BLASTP 2.2.23+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Stephen F. Altschul, John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. RID: UMK14CPV01N Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects 10,635,453 sequences; 3,627,481,469 total letters Query= scaffold_323 [translate(1)] Length=147 Score E Sequences producing significant alignments: (Bits) Value ref|XP_647424.2| 15 kDa selenoprotein [Dictyostelium discoide... 179 3e-46 ref|XP_629848.1| hypothetical protein DDB_G0292128 [Dictyoste... 27.7 1.7 ref|XP_645111.1| NADP-dependent malate dehydrogenase [Dictyos... 27.3 2.4 ref|XP_639211.1| hypothetical protein DDB_G0283189 [Dictyoste... 27.3 2.5 ref|XP_645603.2| IPT/TIG domain-containing protein [Dictyoste... 26.2 5.2 ref|XP_645705.1| hypothetical protein DDB_G0271398 [Dictyoste... 25.8 6.0 ref|XP_639009.2| patatin family protein [Dictyostelium discoi... 25.8 6.9 ref|XP_642245.1| hypothetical protein DDB_G0278259 [Dictyoste... 25.4 9.0 ALIGNMENTS >ref|XP_647424.2| 15 kDa selenoprotein [Dictyostelium discoideum AX4] gb|EAL73651.2| 15 kDa selenoprotein [Dictyostelium discoideum AX4] Length=166 Score = 179 bits (454), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 86/148 (58%), Positives = 111/148 (75%), Gaps = 1/148 (0%) Query 1 LYLCVVVLLFIFIGESSSISIT-SPKHSCHELGFTDSLLCSTCKDFAEFVGDSDLNNECA 59 ++L + L I I + +S + SCHELGFTD+LLCSTCKDFAEFVGDS +EC+ Sbjct 17 IFLLAAIFLCILINDCQGVSSPGTSASSCHELGFTDALLCSTCKDFAEFVGDSAFGHECS 76 Query 60 KCCAEESENIKKTYLSATLIACKXRLGAFPHIKDFIDNKAKDYNKFKFTHEQGANPRLVL 119 CC+EESE +KKTY S +L+ C+ RLGA P IK+FI+NKAK Y+K +E GANPRL L Sbjct 77 ICCSEESEKVKKTYQSVSLVVCQURLGAHPQIKEFINNKAKLYSKLSVKYENGANPRLTL 136 Query 120 RDTEGKEEEISIDGWKSENLEEFLIQNN 147 D+EGK+E+I+I+ WK ENLEE+L+QNN Sbjct 137 VDSEGKKEDINIESWKLENLEEYLVQNN 164 >ref|XP_629848.1| hypothetical protein DDB_G0292128 [Dictyostelium discoideum AX4] gb|EAL61420.1| hypothetical protein DDB_G0292128 [Dictyostelium discoideum AX4] Length=1569 Score = 27.7 bits (60), Expect = 1.7, Method: Compositional matrix adjust. Identities = 12/25 (48%), Positives = 16/25 (64%), Gaps = 0/25 (0%) Query 90 HIKDFIDNKAKDYNKFKFTHEQGAN 114 +I+DFI N DY K++ HE G N Sbjct 554 NIRDFIFNHIIDYYKYESVHEVGGN 578 >ref|XP_645111.1| NADP-dependent malate dehydrogenase [Dictyostelium discoideum AX4] sp|Q6TU48.1|MAOX_DICDI RecName: Full=NADP-dependent malic enzyme; Short=NADP-ME; AltName: Full=SrfA-induced gene A protein gb|AAQ95658.1| malic enzyme [Dictyostelium discoideum] gb|EAL71186.1| NADP-dependent malate dehydrogenase [Dictyostelium discoideum AX4] Length=544 Score = 27.3 bits (59), Expect = 2.4, Method: Composition-based stats. Identities = 14/46 (30%), Positives = 24/46 (52%), Gaps = 6/46 (13%) Query 9 LFIFIGESSSISITSPKHSCHELGFTDSLLCSTCKDFAEFVGDSDL 54 ++IF G + S+ KH TD+++ + K A FV DS++ Sbjct 446 MYIFPGLGLAASVCEAKH------VTDAMIITAAKTLASFVEDSEV 485 >ref|XP_639211.1| hypothetical protein DDB_G0283189 [Dictyostelium discoideum AX4] sp|Q54RD4.1|DPOE1_DICDI RecName: Full=DNA polymerase epsilon catalytic subunit A; AltName: Full=DNA polymerase II subunit A gb|EAL65833.1| hypothetical protein DDB_G0283189 [Dictyostelium