BLASTP 2.2.23+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Stephen F. Altschul, John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. RID: UMHSJ35S012 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 10,635,453 sequences; 3,627,481,469 total letters Query= SelM.Aurecoccus_anophagefferens. Regio1 scaffold_12. [translate(1)] Length=90 Score E Sequences producing significant alignments: (Bits) Value ref|NP_444497.1| selenoprotein M precursor [Mus musculus] >sp... 64.3 5e-11 pdb|2A2P|A Chain A, Solution Structure Of Selm From Mus Musculus 64.3 7e-11 dbj|BAE43327.1| unnamed protein product [Mus musculus] 62.4 2e-10 gb|AAH19792.2| Selenoprotein [Mus musculus] 50.1 1e-06 ref|NP_444332.1| 15 kDa selenoprotein precursor [Mus musculus... 48.5 3e-06 sp|Q9ERR7.3|SEP15_MOUSE RecName: Full=15 kDa selenoprotein; F... 48.5 3e-06 gb|AAH19742.1| Selm protein [Mus musculus] 36.2 0.017 ref|NP_620095.1| solute carrier family 25, member 36 [Mus mus... 28.9 3.0 ref|XP_999727.1| PREDICTED: hypothetical protein [Mus musculus] 28.5 3.5 dbj|BAC28704.1| unnamed protein product [Mus musculus] 28.5 3.5 ref|XP_997087.1| PREDICTED: hypothetical protein [Mus musculus] 28.5 3.7 gb|AAH04098.1| Slc25a36 protein [Mus musculus] 27.7 5.9 gb|EDL25548.1| mCG1534 [Mus musculus] 27.3 8.9 ref|NP_001030035.1| transmembrane protein 136 [Mus musculus] ... 27.3 9.3 gb|AAI39047.1| Transmembrane protein 136 [Mus musculus] 26.9 9.4 ALIGNMENTS >ref|NP_444497.1| selenoprotein M precursor [Mus musculus] sp|Q8VHC3.3|SELM_MOUSE RecName: Full=Selenoprotein M; Short=SelM; Flags: Precursor gb|AAK95398.1| selenoprotein SelM [Mus musculus] dbj|BAE43252.1| unnamed protein product [Mus musculus] dbj|BAC29028.2| unnamed protein product [Mus musculus] gb|AAI47333.1| Selenoprotein M [Mus musculus] gb|AAI47334.1| Selenoprotein M [Mus musculus] Length=145 Score = 64.3 bits (155), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 35/88 (39%), Positives = 53/88 (60%), Gaps = 3/88 (3%) Query 1 LAAAATGKIETCSAXKLNKLPEVKRFVKESGHADTYEGLEIDYVRGKPPTLIMMDGD-AE 59 L A G++ETC +LN+L EVK FV E Y L + ++ G P L+++ + E Sbjct 34 LRGLARGRVETCGGUQLNRLKEVKAFVTED--IQLYHNLVMKHLPGADPELVLLSRNYQE 91 Query 60 VERVDLAPYSTDELHALMQEKGFARKEA 87 +ER+ L+ + DE++AL+QE GF RK A Sbjct 92 LERIPLSQMTRDEINALVQELGFYRKSA 119 >pdb|2A2P|A Chain A, Solution Structure Of Selm From Mus Musculus Length=129 Score = 64.3 bits (155), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 35/88 (39%), Positives = 53/88 (60%), Gaps = 3/88 (3%) Query 1 LAAAATGKIETCSAXKLNKLPEVKRFVKESGHADTYEGLEIDYVRGKPPTLIMMDGD-AE 59 L A G++ETC +LN+L EVK FV E Y L + ++ G P L+++ + E Sbjct 11 LRGLARGRVETCGGCQLNRLKEVKAFVTED--IQLYHNLVMKHLPGADPELVLLSRNYQE 68 Query 60 VERVDLAPYSTDELHALMQEKGFARKEA 87 +ER+ L+ + DE++AL+QE GF RK A Sbjct 69 LERIPLSQMTRDEINALVQELGFYRKSA 96 >dbj|BAE43327.1| unnamed protein product [Mus musculus] Length=145 Score = 62.4 bits (150), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 34/88 (38%), Positives = 52/88 (59%), Gaps = 3/88 (3%) Query 1 LAAAATGKIETCSAXKLNKLPEVKRFVKESGHADTYEGLEIDYVRGKPPTLIMMDGD-AE 59 L A G++ETC +LN+L EVK FV E Y L + ++ G P L+++ + E Sbjct 34 LRGLARGRVETCGGUQLNRLKEVKAFVTED--IQLYHNLVMKHLPGADPELVLLSRNYQE 