TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Sps2.Perkinsus_marinus located on
gi|126302146|gb|AAXJ01000359.1|:subseq(27302,5000) Perkinsus
marinus:[translate(1)] #elongated from blast
         (330 letters)

Database: D.discoideum_AX4/genome.fa 
           6 sequences; 33,943,072 total letters

Searching......done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|93569092|gb|CM000152.2| Dictyostelium discoideum AX4 chromoso...   203   3e-56

>gi|93569092|gb|CM000152.2| Dictyostelium discoideum AX4 chromosome 3, whole
               genome shotgun sequence
          Length = 6357299

 Score =  203 bits (516), Expect(2) = 3e-56
 Identities = 112/253 (44%), Positives = 157/253 (62%)
 Frame = +3

Query: 71      FLSPISQDPFHQGWIAAANTLSDLYAVGADDINNILMILTVSMKMSPLEQTVTTKKMIEG 130
               F  P+  DP+ QG IA AN LSDLY+ G +D +N+LM+L  S  M+  ++  ++K MI+G
Sbjct: 5741658 FFFPLVDDPYFQGKIACANVLSDLYSFGIEDCDNMLMLLACSTDMTAEQRQWSSKLMIQG 5741837

Query: 131     FNACCEEAGTKVTGGQTVFNPWPLLGGCAMATMLQSQLVTTDRAVPGNVIVLTKPLGTQV 190
               FN     AG+KV+GGQTV NPWP++GG A + +  ++ +    AVPG+V+VLTKPLGTQV
Sbjct: 5741838 FNDQAICAGSKVSGGQTVKNPWPIVGGVATSILKTNEFIKPVNAVPGDVLVLTKPLGTQV 5742017

Query: 191     TGNLALWMMNEAQWGKVVKAGLDVETCTRAVKMGDLSMMTLNKNGCRLMREFGALGATDV 250
                 N   W+    +W K +    + E C +      LSM  LN+ G RLM+++ A  ATDV
Sbjct: 5742018 CVNFHQWLSKPERWEK-INTITNAEECEQVFNYATLSMARLNRVGARLMKKYNAHAATDV 5742194

Query: 251     TGFGLVGHADNLARVQKSGASIEIDTVPTIQGCKTLSMVRPNGRDYGLLEGRAAETSGGL 310
               TGFG++GH+ NLA+ Q      EI T+P I+  K L         + LL+G +AETSGGL
Sbjct: 5742195 TGFGILGHSTNLAQNQLLPIRFEIHTLPIIKHMKKLE--DHLNHPFKLLKGTSAETSGGL 5742368

Query: 311     LVMLRDKETAEKF 323
               L+ +  +E AE F
Sbjct: 5742369 LISM-SRENAEAF 5742404



 Score = 34.3 bits (77), Expect(2) = 3e-56
 Identities = 21/56 (37%), Positives = 30/56 (53%)
 Frame = +1

Query: 15      LTSFSERKGXGCKVQPDQLSSFLPQIGDGNMLTEDSPDCSMVPVGDGERVLVSSTD 70
               LT F++ KG GCKV   +L S L  IG+G        DCS+      + V++ +TD
Sbjct: 5741401 LTDFTKLKG*GCKVPQAELLSLLDGIGEG-----IGYDCSISQTKFPDIVMIQTTD 5741553


  Database: D.discoideum_AX4/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 33,943,072
  Number of sequences in database:  6
  
Lambda     K      H
   0.318    0.134    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,125,188
Number of Sequences: 6
Number of extensions: 67554
Number of successful extensions: 242
Number of sequences better than 1.0e-04: 1
Number of HSP's better than  0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 221
Number of HSP's gapped (non-prelim): 27
length of query: 330
length of database: 11,314,357
effective HSP length: 101
effective length of query: 229
effective length of database: 11,313,751
effective search space: 2590848979
effective search space used: 2590848979
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 104 (44.7 bits)