SELENOPROTEÍNAS DE Meleagris gallopavo
PROTEIN REPORT FOR Selenoprotein 15 (Sel 15)
| DESCRIPCIÓN | Selenoproteína. 10(+):16641642...16657948. |
| SECISearch | Sin resultados. |
| SELENOPROFILES | Encontrada. Elección: Genewise. Predicción elemento SECIS (strand:+ positions:16658472-16658570) |
| COMENTARIOS | La homología con la Sel 15 parece clara, con presencia de Selenocisteína y no de cisteína (existen homólogos con cisteína en otras especies). |
1. ALINEAMIENTO MÚLTIPLE DE TODOS LOS HOMÓLOGOS DE SELENODB
2. BEST PAIRWISE ALIGNMENT
4. RESULTADOS DEL Selenoprofiles
ALINEAMIENTO MÚLTIPLE DE TODOS LOS HOMÓLOGOS DE SELENODB
CLUSTAL FORMAT for T-COFFEE Version_7.54, SCORE=80, Nseq=15, Len=196
SPP00000013_1.0 MVAMAAGPSGCLVPAFGLRLLLATVLQAVSAFGAEFSSEACRELGF-SSNLLCSSCDLLG
SPP00000013_1.0.10.exonerate VRC-APCPAGSRVPGFLLKMKYTKFTSQINVYGAQLSSEACRELGF-SSNLLCSSCNLLG
SPP00000013_1.0.10.genewise ----------------------------INVYGAQLSSEACRELGF-SSNLLCSSCNLLG
SPP00000064_1.0 MSG------------EVYLLWLLSLLKTLSAYGAELSSEACRELGF-SSNLLCTSCDLLG
SPP00000064_1.0.10.exonerate ----------------------------INVYGAQLSSEACRELGF-SSNLLCSSCNLLG
SPP00000064_1.0.10.genewise ----------------------------INVYGAQLSSEACRELGF-SSNLLCSSCNLLG
SPP00000109_1.0 MAA---GQGGWLRPALGLRLLLATAFQAASALGAEFASEACRELGF-SSNLLCSSCDLLG
SPP00000109_1.0.10.exonerate -----------------------------NVYGAQLSSEACRELGF-SSNLLCSSCNLLG
SPP00000109_1.0.10.genewise --------------------------------GAQLSSEACRELGF-SSNLLCSSCNLLG
SPP00000040_1.0 MHK------------CAIF---LLLALSCQQIQAELTAADCRALGFIKAQLMCSSCEKLD
SPP00000040_1.0.10.exonerate ---------------------------------AQLSSEACRELGF-SSNLLCSSCNLLG
SPP00000040_1.0.10.genewise ---------------------------------AQLSSEACRELGF-SSNLLCSSCNLLG
SPP00000051_1.0 MRL------------FAIT---CLLFSIVTVIGAEFSAEDCRELGLIKSQLFCSSCSSLS
SPP00000051_1.0.10.exonerate ----------------------CCIDLQINVYGAQLSSEACRELGF-SSNLLCSSCNLLG
SPP00000051_1.0.10.genewise ----------------------CCIDLQINVYGAQLSSEACRELGF-SSNLLCSSCNLLG
*:::: ** **: .::*:*:**. *.
SPP00000013_1.0 QFNLLQLDPDCRGCCQEEAQFETK-K-LYAGAILEVCGUKLGRFPQVQV-----------
SPP00000013_1.0.10.exonerate QFNLNQLDPFCRQCCQEEAQLETR-K-LYAGAVLEVCGUKLGRFPQVQVCA---------
SPP00000013_1.0.10.genewise QFNLNQLDPFCRQCCQEEAQLETR-K-LYAGAVLEVCGUKLGRFPQVQVCA---------
SPP00000064_1.0 EFSLAKLQPNCQQCCQQEALMEGR-K-LYPGAILEVCGUKLGRFPQVQAFVRSEKPKMFK
SPP00000064_1.0.10.exonerate QFNLNQLDPFCRQCCQEEAQLETR-K-LYAGAVLEVCGUKLGRFPQVQAFVRSDKPKLFR
SPP00000064_1.0.10.genewise QFNLNQLDPFCRQCCQEEAQLETR-K-LYAGAVLEVCGUKLGRFPQVQAFVRSDKPKLFR
SPP00000109_1.0 QFNLLPLDPVCRGCCQEEAQFETK-K-LYAGAILEVCGUKLGRFPQVQAFVRSDKPKLFR
SPP00000109_1.0.10.exonerate QFNLNQLDPFCRQCCQEEAQLETR-K-LYAGAVLEVCGUKLGRFPQVQAFVRSDKPKLFR
SPP00000109_1.0.10.genewise QFNLNQLDPFCRQCCQEEAQLETR-K-LYAGAVLEVCGUKLGRFPQVQAFVRSDKPKLFR