91 Query 60 VERVDLAPYSTDELHALMQEKGFARKEA 87 +ER+ L+ + DE++ L+QE GF RK A Sbjct 92 LERIPLSQMTRDEINELVQELGFYRKSA 119 >gb|AAH19792.2| Selenoprotein [Mus musculus] Length=162 Score = 50.1 bits (118), Expect = 1e-06, Method: Compositional matrix adjust. Identities = 25/73 (34%), Positives = 42/73 (57%), Gaps = 3/73 (4%) Query 9 IETCSAXKLNKLPEVKRFVKESGHADTYEGLEIDYVRGKPPTLIMMDGDAEV-ERVDLAP 67 +E C KL +LP+V+ FV+ S + GL+I YVRG P L ++D + + E + + Sbjct 88 LEVCGU-KLGRLPQVQAFVR-SDKPKLFRGLQIKYVRGSDPVLKLLDDNGNIAEELSILK 145 Query 68 YSTDELHALMQEK 80 ++TD + + EK Sbjct 146 WNTDSVEEFLSEK 158 >ref|NP_444332.1| 15 kDa selenoprotein precursor [Mus musculus] gb|AAG31765.1|AF288740_1 15 kDa selenoprotein [Mus musculus] dbj|BAC55255.1| unnamed protein product [Mus musculus] dbj|BAE35012.1| unnamed protein product [Mus musculus] Length=162 Score = 48.5 bits (114), Expect = 3e-06, Method: Compositional matrix adjust. Identities = 24/73 (32%), Positives = 41/73 (56%), Gaps = 3/73 (4%) Query 9 IETCSAXKLNKLPEVKRFVKESGHADTYEGLEIDYVRGKPPTLIMMDGDAEV-ERVDLAP 67 +E C KL + P+V+ FV+ S + GL+I YVRG P L ++D + + E + + Sbjct 88 LEVCGU-KLGRFPQVQAFVR-SDKPKLFRGLQIKYVRGSDPVLKLLDDNGNIAEELSILK 145 Query 68 YSTDELHALMQEK 80 ++TD + + EK Sbjct 146 WNTDSVEEFLSEK 158 >sp|Q9ERR7.3|SEP15_MOUSE RecName: Full=15 kDa selenoprotein; Flags: Precursor dbj|BAE34803.1| unnamed protein product [Mus musculus] Length=162 Score = 48.5 bits (114), Expect = 3e-06, Method: Compositional matrix adjust. Identities = 24/73 (32%), Positives = 41/73 (56%), Gaps = 3/73 (4%) Query 9 IETCSAXKLNKLPEVKRFVKESGHADTYEGLEIDYVRGKPPTLIMMDGDAEV-ERVDLAP 67 +E C KL + P+V+ FV+ S + GL+I YVRG P L ++D + + E + + Sbjct 88 LEVCGU-KLGRFPQVQAFVR-SDKPKLFRGLQIKYVRGSDPVLKLLDDNGNIAEELSILK 145 Query 68 YSTDELHALMQEK 80 ++TD + + EK Sbjct 146 WNTDSVEEFLSEK 158 >gb|AAH19742.1| Selm protein [Mus musculus] Length=74 Score = 36.2 bits (82), Expect = 0.017, Method: Compositional matrix adjust. Identities = 18/48 (37%), Positives = 32/48 (66%), Gaps = 1/48 (2%) Query 41 IDYVRGKPPTLIMMDGD-AEVERVDLAPYSTDELHALMQEKGFARKEA 87 + ++ G P L+++ + E+ER+ L+ + DE++AL+QE GF RK A Sbjct 1 MKHLPGADPELVLLSRNYQELERIPLSQMTRDEINALVQELGFYRKSA 48 >ref|NP_620095.1| solute carrier family 25, member 36 [Mus musculus] sp|Q922G0.1|S2536_MOUSE RecName: Full=Solute carrier family 25 member 36 gb|AAH08171.1| Solute carrier family 25, member 36 [Mus musculus] dbj|BAC35461.1| unnamed protein product [Mus musculus] dbj|BAC37391.1| unnamed protein product [Mus musculus] dbj|BAE20619.1| unnamed protein product [Mus musculus] gb|EDL20973.1| solute carrier family 25, member 36, isoform CRA_b [Mus musculus] Length=311 Score = 28.9 bits (63), Expect = 3.0, Method: Compositional matrix adjust. Identities = 13/29 (44%), Positives = 15/29 (51%), Gaps = 0/29 (0%) Query 26 FVKESGHADTYEGLEIDYVRGKPPTLIMM 54 V+E G+ Y GL VR P T IMM Sbjct 271 IVQEEGYGSLYRGLTTHLVRQIPNTAIMM 299 >ref|XP_999727.1| PREDICTED: hypothetical protein [Mus musculus] Length=1209 Score = 28.5 bits (62), Expect = 3.5, Method: Composition-based stats. Identities = 17/63 (26%), Positives = 28/63 (44%), Gaps = 0/63 (0%) Query 7 GKIETCSAXKLNKLPEVKRFVKESGHADTYEGLEIDYVRGKPPTLIMMDGDAEVERVDLA 