TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= scaffold_27 dpurpureum selr protein
         (132 letters)

Database: genome.fa 
           3590 sequences; 66,210,174 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAQM02000063.1| Toxoplasma gondii GT1 gcontig_1107000978084, ...    44   6e-05
gb|AAQM02000007.1| Toxoplasma gondii GT1 gcontig_1107000855690, ...    29   1.5  
gb|AAQM02000039.1| Toxoplasma gondii GT1 gcontig_1107000860484, ...    29   1.9  
gb|AAQM02000006.1| Toxoplasma gondii GT1 gcontig_1107000875718, ...    28   2.5  
gb|AAQM02000637.1| Toxoplasma gondii GT1 gcontig_1107000829554, ...    28   3.3  
gb|AAQM02000123.1| Toxoplasma gondii GT1 gcontig_1107000946696, ...    28   3.3  
gb|AAQM02000035.1| Toxoplasma gondii GT1 gcontig_1107000925766, ...    28   3.3  
gb|AAQM02000022.1| Toxoplasma gondii GT1 gcontig_1107000925796, ...    28   3.3  
gb|AAQM02000183.1| Toxoplasma gondii GT1 gcontig_1107000996866, ...    28   4.3  
gb|AAQM02000277.1| Toxoplasma gondii GT1 gcontig_1107000974560, ...    27   5.6  
gb|AAQM02000027.1| Toxoplasma gondii GT1 gcontig_1107000955098, ...    27   5.6  
gb|AAQM02000070.1| Toxoplasma gondii GT1 gcontig_1107000852474, ...    27   9.5  
gb|AAQM02000054.1| Toxoplasma gondii GT1 gcontig_1107000855630, ...    27   9.5  
gb|AAQM02000050.1| Toxoplasma gondii GT1 gcontig_1107000845212, ...    27   9.5  
gb|AAQM02000041.1| Toxoplasma gondii GT1 gcontig_1107000869098, ...    27   9.5  
gb|AAQM02000018.1| Toxoplasma gondii GT1 gcontig_1107000879894, ...    27   9.5  
gb|AAQM02000009.1| Toxoplasma gondii GT1 gcontig_1107000925794, ...    27   9.5  

>gb|AAQM02000063.1| Toxoplasma gondii GT1 gcontig_1107000978084, whole genome shotgun
              sequence
          Length = 319274

 Score = 43.9 bits (102), Expect = 6e-05
 Identities = 16/29 (55%), Positives = 21/29 (72%)
 Frame = -2

Query: 40     EGLYECAGCGTPLFKSTQKFHSGCGWPSF 68
              +G + CA C  PLF ++ KF SGCGWP+F
Sbjct: 116017 KGYFACAACRHPLFPASAKFASGCGWPAF 115931



 Score = 42.0 bits (97), Expect = 2e-04
 Identities = 16/24 (66%), Positives = 18/24 (75%)
 Frame = -1

Query: 86     GRPRTEICCKKCGGHLGHVFKGEG 109
              G  R EI C  CGGHLGH+F+GEG
Sbjct: 115217 GMKRVEIVCANCGGHLGHLFEGEG 115146



 Score = 33.1 bits (74), Expect = 0.10
 Identities = 21/45 (46%), Positives = 23/45 (51%), Gaps = 3/45 (6%)
 Frame = -3

Query: 16     EQFRVLREKGTEAPFVGKYTDNKDEGLYE---CAGCGTPLFKSTQ 57
              EQFRVLR+KGTE P  GK          E   CA C   L  +TQ
Sbjct: 116610 EQFRVLRKKGTETPGTGKCRRRGGRASLETRSCA-CSPALLSATQ 116479


>gb|AAQM02000007.1| Toxoplasma gondii GT1 gcontig_1107000855690, whole genome shotgun
              sequence
          Length = 702265

 Score = 29.3 bits (64), Expect = 1.5
 Identities = 21/72 (29%), Positives = 29/72 (40%)
 Frame = -3

