TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Sps2.Perkinsus_marinus located on
gi|126302146|gb|AAXJ01000359.1|:subseq(27302,5000) Perkinsus
marinus:[translate(1)] #elongated from blast
         (330 letters)

Database: I.multifiliis_strain_G5/genome.fa 
           2017 sequences; 48,799,969 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|340501239|gb|GL984296.1| Ichthyophthirius multifiliis unplace...   102   6e-47

>gi|340501239|gb|GL984296.1| Ichthyophthirius multifiliis unplaced
             genomic scaffold scaff_1120509251335, whole genome shotgun
             sequence
          Length = 70427

 Score =  102 bits (253), Expect(3) = 6e-47
 Identities = 51/123 (41%), Positives = 80/123 (65%), Gaps = 1/123 (0%)
 Frame = -3

Query: 1     VWDPTSLGLSKDFVLTSFSERKGXGCKVQPDQLSSFLPQIGDGNMLTEDSPDCSMVPVGD 60
             +W+PT   L K FVLT +S+ KG GCKV    L  +L ++G+   +T ++PD S+ PV +
Sbjct: 41844 LWNPTDHNLEKTFVLTDYSDLKG*GCKVPQADLKKYLEKLGNAK*ITSEAPDVSVRPVRN 41665

Query: 61    GE-RVLVSSTDFLSPISQDPFHQGWIAAANTLSDLYAVGADDINNILMILTVSMKMSPLE 119
              + + L+S+ DF  P+ +DP+  G IA  N LSDL+++G  DI+ +LM+L VS KM+  +
Sbjct: 41664 TKTKKLISTIDFFYPLVEDPYM*GRIACCNVLSDLFSMGVTDIDTVLMVLAVSNKMNA*Q 41485

Query: 120   QTV 122
             + V
Sbjct: 41484 KEV 41476



 Score = 65.9 bits (159), Expect(3) = 6e-47
 Identities = 37/114 (32%), Positives = 61/114 (53%), Gaps = 6/114 (5%)
 Frame = -2

Query: 122   VTTKKMIEGFNACCEEAGTKVTGGQTVFNPWPLLGGCAMATMLQSQLVTTDRAVPGNVIV 181
             + T  MI GF+ C    G  V GG T+ NPWP++GG  ++ + + + +  + A  G++I+
Sbjct: 41425 IVTSLMI*GFDECA**VGAYVLGG*TIINPWPIIGGVGISVVEREEFLMPNNAKVGDMII 41246

Query: 182   LTKPLGTQVTGNLALWMMNEAQ------WGKVVKAGLDVETCTRAVKMGDLSMM 229
             LTKPLGT +  NL  W   + +       G + K  +D       ++MG LS++
Sbjct: 41245 LTKPLGT*LAVNLK*WYTKDIKDKLEKI*GFISKE*VD*AFLKAEMEMGTLSIL 41084



 Score = 59.7 bits (143), Expect(3) = 6e-47
 Identities = 36/91 (39%), Positives = 56/91 (61%)
 Frame = -1

Query: 238   LMREFGALGATDVTGFGLVGHADNLARVQKSGASIEIDTVPTIQGCKTLSMVRPNGRDYG 297
             LM+++ A   TDVTGFG+ GH+  LA V K    + I+ +PTI   K ++ +    RD+ 
Sbjct: 41075 LMKKYKANACTDVTGFGIKGHS*FLAEV*KE*VDLIINNLPTI---KYVASIDGKARDFK 40905

Query: 298   LLEGRAAETSGGLLVMLRDKETAEKFIAALR 328
                G + ETSGGLL+ L +K  A++FI+ ++
Sbjct: 40904 FK*GLSPETSGGLLICL-EKNKAKEFISEMK 40815


  Database: I.multifiliis_strain_G5/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 48,799,969
  Number of sequences in database:  2017
  
Lambda     K      H
   0.318    0.134    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,188,710
Number of Sequences: 2017
Number of extensions: 46496
Number of successful extensions: 70
Number of sequences better than 1.0e-04: 1
Number of HSP's better than  0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 62
Number of HSP's gapped (non-prelim): 7
length of query: 330
length of database: 16,266,656
effective HSP length: 103
effective length of query: 227
effective length of database: 16,058,905
effective search space: 3645371435
effective search space used: 3645371435
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 105 (45.1 bits)