SPP00000040_1.0 DFGLDTIKPQCKQCCTLDQQPAAQ-R-TYAKAILEVCTCKFRAYPQIQAFIQSGRPAKFP
SPP00000040_1.0.10.exonerate QFNLNQLDPFCRQCCQEEAQLETR-K-LYAGAVLEVCGUKLGRFPQVQAFVRSDKPKLFR
SPP00000040_1.0.10.genewise QFNLNQLDPFCRQCCQEEAQLETR-K-LYAGAVLEVCGUKLGRFPQVQAFVRSDKPKLFR
SPP00000051_1.0 DYGLIELKEHCLECCQKDTEADSKLK-VYPAAVLEVCTCKFGAYPQIQAFIKSDRPAKFP
SPP00000051_1.0.10.exonerate QFNLNQLDPFCRQCCQEEAQLETR-KQLYAGAVLEVCGUKLGRFPQVQAFVRSDKPKLFR
SPP00000051_1.0.10.genewise QFNLNQLDPFCRQCCQEEAQLETR-K-LYAGAVLEVCGUKLGRFPQVQAFVRSDKPKLFR
::.* :. * ** : : : *. *:**** *: :**:*.
SPP00000013_1.0 -----------------------------CPWF-RPCIKAF-GRQW--------------
SPP00000013_1.0.10.exonerate -------------------------------WF-P--CTEAAGRQW--------------
SPP00000013_1.0.10.genewise -------------------------------WFXP--CTEAAGRQW--------------
SPP00000064_1.0 GLQIKYVRGSDPVLKLLDDEGNIAEELSILKWN-TDSVEEFLSEKL--------------
SPP00000064_1.0.10.exonerate GLQIKYVRGSDPVLKLLDDSGNIAEELSILKWN-TDSVEEFLSEKL--------------
SPP00000064_1.0.10.genewise GLQIKYVRGSDPVLKLLDDSGNIAEELSILKWN-TDSVEEFLSEKL--------------
SPP00000109_1.0 GLQIKYVRGSDPVLKLLDDNGNIAEELSILKWN-TDSVEEFLSEKL--------------
SPP00000109_1.0.10.exonerate GLQIKYVRGSDPVLKLLDDSGNIAEELSILKWN-TDSVEEFLSEKL--------------
SPP00000109_1.0.10.genewise GLQIKYVRGSDPVLKLLDDSGNIAEELSILKWN-TDSVEEFLSEKL--------------
SPP00000040_1.0 NLQIKYVRGLDPVVKLLDASGKVQETLSITKWN-TDTVEEFFETHLAKDGAGKNSYSVVE
SPP00000040_1.0.10.exonerate GLQIKYVRGSDPVLKLLDDSGNIAEELSILKWN-TDSVEEFLSEKL--------------
SPP00000040_1.0.10.genewise GLQIKYVRGSDPVLKLLDDSGNIAEELSILKWN-TDSVEEFLSEKL--------------
SPP00000051_1.0 NLTIKYVRGLDPIVKLMDEQGNVKETLSINKWN-TDTVQEFFETRLAKV-----------
SPP00000051_1.0.10.exonerate GLQIKYVRGSDPVLKLLDDSGNIAEELSILKWN-TDSVEEFLSEKL--------------
SPP00000051_1.0.10.genewise GLQIKYVRGSDPVLKLLDDSGNIAEELSILKWN-TDSVEEFLSEKL--------------
* :
SPP00000013_1.0 -------------EHC
SPP00000013_1.0.10.exonerate -------------EHC
SPP00000013_1.0.10.genewise -------------EHC
SPP00000064_1.0 -------------DRI
SPP00000064_1.0.10.exonerate -------------ERL
SPP00000064_1.0.10.genewise -------------ERL
SPP00000109_1.0 -------------ERI
SPP00000109_1.0.10.exonerate -------------ERL
SPP00000109_1.0.10.genewise -------------ERL
SPP00000040_1.0 DADGDDDEDYLRTNRI
SPP00000040_1.0.10.exonerate -------------E-R
SPP00000040_1.0.10.genewise -------------E-R
SPP00000051_1.0 -----EDDDYLKTNRV
SPP00000051_1.0.10.exonerate -------------ERL
SPP00000051_1.0.10.genewise -------------ERL
:
......................................................................................................................................................................................................................................................