Query: 22     REKGTEAPFVGKYTDNKDEGLYECAGCGTPLFKSTQKFHSGCGWPSFFDSLPDAITFHSD 81
              REK    P      D +    +E A   +P F +   F +        D L ++    +D
Sbjct: 390707 REKAGSPPHRDAVLDAEKPAAFESAAHASPPFANAPDF*AAG------DQLVESRVDRND 390546

Query: 82     PSIPGRPRTEIC 93
              PSIP RP   IC
Sbjct: 390545 PSIPRRPGGGIC 390510


>gb|AAQM02000039.1| Toxoplasma gondii GT1 gcontig_1107000860484, whole genome shotgun
              sequence
          Length = 399002

 Score = 28.9 bits (63), Expect = 1.9
 Identities = 16/46 (34%), Positives = 21/46 (45%)
 Frame = +3

Query: 32     GKYTDNKDEGLYECAGCGTPLFKSTQKFHSGCGWPSFFDSLPDAIT 77
              G  +DNK E       C  PLF+ST+      G PS    L  A++
Sbjct: 112812 GVSSDNKHESQRRSCLCTCPLFQSTRCLSKDSGTPSTLHQLLPAVS 112949


>gb|AAQM02000006.1| Toxoplasma gondii GT1 gcontig_1107000875718, whole genome shotgun
              sequence
          Length = 720370

 Score = 28.5 bits (62), Expect = 2.5
 Identities = 19/61 (31%), Positives = 27/61 (44%), Gaps = 6/61 (9%)
 Frame = +1

Query: 53     FKSTQKFHSGCGWPSFFDS------LPDAITFHSDPSIPGRPRTEICCKKCGGHLGHVFK 106
              + ST +  SG  W + F        L  A  F  +PS  G  R+ + C+K  G  G +F 
Sbjct: 231979 YSSTSRQMSGTSWKAAFPDIHYVIFLCRATVFLFEPS*AGNRRS*LRCRKSSGAAGGLF* 232158

Query: 107    G 107
              G
Sbjct: 232159 G 232161


>gb|AAQM02000637.1| Toxoplasma gondii GT1 gcontig_1107000829554, whole genome shotgun
            sequence
          Length = 2392

 Score = 28.1 bits (61), Expect = 3.3
 Identities = 14/31 (45%), Positives = 20/31 (64%), Gaps = 2/31 (6%)
 Frame = +3

Query: 71   SLPDAITFHSDPSIPGRPRTEIC--CKKCGG 99
            SLPD  T  + PS+ G PR ++C   ++CGG
Sbjct: 1014 SLPD--TNGASPSVDGLPRLKVCSPVRRCGG 1100


>gb|AAQM02000123.1| Toxoplasma gondii GT1 gcontig_1107000946696, whole genome shotgun
              sequence
          Length = 194678

 Score = 28.1 bits (61), Expect = 3.3
 Identities = 17/56 (30%), Positives = 29/56 (51%), Gaps = 3/56 (5%)
 Frame = +2

Query: 6      EQEWRVKLSPEQFRVLREKGTEAP--FVGKYTDNKDEG-LYECAGCGTPLFKSTQK 58
              E+ W+ + +PE F  L+E G  +P    G +  NK EG  +      +P+ + T+K
Sbjct: 193460 EKLWKTQSAPESFFCLQETGAGSPTSLFGFFGRNKTEGSAFLSCELSSPVRRCTRK 193627


>gb|AAQM02000035.1| Toxoplasma gondii GT1 gcontig_1107000925766, whole genome shotgun
             sequence
          Length = 408006

 Score = 28.1 bits (61), Expect = 3.3
 Identities = 11/14 (78%), Positives = 11/14 (78%)
 Frame = -3

