TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000037_1.0 # Protein # SECIS binding protein 2 (SBP2) #
Homo sapiens # Complete
         (854 letters)

Database: P.polycephalum/genome.fa 
           297,657 sequences; 319,018,052 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Contig12166  2663 7351                                                 47   4e-04
Contig6889  8034 12786                                                 40   0.050
Contig43364  572 572                                                   38   0.19 
Contig32948  1235 1235                                                 35   1.2  
Contig17095  4082 8484                                                 35   1.6  
Contig11861  3025 3469                                                 34   3.6  
Contig11896  2613 6597                                                 33   4.7  
Contig5755  7079 13405                                                 33   6.1  
Contig2512  11414 11444                                                33   6.1  
Contig833  22439 46269                                                 33   6.1  
Contig28931  1004 1004                                                 33   8.0  
Contig14659  1446 1446                                                 33   8.0  
Contig12309  2472 3172                                                 33   8.0  

>Contig12166  2663 7351
          Length = 7351

 Score = 47.0 bits (110), Expect = 4e-04
 Identities = 23/56 (41%), Positives = 34/56 (60%)
 Frame = +1

Query: 506  NPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERKQRLQENAVSPAFTSDDT 561
            +P+ ++     +GK+REIPK KK +  KKIILKERQE+K+    +  +    S  T
Sbjct: 3232 HPVFAAVSTQTRGKEREIPKKKKVSVTKKIILKERQEKKENEHNSETNDLIPSPST 3399


>Contig6889  8034 12786
          Length = 12786

 Score = 40.0 bits (92), Expect = 0.050
 Identities = 22/60 (36%), Positives = 35/60 (58%)
 Frame = +2

Query: 490   ASGERGRRMSQMKTPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERKQRLQE 549
             A GERGR+  + KT    L +SAP   K K+R+  K KK    K+   K ++++K++ +E
Sbjct: 11828 AWGERGRKKCRKKTVRRALKTSAPWCVKEKKRKKRKEKKQKETKRKEKKRKEKKKRKEKE 12007


>Contig43364  572 572
          Length = 572

 Score = 38.1 bits (87), Expect = 0.19
 Identities = 17/44 (38%), Positives = 28/44 (63%)
 Frame = -3

Query: 692 VIISPNCEKIQSKGGLDDTLHTIIDYACEQNIPFVFALNRKALG 735
           +I   + E+I S+GGL+D ++ +I    EQ +   FAL+RK +G
Sbjct: 570 MIYKKDIEEITSEGGLNDQIYEVIKACQEQEVTLAFALSRKKIG 439


>Contig32948  1235 1235
          Length = 1235

 Score = 35.4 bits (80), Expect = 1.2
 Identities = 24/102 (23%), Positives = 45/102 (44%)
 Frame = -3

Query: 498  MSQMKTPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERKQRLQENAVSPAFT 557
            ++  +TPH P    A   +K +++E  K K+    KK   KE+++ K++ +E A      
Sbjct: 1113 LAMPRTPHTPRTPGAKEKEKEREKEKEKEKEKEKQKKQKEKEKEKEKEKEKEKA------ 952

Query: 558  SDDTQDGESGGDDQFPEQAELSGPEGMDELISTPSVEDKSEE 599
             +  +  +  G ++  E  + S  E  D        E + EE
Sbjct: 951  KEKEKAKKKSGKEKNKEDTDSSNAEREDSETGDKEGEKEGEE 826


>Contig17095  4082 8484
          Length = 8484

 Score = 35.0 bits (79), Expect = 1.6
 Identities = 24/98 (24%), Positives = 43/98 (43%)
 Frame = -1

Query: 502 KTPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERKQRLQENAVSPAFTSDDT 561
           +TPH P    A   +K +++E  K K+    KK   KE+++ K++ +E A       +  
Sbjct: 894 RTPHTPRTPGAKEKEKEREKEKEKEKEKEKQKKQKEKEKEKEKEKEKEKA------KEKE 733

Query: 562 QDGESGGDDQFPEQAELSGPEGMDELISTPSVEDKSEE 599
           +  +  G ++  E  + S  E  D        E + EE
Sbjct: 732 KAKKKSGKEKNKEDTDSSNAEREDSETGDKEGEKEGEE 619