discoideum AX4] Length=2380 Score = 27.3 bits (59), Expect = 2.5, Method: Compositional matrix adjust. Identities = 18/53 (33%), Positives = 24/53 (45%), Gaps = 5/53 (9%) Query 9 LFIFIGESSSISITSPKHSCHELGFTDSLLCSTCKDFAEFVGDSDLNNECAKC 61 L I ESS++S E G S ST K AEF+GD + ++ C Sbjct 1022 LIELITESSNMS-----RKLEEYGTQKSSAISTAKKLAEFLGDDMIKDKGLSC 1069 >ref|XP_645603.2| IPT/TIG domain-containing protein [Dictyostelium discoideum AX4] gb|EAL71642.2| IPT/TIG domain-containing protein [Dictyostelium discoideum AX4] Length=885 Score = 26.2 bits (56), Expect = 5.2, Method: Compositional matrix adjust. Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 0/34 (0%) Query 91 IKDFIDNKAKDYNKFKFTHEQGANPRLVLRDTEG 124 +K + DN+ +N F T+E G+ P L D EG Sbjct 152 LKFYYDNRLGKWNFFDATYEMGSIPVDPLLDGEG 185 >ref|XP_645705.1| hypothetical protein DDB_G0271398 [Dictyostelium discoideum AX4] gb|EAL71829.1| hypothetical protein DDB_G0271398 [Dictyostelium discoideum AX4] Length=1874 Score = 25.8 bits (55), Expect = 6.0, Method: Compositional matrix adjust. Identities = 9/25 (36%), Positives = 16/25 (64%), Gaps = 0/25 (0%) Query 105 FKFTHEQGANPRLVLRDTEGKEEEI 129 K+ EQ A P ++L++ + K +EI Sbjct 41 IKYLQEQSATPEILLQNLKSKSDEI 65 >ref|XP_639009.2| patatin family protein [Dictyostelium discoideum AX4] gb|EAL65654.2| patatin family protein [Dictyostelium discoideum AX4] Length=1514 Score = 25.8 bits (55), Expect = 6.9, Method: Compositional matrix adjust. Identities = 21/69 (30%), Positives = 30/69 (43%), Gaps = 4/69 (5%) Query 8 LLFIFIGESSSISITSPKHSC----HELGFTDSLLCSTCKDFAEFVGDSDLNNECAKCCA 63 L+ FIG S S T+ H H + L ST D F+G++ +N+ K Sbjct 128 LVVSFIGTSGSGKSTTIGHLTKNYNHPIPGNHETLSSTSSDINSFIGETMGSNKNYKYLI 187 Query 64 EESENIKKT 72 +SE I T Sbjct 188 IDSEGINGT 196 >ref|XP_642245.1| hypothetical protein DDB_G0278259 [Dictyostelium discoideum AX4] sp|Q54YF7.1|MANB_DICDI RecName: Full=Alpha-mannosidase B; Flags: Precursor gb|EAL68302.1| hypothetical protein DDB_G0278259 [Dictyostelium discoideum AX4] Length=1035 Score = 25.4 bits (54), Expect = 9.0, Method: Composition-based stats. Identities = 12/44 (27%), Positives = 19/44 (43%), Gaps = 0/44 (0%) Query 94 FIDNKAKDYNKFKFTHEQGANPRLVLRDTEGKEEEISIDGWKSE 137 I N +N F+ ++ N RL+ D + + I GW E Sbjct 716 LIYNDTTSFNPFETDNDNNNNSRLIKNDQYFEIDNIVASGWDKE 759 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding environmental samples from WGS projects Posted date: Mar 22, 2010 5:43 PM Number of letters in database: 7,458,971 Number of sequences in database: 13,913 Lambda K H 0.323 0.138 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 13913 Number of Hits to DB: 483 Number of extensions: 25 Number of successful extensions: 0 Number of sequences better than 100: 0 Number of HSP's better than 100 without gapping: 0 Number of HSP's gapped: 0 Number of HSP's successfully gapped: 0 Length of query: 147 Length of database: 7458971 Length adjustment: 87 Effective length of query: 60 Effective length of database: 6248540 Effective search space: 374912400 Effective search space used: 374912400 T: 11 A: 40 X1: 16 (7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (20.4 bits) S2: 45 (21.9 bits)