Query: 89    RTEICCKKCGGHLG 102
             RT IC KK GGHLG
Sbjct: 10789 RTTICAKKVGGHLG 10748


>gb|AAQM02000022.1| Toxoplasma gondii GT1 gcontig_1107000925796, whole genome shotgun
              sequence
          Length = 496205

 Score = 28.1 bits (61), Expect = 3.3
 Identities = 10/15 (66%), Positives = 11/15 (73%)
 Frame = -1

Query: 59     FHSGCGWPSFFDSLP 73
              FH  C WPSFF S+P
Sbjct: 322208 FHRCCFWPSFFPSVP 322164


>gb|AAQM02000183.1| Toxoplasma gondii GT1 gcontig_1107000996866, whole genome shotgun
             sequence
          Length = 101307

 Score = 27.7 bits (60), Expect = 4.3
 Identities = 14/45 (31%), Positives = 19/45 (42%)
 Frame = -1

Query: 55    STQKFHSGCGWPSFFDSLPDAITFHSDPSIPGRPRTEICCKKCGG 99
             ST+  H  C   +   S  D     SDPS+    R  + C +C G
Sbjct: 49797 STRCCHDCCTPETHLSSREDMFEKDSDPSVLDEARCSLRCPRCSG 49663


>gb|AAQM02000277.1| Toxoplasma gondii GT1 gcontig_1107000974560, whole genome shotgun
            sequence
          Length = 30840

 Score = 27.3 bits (59), Expect = 5.6
 Identities = 12/30 (40%), Positives = 17/30 (56%)
 Frame = +3

Query: 70   DSLPDAITFHSDPSIPGRPRTEICCKKCGG 99
            +S+P  +T HSDP+    P+ E  CK   G
Sbjct: 7107 NSVPRQMTSHSDPTEKHDPQFETFCKAASG 7196



 Score = 27.3 bits (59), Expect = 5.6
 Identities = 12/30 (40%), Positives = 17/30 (56%)
 Frame = +2

Query: 70    DSLPDAITFHSDPSIPGRPRTEICCKKCGG 99
             +S+P  +T HSDP+    P+ E  CK   G
Sbjct: 20327 NSVPRQMTSHSDPTEKHDPQFETFCKAASG 20416


>gb|AAQM02000027.1| Toxoplasma gondii GT1 gcontig_1107000955098, whole genome shotgun
             sequence
          Length = 469463

 Score = 27.3 bits (59), Expect = 5.6
 Identities = 16/47 (34%), Positives = 23/47 (48%), Gaps = 3/47 (6%)
 Frame = -2

Query: 51    PLFKSTQKFHSGCGWPSF---FDSLPDAITFHSDPSIPGRPRTEICC 94
             PL  +   F S    PSF   F++L  A+  H  PS+     T++CC
Sbjct: 18946 PLATAADGFSSTPKAPSFLEMFNALAPAVDSHLAPSLLQESFTQVCC 18806


>gb|AAQM02000070.1| Toxoplasma gondii GT1 gcontig_1107000852474, whole genome shotgun
              sequence
          Length = 302048

 Score = 26.6 bits (57), Expect = 9.5
 Identities = 15/42 (35%), Positives = 19/42 (45%)
 Frame = +1

Query: 52     LFKSTQKFHSGCGWPSFFDSLPDAITFHSDPSIPGRPRTEIC 93
              LF S++K      WP         + FH D S+PG  RT  C
Sbjct: 112981 LFLSSRKEERLLAWP---------VVFHLDLSLPGTLRTLFC 113079


>gb|AAQM02000054.1| Toxoplasma gondii GT1 gcontig_1107000855630, whole genome shotgun
              sequence
          Length = 335650

 Score = 26.6 bits (57), Expect = 9.5
 Identities = 12/40 (30%), Positives = 18/40 (45%)
 Frame = +1