>Contig11861  3025 3469
          Length = 3469

 Score = 33.9 bits (76), Expect = 3.6
 Identities = 27/93 (29%), Positives = 41/93 (44%)
 Frame = +1

Query: 1    MASEGPREPESEGIKLSADVKPFVPRFAGLNVAWLESSEACVFPSSAATYYPFVQEPPVT 60
            ++S  P  P S  + LS    PF+  F   +   L SS     PSS  +  PF+  P   
Sbjct: 1837 LSSFPPFIPFSPFLSLSLPFSPFILPFLPPS---LPSSHPSSLPSSPPSSLPFLLPP--- 1998

Query: 61   EQKIYTEDMAFGASTFPPQYLSSEITLHPYAYS 93
                 +  + F   +F P +LSS ++  PY +S
Sbjct: 1999 ---FLSSSIPFFLPSFLPPFLSSALSFIPYPFS 2088


>Contig11896  2613 6597
          Length = 6597

 Score = 33.5 bits (75), Expect = 4.7
 Identities = 40/170 (23%), Positives = 63/170 (37%), Gaps = 16/170 (9%)
 Frame = +2

Query: 202  SRIIAKNVSTSKPEFEFTTLDFPELQGAENNMSEIQKQ--------------PKWGPVHS 247
            S +  +N ST +  +E  T    +     NN  +I K               P      S
Sbjct: 3023 SNLATRNTSTIQTLYENKT----KQTNINNNKKKINKNGIDIKFNTKVLEIFPAGQVTTS 3190

Query: 248  VSTDISLLREVVKPAAVLSKGEIVVKNNPNESVTANAATNS-PSCTRELSWTP-MGYVVR 305
              T I  +R   +      + E+   +NPN    ANA +NS PS +   S +P     + 
Sbjct: 3191 ELTQIYSVRNQARKKKEQKQKELEKASNPNPESAANANSNSNPSPSPSPSLSPSSSSALP 3370

Query: 306  QTLSTELSAAPKNVTSMINLKTIASSADPKNVSIPSSEALSSDPSYNKEK 355
             + ST  S  P +  S +     +SS+     + P     ++ P   KEK
Sbjct: 3371 SSSSTPASPNPSSAASQLMPPPSSSSSSSAPTTSPFFNVATTSPKIKKEK 3520


>Contig5755  7079 13405
          Length = 13405

 Score = 33.1 bits (74), Expect = 6.1
 Identities = 25/87 (28%), Positives = 41/87 (47%)
 Frame = +1

Query: 540  RQERKQRLQENAVSPAFTSDDTQDGESGGDDQFPEQAELSGPEGMDELISTPSVEDKSEE 599
            +Q+  +   + A SP+ +S+D ++ E  GD +  E  E +G E   +       E+K EE
Sbjct: 5593 KQQPNKPATQKAPSPS-SSEDKEEDEELGDHR--EHREKTGAEPEHKKEEEEEEEEKEEE 5763

Query: 600  PPGTELQRDTEASHLAPNHTTFPKIHS 626
                +L   +  SHL P+      IHS
Sbjct: 5764 EDHHDLPPPSTPSHLLPHPRHLLVIHS 5844


>Contig2512  11414 11444
          Length = 11444

 Score = 33.1 bits (74), Expect = 6.1
 Identities = 54/194 (27%), Positives = 73/194 (37%), Gaps = 31/194 (15%)
 Frame = +3

Query: 92    YSPYTLDSTQNVYSVPGSQYLYNQ---PSCYRGFQTVKH-----RNENTCPLPQEMKALF 143
             YS +T+ S     + P   +  N    P     F T+ H      N++T   PQ    + 
Sbjct: 9987  YSTHTISSINISTTTPNLGHTLNSQFTPLSLVKFTTIPHSQGAKNNKSTASTPQLTSHVL 10166

Query: 144   XXXXXXXXXXXXQQKFDSERADGTISSEIKSARGSHHLSIYAENSLK--------SDGYH 195
                         Q   D  R  G+ S   K    +HHL+  +E+  K        S   +
Sbjct: 10167 TLDH--------QWSNDRNRGSGS-SGFSKGGTVTHHLNSKSEDKEKN*YQYNPSSFSLY 10319

