TBLASTN 2.2.13 [Nov-27-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= SPP00000026_2.0 # Protein # Glutathione peroxidase 4 (GPx4) #
Human (Homo sapiens)
(197 letters)
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis/g
enome.fa
14,644 sequences; 2,174,243,242 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|397462837|gb|JH732664.1| Alligator mississippiensis unplaced ... 121 4e-43
gi|397458980|gb|JH736521.1| Alligator mississippiensis unplaced ... 87 4e-16
gi|397460984|gb|JH734517.1| Alligator mississippiensis unplaced ... 78 2e-13
gi|397461646|gb|JH733855.1| Alligator mississippiensis unplaced ... 70 6e-11
gi|397459705|gb|JH735796.1| Alligator mississippiensis unplaced ... 62 1e-08
gi|397464367|gb|JH731134.1| Alligator mississippiensis unplaced ... 58 2e-07
gi|397463514|gb|JH731987.1| Alligator mississippiensis unplaced ... 42 0.018
gi|397460676|gb|JH734825.1| Alligator mississippiensis unplaced ... 33 8.4
>gi|397462837|gb|JH732664.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-2624, whole genome shotgun sequence
Length = 587981
Score = 121 bits (303), Expect(2) = 4e-43
Identities = 57/101 (56%), Positives = 75/101 (74%)
Frame = -1
Query: 29 CASRDDWRCARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQXGKTEVNYTQLVDLHA 88
CA D W +S++E KDIDG V+L+KY G+ CI+TN+AS+ GKT++NYTQLVD++A
Sbjct: 271838 CAQVDYWCSTKSIYESHVKDIDGSHVSLEKYWGYACIITNIASK*GKTDINYTQLVDMYA 271659
Query: 89 RYAECGLRILAFPCNQFGKQEPGSNEEIKEFAAGYNVKFDM 129
E LRILAFPCNQF KQ PG+N +IK F A Y +K+D+
Sbjct: 271658 I*TERCLRILAFPCNQFKKQ*PGNNAQIKAFVASYGMKYDI 271536
Score = 76.6 bits (187), Expect(2) = 4e-43
Identities = 34/53 (64%), Positives = 40/53 (75%)
Frame = -2
Query: 130 FSKICVNGDDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLIDKNGCVVKRYGP 182
FSK V GD+ PLWKW++ QPKG+G LGNAIKWNFTKFLID G + + GP
Sbjct: 271531 FSKTDVYGDNTLPLWKWLQEQPKGRGTLGNAIKWNFTKFLIDNEGSLWDQEGP 271373
>gi|397458980|gb|JH736521.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-8967, whole genome shotgun sequence
Length = 861763
Score = 87.0 bits (214), Expect = 4e-16
Identities = 41/53 (77%), Positives = 47/53 (88%)
Frame = -1
Query: 60 RGFVCIVTNVASQXGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGS 112
RG VCI+TNVAS+ GKT+VNYTQ VD++ARYAE GLRILAFPCNQFGKQ G+
Sbjct: 287899 RGQVCIITNVASK*GKTDVNYTQFVDMYARYAERGLRILAFPCNQFGKQVGGA 287741
Score = 81.6 bits (200), Expect = 2e-14
Identities = 37/51 (72%), Positives = 40/51 (78%)
Frame = -3
Query: 108 QEPGSNEEIKEFAAGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGILG 158
QEPG N +IK FA Y VKFDMFSKI VNGD A PLWKW+K QPKG+G LG
Sbjct: 287198 QEPGDNAQIKAFAESYGVKFDMFSKIDVNGDHALPLWKWLKEQPKGRGTLG 287046
Score = 33.5 bits (75), Expect = 4.9
Identities = 14/22 (63%), Positives = 18/22 (81%)
Frame = -3
Query: 167 KFLIDKNGCVVKRYGPMEEPLV 188
+FLID+ G VVKRY PME+ +V
Sbjct: 286439 QFLIDRQGQVVKRYSPMEDXIV 286374
>gi|397460984|gb|JH734517.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-5685, whole genome shotgun sequence
Length = 643167
Score = 78.2 bits (191), Expect = 2e-13
Identities = 38/84 (45%), Positives = 53/84 (63%), Gaps = 1/84 (1%)
Frame = -3
Query: 65 IVTNVASQXGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFAAG-Y 123
+V NVAS T+ NY L +LH + +LAFPCNQFG+ EP S++EI+ FA G Y
Sbjct: 563938 LVVNVASYCQHTDKNYIALQELHREFGPSHFTVLAFPCNQFGESEPSSSQEIESFAKGNY 563759
Query: 124 NVKFDMFSKICVNGDDAHPLWKWM 147
V F +F KI + G +A P +K++
Sbjct: 563758 GVTFPIFHKIKILGSEAEPAFKFL 563687
>gi|397461646|gb|JH733855.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-4572, whole genome shotgun sequence
Length = 115602
Score = 69.7 bits (169), Expect = 6e-11
Identities = 36/86 (41%), Positives = 52/86 (60%), Gaps = 1/86 (1%)
Frame = +2
Query: 63 VCIVTNVASQXGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFA-A 121
V +V NVAS+ G T+ +Y L L +LAFPCNQFG+QEP +++EI+ FA
Sbjct: 12224 VSLVVNVASECGYTDSHYKALQQLQRDLGPYHFNVLAFPCNQFGQQEPDTDKEIENFARK 12403
