTNKCKGFGFVTMTNYDEAAMAI 135177
Query: 155 TECQGAVGLGSKPVRLSVAIPKASR 179
G LG + +++S K +
Sbjct: 135178 ASLNG-YRLGDRVLQVSFKTNKTHK 135249
>gi|397460258|gb|JH735243.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-6846, whole genome shotgun sequence
Length = 571208
Score = 36.6 bits (83), Expect = 1.2
Identities = 26/88 (29%), Positives = 45/88 (51%), Gaps = 1/88 (1%)
Frame = -2
Query: 93 SPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLD-QTGVSKGYGFVKFTDELEQK 151
S + +F+ +L D D+G+L++ F + + KV+ D T KG+GFV T+ E
Sbjct: 69811 SSGWCIFIYNLGQDADEGILWQMFGP-FGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAA 69635
Query: 152 RALTECQGAVGLGSKPVRLSVAIPKASR 179
A+ G LG K +++S K+ +
Sbjct: 69634 MAIASLNG-YRLGDKILQVSFKTNKSHK 69554
>gi|397460909|gb|JH734592.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-5802, whole genome shotgun sequence
Length = 288986
Score = 36.6 bits (83), Expect = 1.2
Identities = 25/85 (29%), Positives = 43/85 (50%), Gaps = 1/85 (1%)
Frame = -1
Query: 96 YSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLD-QTGVSKGYGFVKFTDELEQKRAL 154
+ +FV +L PD D+ +L++ F + + KV+ D T KG+GFV T+ E A+
Sbjct: 129890 WCIFVYNLAPDADESILWQMFGP-FGAVTNVKVIRDFNTNKCKGFGFVTMTNYDEAAMAI 129714
Query: 155 TECQGAVGLGSKPVRLSVAIPKASR 179
G LG + +++S K +
Sbjct: 129713 ASLNG-YRLGDRVLQVSFKTNKTHK 129642
>gi|397462492|gb|JH733009.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-3216, whole genome shotgun sequence
Length = 140410
Score = 35.8 bits (81), Expect = 2.1
Identities = 25/60 (41%), Positives = 31/60 (51%), Gaps = 2/60 (3%)
Frame = +3
Query: 94 PEYSLFVGDLTPDVDDGMLYEFFVKVYP--SCRGGKVVLDQTGVSKGYGFVKFTDELEQK 151
P SL+VGDL PDV + MLYE F P S R + V T S GY ++ F + K
Sbjct: 124332 PLASLYVGDLHPDVTEAMLYEKFSPAGPIMSIRVCRDV--ATRRSLGYAYINFQQPADGK 124505
Score = 35.8 bits (81), Expect = 2.1
Identities = 19/51 (37%), Positives = 32/51 (62%)
Frame = +1
Query: 129 VLDQTGVSKGYGFVKFTDELEQKRALTECQGAVGLGSKPVRLSVAIPKASR 179
V+ + G SKG+GFV F+ E +A+TE G + + +KP+ +++A K R
Sbjct: 133732 VMTEGGHSKGFGFVCFSSPEEATKAVTEMNGRI-VSTKPLYVALAQRKEER 133881
Score = 35.0 bits (79), Expect = 3.6
Identities = 17/50 (34%), Positives = 29/50 (58%)
Frame = +3
Query: 3 ASLWMGDLEPYMDENFISRAFATMGETVMSVKIIRNRLTGIPAGYCFVEF 52
ASL++GDL P + E + F+ G +MS+++ R+ T GY ++ F
Sbjct: 124338 ASLYVGDLHPDVTEAMLYEKFSPAG-PIMSIRVCRDVATRRSLGYAYINF 124484
>gi|397462872|gb|JH732629.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-2560, whole genome shotgun sequence
Length = 573371
Score = 35.0 bits (79), Expect = 3.6
Identities = 25/85 (29%), Positives = 43/85 (50%), Gaps = 1/85 (1%)
Frame = +2
Query: 93 SPEYSLFVGDLTPDVDDGMLYEFFVKVYPSCRGGKVVLD-QTGVSKGYGFVKFTDELEQK 151
S + +FV +L P+ D+ +L++ F + + KV+ D T KG+GFV T+ E
Sbjct: 358772 SAGWCIFVYNLAPEADESVLWQLFGP-FGAVTNVKVIRDFATNKCKGFGFVTMTNYEEAA 358948
Query: 152 RALTECQGAVGLGSKPVRLSVAIPK 176
A+ G LG + +++S K
Sbjct: 358949 MAIAALNG-YRLGDRVLQVSFKTSK 359020
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis
/genome.fa
Posted date: Oct 18, 2013 5:53 PM
Number of letters in database: 2,174,243,242
Number of sequences in database: 14,644
Lambda K H
0.317 0.135 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 623,425,131
Number of Sequences: 14644
Number of extensions: 7795212
Number of successful extensions: 26873
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 232
Number of HSP's successfully gapped in prelim test: 143
Number of HSP's that attempted gapping in prelim test: 26132
Number of HSP's gapped (non-prelim): 2275
length of query: 287
length of database: 724,747,747
effective HSP length: 128
effective length of query: 159
effective length of database: 722,873,315
effective search space: 114936857085
effective search space used: 114936857085
frameshift window, decay const: 40, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 75 (33.5 bits)