Query: 196   KRTDRKSRIIAKNVSTSKPEFEFTTLDFPELQGAENNMSEI-QKQP-----KWGP----V 245
                  KS  I +      P  +FTT+  P  QGAENN   I Q+QP     K GP    +
Sbjct: 10320 HHPQSKSHSIQR-----LPLMKFTTIPHP--QGAENNKGRITQRQPPNPHIKGGPNTSLL 10478

Query: 246   HS-----VSTDISL 254
             HS      ST ISL
Sbjct: 10479 HSGSNALTSTSISL 10520


>Contig833  22439 46269
          Length = 46269

 Score = 33.1 bits (74), Expect = 6.1
 Identities = 24/77 (31%), Positives = 35/77 (45%), Gaps = 2/77 (2%)
 Frame = +2

Query: 165   DGTISSEIKSARGSHHLSIYAE--NSLKSDGYHKRTDRKSRIIAKNVSTSKPEFEFTTLD 222
             DG + S     R S+H  ++    +S K     K+  +K +   K   TSKP  E  T  
Sbjct: 23057 DGYLLSNHGYHRRSYHQLLWQTGVSSQKKKKKKKKKKKKKKKKKKKNQTSKPCNEIPTQT 23236

Query: 223   FPELQGAENNMSEIQKQ 239
             F ELQ A N +  +Q +
Sbjct: 23237 FSELQNAYNEIGGVQNE 23287


>Contig28931  1004 1004
          Length = 1004

 Score = 32.7 bits (73), Expect = 8.0
 Identities = 17/58 (29%), Positives = 30/58 (51%)
 Frame = -1

Query: 489 CASGERGRRMSQMKTPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERKQR 546
           C  GE  R   +   PH+    +   +K+ K+R+  + KK    KK   K+++ERK++
Sbjct: 809 CLGGEEKRERRKNCAPHS*NQCTTVCLKRKKERKKKERKKKRRKKKERRKKKEERKKK 636


>Contig14659  1446 1446
          Length = 1446

 Score = 32.7 bits (73), Expect = 8.0
 Identities = 28/100 (28%), Positives = 49/100 (49%), Gaps = 11/100 (11%)
 Frame = +1

Query: 522 EIPKAKKPTSLKKIILKERQERKQRLQENAVSPAFT-------SDDTQDGESGGDDQFPE 574
           ++PK  KP  +K+   KE +E+K++ +E   S   +       SDD+ D ES   D+  E
Sbjct: 379 DMPKHVKPDEVKR--RKEEEEKKKKQEEKDESEEESDDSSDDSSDDSSDNES--SDESSE 546

Query: 575 QAELSGPEGMDEL-ISTPSVEDKSEEP---PGTELQRDTE 610
            ++ S  +  DE     P  ++   +P    GT+ +R+ E
Sbjct: 547 SSDSSSDDSSDESEEEAPKKKEDKAKPVVAQGTKNKRNVE 666


>Contig12309  2472 3172
          Length = 3172

 Score = 32.7 bits (73), Expect = 8.0
 Identities = 24/74 (32%), Positives = 34/74 (45%)
 Frame = -1

Query: 537  LKERQERKQRLQENAVSPAFTSDDTQDGESGGDDQFPEQAELSGPEGMDELISTPSVEDK 596
            LKE +++K   +E A S +  S   +   S GD++  E+ E  G E  DE       ED 
Sbjct: 3061 LKETRKKKTAEREAASSESDDSPSARKTRSRGDNKEDEEGEEEGEEEEDE--DEEGGEDG 2888

Query: 597  SEEPPGTELQRDTE 610
             EE    E + D E
Sbjct: 2887 EEEEESEEGEEDEE 2846


  Database: P.polycephalum/genome.fa
    Posted date:  Nov 21, 2011  7:46 PM
  Number of letters in database: 319,018,052
  Number of sequences in database:  297,657
  
Lambda     K      H
   0.311    0.128    0.365 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 279,024,581
Number of Sequences: 297657
Number of extensions: 4011135
Number of successful extensions: 32885
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 23941
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 31800
length of query: 854
length of database: 106,339,350
effective HSP length: 122
effective length of query: 732
effective length of database: 70,025,196
effective search space: 51258443472
effective search space used: 51258443472
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 72 (32.3 bits)