Query: 73     PDAITFHSDPSIPGRPRTEICCKKCGGHLGHVFKGEGFNT 112
              P  +     PS+P    + I C++   + G  F GE F T
Sbjct: 179596 PSVLVSSPVPSLPTYGSSAIGCRRYSANSGRRFSGESFET 179715


>gb|AAQM02000050.1| Toxoplasma gondii GT1 gcontig_1107000845212, whole genome shotgun
             sequence
          Length = 365206

 Score = 26.6 bits (57), Expect = 9.5
 Identities = 17/46 (36%), Positives = 21/46 (45%), Gaps = 2/46 (4%)
 Frame = -3

Query: 49    GTPLFKSTQKFHSG--CGWPSFFDSLPDAITFHSDPSIPGRPRTEI 92
             GTP   ++QK  S   C W S          FHS P+ P   RTE+
Sbjct: 63758 GTPTQGASQKERSVHLCSWVSSALREHSGHLFHSHPTTPLEARTEV 63621


>gb|AAQM02000041.1| Toxoplasma gondii GT1 gcontig_1107000869098, whole genome shotgun
             sequence
          Length = 388859

 Score = 26.6 bits (57), Expect = 9.5
 Identities = 17/55 (30%), Positives = 24/55 (43%)
 Frame = +3

Query: 44    ECAGCGTPLFKSTQKFHSGCGWPSFFDSLPDAITFHSDPSIPGRPRTEICCKKCG 98
             +C G      KS +   +G   P FF   PD +   S   + G PR  + C+ CG
Sbjct: 97848 DCLGVSVGALKSPRGVDNG---PRFF---PDVLLPSSRVVLRGDPRHCVRCRSCG 97994


>gb|AAQM02000018.1| Toxoplasma gondii GT1 gcontig_1107000879894, whole genome shotgun
              sequence
          Length = 524503

 Score = 26.6 bits (57), Expect = 9.5
 Identities = 20/88 (22%), Positives = 34/88 (38%), Gaps = 5/88 (5%)
 Frame = -1

Query: 15     PEQFRVLREKGTEAPFVGKYTDNKDEGLYECAGCGTPLFKSTQKFHSGCGWPSFFDSLPD 74
              P     ++ +G + P        +DEG  E       +  +   F + C +   FDS P 
Sbjct: 173554 PVDVEAIKVRGPQLP------GERDEGRLELPAFAISVAAAPANFGALCTFADAFDSFPG 173393

Query: 75     AITFHSDP---SIPG--RPRTEICCKKC 97
               I    +    S+ G  RP++   C +C
Sbjct: 173392 GICLGGEVQALSLAGSFRPQSRHVCARC 173309


>gb|AAQM02000009.1| Toxoplasma gondii GT1 gcontig_1107000925794, whole genome shotgun
              sequence
          Length = 613615

 Score = 26.6 bits (57), Expect = 9.5
 Identities = 16/41 (39%), Positives = 22/41 (53%), Gaps = 1/41 (2%)
 Frame = +1

Query: 8      EWRVKL-SPEQFRVLREKGTEAPFVGKYTDNKDEGLYECAG 47
              E RV L    +F VLRE+G+      +YT  +D  L +C G
Sbjct: 196636 EARVSLVQTTRFLVLRERGSRKACHRRYTRQRDWHLLKCIG 196758


  Database: genome.fa
    Posted date:  Jan 16, 2009 12:51 PM
  Number of letters in database: 66,210,174
  Number of sequences in database:  3590
  
Lambda     K      H
   0.319    0.139    0.457 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,981,347
Number of Sequences: 3590
Number of extensions: 281648
Number of successful extensions: 1505
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 494
Number of HSP's successfully gapped in prelim test: 54
Number of HSP's that attempted gapping in prelim test: 532
Number of HSP's gapped (non-prelim): 1294
length of query: 132
length of database: 22,070,058
effective HSP length: 93
effective length of query: 39
effective length of database: 21,736,188
effective search space: 847711332
effective search space used: 847711332
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)
