BLASTP 2.2.23+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Stephen F. Altschul, John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. RID: UMHJRN0Y016 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 10,635,453 sequences; 3,627,481,469 total letters Query= SelM.Thalassioira_pseudonana. gi|209487133|gb|AAFD02000028.1|:subseq(10000,12000).pep Length=79 Score E Sequences producing significant alignments: (Bits) Value ref|NP_536355.1| selenoprotein M precursor [Homo sapiens] >sp... 41.6 6e-04 ALIGNMENTS >ref|NP_536355.1| selenoprotein M precursor [Homo sapiens] sp|Q8WWX9.3|SELM_HUMAN RecName: Full=Selenoprotein M; Short=SelM; Flags: Precursor gb|AAK95397.1| selenoprotein SelM [Homo sapiens] gb|AAH30236.1| Selenoprotein M [Homo sapiens] gb|AAH13421.1| Selenoprotein M [Homo sapiens] gb|AAH68004.1| Selenoprotein M [Homo sapiens] Length=145 Score = 41.6 bits (96), Expect = 6e-04, Method: Compositional matrix adjust. Identities = 27/79 (34%), Positives = 44/79 (55%), Gaps = 6/79 (7%) Query 3 IESCSGXALNRLPELKSFLKDLNGVDLYKNVEVHFISGKRAVLTIFEGGR--ELEKITLS 60 +E+C G LNRL E+K+F+ + Y N+ + + G L + G R ELE+I LS Sbjct 42 VETCGGUQLNRLKEVKAFVT--QDIPFYHNLVMKHLPGADPELVLL-GRRYEELERIPLS 98 Query 61 DYNDKEKLHTLFAEKGFVK 79 + +E+++ L E GF + Sbjct 99 EMT-REEINALVQELGFYR 116 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects Posted date: Mar 22, 2010 5:43 PM Number of letters in database: 82,119,894 Number of sequences in database: 230,407 Lambda K H 0.318 0.140 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 230407 Number of Hits to DB: 1820418 Number of extensions: 59437 Number of successful extensions: 104 Number of sequences better than 100: 2 Number of HSP's better than 100 without gapping: 0 Number of HSP's gapped: 104 Number of HSP's successfully gapped: 2 Length of query: 79 Length of database: 82119894 Length adjustment: 50 Effective length of query: 29 Effective length of database: 70599544 Effective search space: 2047386776 Effective search space used: 2047386776 T: 11 A: 40 X1: 16 (7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (20.4 bits) S2: 52 (24.6 bits)