Query: 122 GYNVKFDMFSKICVNGDDAHPLWKWM 147
Y F +FSK V+G A+ +K++
Sbjct: 12404 TYGATFPLFSKTAVSGAGANAAFKYL 12481
>gi|397459705|gb|JH735796.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-7762, whole genome shotgun sequence
Length = 1008463
Score = 62.0 bits (149), Expect = 1e-08
Identities = 34/74 (45%), Positives = 45/74 (60%)
Frame = -3
Query: 38 ARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQXGKTEVNYTQLVDLHARYAECGLRI 97
A+S ++ SA + G V+ + +RG V ++ NVAS G T +YTQL L ARY L +
Sbjct: 892055 AKSFYDLSAITLQGEKVDFNNFRGRVVLIENVASL*GTTVRDYTQLNQLQARYPR-RLVV 891879
Query: 98 LAFPCNQFGKQEPG 111
L FPCNQFG Q G
Sbjct: 891878 LGFPCNQFGYQVSG 891837
Score = 46.2 bits (108), Expect = 7e-04
Identities = 36/129 (27%), Positives = 54/129 (41%), Gaps = 23/129 (17%)
Frame = -3
Query: 88 ARYAECGLRILAFPCNQFGKQEPGSNEEIKEF------AAGYNVKFDMFSKICVNGDDAH 141
AR+ G+ +L+ P QE +NEEI G+ F +F K VNG D H
Sbjct: 890471 ARHPAQGVFVLSCPL-----QENCTNEEILNSLKYVRPGGGFEPNFTLFQKCQVNGQDTH 890307
Query: 142 PLWKWMK--------------IQPK---GKGILGNAIKWNFTKFLIDKNGCVVKRYGPME 184
P++ ++K P+ + + WNF KFL+ G KRY P
Sbjct: 890306 PVFAYLKAHLPAPVDNPAALLTDPRFLVWSPVQRADLTWNFEKFLVGPEGEPFKRYSPKF 890127
Query: 185 EPLVIEKDL 193
+ +E D+
Sbjct: 890126 QTSHVEPDI 890100
>gi|397464367|gb|JH731134.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-234, whole genome shotgun sequence
Length = 432364
Score = 57.8 bits (138), Expect = 2e-07
Identities = 30/62 (48%), Positives = 38/62 (61%)
Frame = +3
Query: 51 GHMVNLDKYRGFVCIVTNVASQXGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEP 110
G + L G V +V+NVAS G T ++TQL +L RY GL +L FPCNQFG Q P
Sbjct: 173997 GEPLALGSLLGRVLLVSNVASL*GTTARDFTQLNELQERYGPRGLLVLGFPCNQFGHQVP 174176
Query: 111 GS 112
G+
Sbjct: 174177 GA 174182
Score = 49.3 bits (116), Expect = 9e-05
Identities = 31/89 (34%), Positives = 41/89 (46%), Gaps = 17/89 (19%)
Frame = +1
Query: 122 GYNVKFDMFSKICVNGDDAHPLWKWMK--------------IQPK---GKGILGNAIKWN 164
GY F MF K VNG +AHPL+ ++K P+ + N I WN
Sbjct: 177016 GYEPNFTMFEKCDVNGANAHPLFTFLKEALPFPHDDPVALMTNPQYIIWSPVCRNDISWN 177195
Query: 165 FTKFLIDKNGCVVKRYGPMEEPLVIEKDL 193
F KFLI +G KRY E + I+ D+
Sbjct: 177196 FEKFLIGPDGIPFKRYSRRFETIKIQDDI 177282
>gi|397463514|gb|JH731987.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-1586, whole genome shotgun sequence
Length = 466719
Score = 41.6 bits (96), Expect = 0.018
Identities = 20/37 (54%), Positives = 23/37 (62%)
Frame = +1
Query: 80 YTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEI 116
+ +L L + GL IL FP NQFGKQEPG N EI
Sbjct: 209929 HAELNALQSELGPYGLVILGFPSNQFGKQEPGQNAEI 210039
>gi|397460676|gb|JH734825.1| Alligator mississippiensis unplaced genomic
scaffold scaffold-6195, whole genome shotgun sequence
Length = 492535
Score = 32.7 bits (73), Expect = 8.4
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = +2
Query: 59 YRGFVCIVTNVASQXGKTEVNYTQLVDLHARYAE 92
Y F+C+ V GK +V+YT VDL R+ E
Sbjct: 15992 YYSFMCVYDEV*GNVGKIDVHYTSSVDLKVRFRE 16093
Database: /cursos/BI/genomes/project_2014/Alligator_mississippiensis
/genome.fa
Posted date: Oct 18, 2013 5:53 PM
Number of letters in database: 2,174,243,242
Number of sequences in database: 14,644
Lambda K H
0.324 0.140 0.457
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 572,287,233
Number of Sequences: 14644
Number of extensions: 8453572
Number of successful extensions: 27620
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 485
Number of HSP's successfully gapped in prelim test: 261
Number of HSP's that attempted gapping in prelim test: 26243
Number of HSP's gapped (non-prelim): 3976
length of query: 197
length of database: 724,747,747
effective HSP length: 123
effective length of query: 74
effective length of database: 722,946,535
effective search space: 53498043590
effective search space used: 53498043590
frameshift window, decay const: 40, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 72 (32.3 bits)