BEST PAIRWISE ALIGNMENT
CLUSTAL FORMAT for T-COFFEE Version_7.54 , SCORE=100, Nseq=2, Len=162
SPP00000109_1.0 MAAGQGGWLRPALGLRLLLATAFQAASALGAEFASEACRELGFSSNLLCSSCDLLGQFNL
SPP00000109_1.0.10.genewise -----------------------------GAQLSSEACRELGFSSNLLCSSCNLLGQFNL
**:::******************:*******
SPP00000109_1.0 LPLDPVCRGCCQEEAQFETKKLYAGAILEVCGUKLGRFPQVQAFVRSDKPKLFRGLQIKY
SPP00000109_1.0.10.genewise NQLDPFCRQCCQEEAQLETRKLYAGAVLEVCGUKLGRFPQVQAFVRSDKPKLFRGLQIKY
***.** *******:**:******:***** ***************************
SPP00000109_1.0 VRGSDPVLKLLDDNGNIAEELSILKWNTDSVEEFLSEKLERI
SPP00000109_1.0.10.genewise VRGSDPVLKLLDDSGNIAEELSILKWNTDSVEEFLSEKLERL
*************.***************************:
......................................................................................................................................................................................................................................................
RESULTADOS DEL Selenoprofiles
Output_id: Sel15.1.selenocysteine
---------- ----------------------
-Species Meleagris gallopavo -Taxid 9103
-Target /homes/users/U63748/gallopavo.fa
-Chromosome (+) 10
-Program genewise
-Query name gi|144953871|ref|NP_001029931.2| 15 kDa selenoprotein [Bos taurus] gi|172048656|sp|A8YXY3.2|SEP15_BOVIN
RecName: Full=15 kDa selenoprotein_ Flags: Precursor gi|158454994|gb|AAI02887.1| 15 kDa selenoprotein [Bos taurus]
-Query range 26-162 length:162 coverage: 0.85
-Profile range 33-233 length:233 coverage: 0.86 sec_position: [106]
-Average sequence identity with profile: 0.3875 (ignoring gaps: 0.4977)
-State kept
------- alignment -------
Query VSALGAEFSSESCRELGFSSNLLCSSCDLLGQFNLLQLDPDCRGCCQEEAQFETKK <---Intron---> LYAGAILEVCGUKLGRFPQVQA <---I
// ||/ |||/|||||||||||||||/||||||| |||| || ||||||| ||/| < 6968bp > |||||/|||||||||||||||| < 6
Target INVYGAQLSSEACRELGFSSNLLCSSCNLLGQFNLNQLDPFCRQCCQEEAQLETRK LYAGAVLEVCGUKLGRFPQVQA
aagtggccttggtagcgttaatctattaccgctacactgcttacttcgggccgaaa ctggggcggtgtatgatccgcg
tatagcatccacggatgtcgattggcgattgatataatactggaggaaacatacga tacgcttatgggatggtcata
acataaggaaatcgattcctcgccctccttagctgtggtactggctaaatggacag ttaatcagataaagcgctacg
*
Query ntron---> FVRSDKPKLFKGLQIK <---Intron---> YVRGSDPVLKLLDDSGNIAEELSILKWNTDSVEEFLSEKLERI
964bp > ||||||||||/||||| < 1976bp > ||||||||||||||||||||||||||||||||||||||||||/
Target FVRSDKPKLFRGLQIK YVRGSDPVLKLLDDSGNIAEELSILKWNTDSVEEFLSEKLERL
tgaagacactagccaa tgcgtgcgcaccggagaagggcaacataagagggtcagatgcc
cttggaacattggtata atggcacttattaaggatcaatgttagacagtaattgaatagt
ttcgttgtgacgagacg tgtttctaggggcctgctaaagcccggtatcgagcataaagta
------- positions -------
Exon 1 16641630 16641797
Exon 2 16648766 16648829
Exon 3 16655794 16655843
Exon 4 16657820 16657948
--------- SECIS ---------
>Sel15.1.selenocysteine.esecis:def.1 chromosome:10 strand:+ positions:16658472-16658570 species:"Meleagris gallopavo"
target:/homes/users/U63748/gallopavo.fa distance_from_sec_uga:730 distance_from_cds:523
UGCAAAACAU UUGGUAAG CACACUUUA GUGAA GACUGCAUAGA AA ACCUCUGAGG UAUGUGCUAGUC AGAU GUCAGGUGUG CUUUGCAU CAAGUGUUUU
...((((((( (((((((( (((((((.( .(((( (((((((((.. .. ((((...))) ).))))).)))) )))) .).))))))) )))...)) ))))))))))
--------- 3' seq --------
Total sequence length available downstream >= 3000
Sequence until first stop codon:
TAA
*
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