TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000027_1.0 # Protein # Selenoprotein U2 (SelU2) # Homo sapiens # Complete (226 letters) Database: I.multifiliis_strain_G5/genome.fa 2017 sequences; 48,799,969 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|340502181|gb|GL984204.1| Ichthyophthirius multifiliis unplace... 64 1e-10 >gi|340502181|gb|GL984204.1| Ichthyophthirius multifiliis unplaced genomic scaffold scaff_1120509251243, whole genome shotgun sequence Length = 165186 Score = 64.3 bits (155), Expect = 1e-10 Identities = 44/150 (29%), Positives = 75/150 (50%), Gaps = 4/150 (2%) Frame = -1 Query: 69 YICKEYVEDLAKIPRSFLQEANVTLIVIGQSSYHHIEPFCKLTGY-SHEIYVDPEREIYK 127 ++C++++ +++I + L NV L VIG I FC TG+ ++ +VDPER IY Sbjct: 81930 FVCRDFIRSISRISWNIL**KNVDLKVIGCGKTSGINSFCVDTGFLPNKCFVDPERVIY* 81751 Query: 128 RLGMKRGEE---IASSGQSPHIKSNLLSGSLQSLWRAVTGPLFDFQGDPAQQGGTLILGP 184 RLG+ R E+ + S + L G LW ++ GD GG+++ GP Sbjct: 81750 RLGLTRVEKPSGLIGKN*SAETTTGLCCG---FLWTSIAFCRRGV*GDVF*LGGSILAGP 81580 Query: 185 GNNIHFIHRDRNRLDHKPINSVLQLVGVQH 214 G F D + DH ++++L+G+ + Sbjct: 81579 G*YFFFKFNDESPEDHISYVAMIELLGISY 81490 Database: I.multifiliis_strain_G5/genome.fa Posted date: Nov 21, 2011 7:46 PM Number of letters in database: 48,799,969 Number of sequences in database: 2017 Lambda K H 0.319 0.137 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,376,461 Number of Sequences: 2017 Number of extensions: 82173 Number of successful extensions: 132 Number of sequences better than 1.0e-04: 1 Number of HSP's better than 0.0 without gapping: 15 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 114 Number of HSP's gapped (non-prelim): 19 length of query: 226 length of database: 16,266,656 effective HSP length: 99 effective length of query: 127 effective length of database: 16,066,973 effective search space: 2040505571 effective search space used: 2040505571 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 103 (44.3 bits)