66 GK++T ++ K+ KLP KRF + + ID PP+ EV+ L Sbjct 609 GKLQTNTSPKMPKLPPWKRFTHVDSRQPCWRVIVIDPEESIPPSTAKQSNIVEVKEEPLP 668 Query 67 PYS 69 ++ Sbjct 669 AWT 671 >dbj|BAC28704.1| unnamed protein product [Mus musculus] Length=172 Score = 28.5 bits (62), Expect = 3.5, Method: Compositional matrix adjust. Identities = 13/28 (46%), Positives = 15/28 (53%), Gaps = 0/28 (0%) Query 27 VKESGHADTYEGLEIDYVRGKPPTLIMM 54 V+E G+ Y GL VR P T IMM Sbjct 133 VQEEGYGSLYRGLTTHLVRQIPNTAIMM 160 >ref|XP_997087.1| PREDICTED: hypothetical protein [Mus musculus] Length=1185 Score = 28.5 bits (62), Expect = 3.7, Method: Composition-based stats. Identities = 17/63 (26%), Positives = 28/63 (44%), Gaps = 0/63 (0%) Query 7 GKIETCSAXKLNKLPEVKRFVKESGHADTYEGLEIDYVRGKPPTLIMMDGDAEVERVDLA 66 GK++T ++ K+ KLP KRF + + ID PP+ EV+ L Sbjct 609 GKLQTNTSPKMPKLPPWKRFTHVDSRQPCWRVIVIDPEESIPPSTAKQSNIVEVKEEPLP 668 Query 67 PYS 69 ++ Sbjct 669 AWT 671 >gb|AAH04098.1| Slc25a36 protein [Mus musculus] Length=72 Score = 27.7 bits (60), Expect = 5.9, Method: Compositional matrix adjust. Identities = 13/28 (46%), Positives = 15/28 (53%), Gaps = 0/28 (0%) Query 27 VKESGHADTYEGLEIDYVRGKPPTLIMM 54 V+E G+ Y GL VR P T IMM Sbjct 33 VQEEGYGSLYRGLTTHLVRQIPNTAIMM 60 >gb|EDL25548.1| mCG1534 [Mus musculus] Length=270 Score = 27.3 bits (59), Expect = 8.9, Method: Compositional matrix adjust. Identities = 8/27 (29%), Positives = 19/27 (70%), Gaps = 0/27 (0%) Query 18 NKLPEVKRFVKESGHADTYEGLEIDYV 44 N L +++ F++E+GH ++ G +D++ Sbjct 162 NPLLQMRWFLRETGHYHSFTGDVVDFL 188 >ref|NP_001030035.1| transmembrane protein 136 [Mus musculus] sp|Q3TYE7.1|TM136_MOUSE RecName: Full=Transmembrane protein 136 dbj|BAE34616.1| unnamed protein product [Mus musculus] gb|AAI39048.1| Transmembrane protein 136 [Mus musculus] Length=245 Score = 27.3 bits (59), Expect = 9.3, Method: Compositional matrix adjust. Identities = 8/27 (29%), Positives = 19/27 (70%), Gaps = 0/27 (0%) Query 18 NKLPEVKRFVKESGHADTYEGLEIDYV 44 N L +++ F++E+GH ++ G +D++ Sbjct 137 NPLLQMRWFLRETGHYHSFTGDVVDFL 163 >gb|AAI39047.1| Transmembrane protein 136 [Mus musculus] Length=245 Score = 26.9 bits (58), Expect = 9.4, Method: Compositional matrix adjust. Identities = 8/27 (29%), Positives = 19/27 (70%), Gaps = 0/27 (0%) Query 18 NKLPEVKRFVKESGHADTYEGLEIDYV 44 N L +++ F++E+GH ++ G +D++ Sbjct 137 NPLLQMRWFLRETGHYHSFTGDVVDFL 163 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects Posted date: Mar 22, 2010 5:43 PM Number of letters in database: 56,585,266 Number of sequences in database: 144,787 Lambda K H 0.312 0.131 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 144787 Number of Hits to DB: 1314595 Number of extensions: 47118 Number of successful extensions: 53 Number of sequences better than 100: 13 Number of HSP's better than 100 without gapping: 0 Number of HSP's gapped: 53 Number of HSP's successfully gapped: 13 Length of query: 90 Length of database: 56585266 Length adjustment: 61 Effective length of query: 29 Effective length of database: 47753259 Effective search space: 1384844511 Effective search space used: 1384844511 T: 11 A: 40 X1: 16 (7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (20.8 bits) S2: 50 (23.9 bits)