TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= scaffold_27 dpurpureum selr protein
         (132 letters)

Database: genome.fa 
           113 sequences; 29,453,142 total letters

Searching........................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAFD02000006.1| Thalassiosira pseudonana CCMP1335 chromosome ...    96   6e-21
gb|AAFD02000004.1| Thalassiosira pseudonana CCMP1335 chromosome ...    74   3e-14
gb|AAFD02000003.1| Thalassiosira pseudonana CCMP1335 chromosome ...    38   0.002
gb|AAFD02000010.1| Thalassiosira pseudonana CCMP1335 chromosome ...    28   1.7  
gb|AAFD02000036.1| Thalassiosira pseudonana CCMP1335 chromosome ...    27   2.8  
gb|AAFD02000015.1| Thalassiosira pseudonana CCMP1335 chromosome ...    26   6.3  
gb|AAFD02000012.1| Thalassiosira pseudonana CCMP1335 chromosome ...    26   6.3  
gb|AAFD02000011.1| Thalassiosira pseudonana CCMP1335 chromosome ...    26   6.3  

>gb|AAFD02000006.1| Thalassiosira pseudonana CCMP1335 chromosome 4 THAPSchr_4_Cont6, whole
              genome shotgun sequence
          Length = 1267202

 Score = 95.9 bits (237), Expect = 6e-21
 Identities = 54/145 (37%), Positives = 76/145 (52%), Gaps = 18/145 (12%)
 Frame = +3

Query: 6      EQEWRVKLSPEQFRVLREKGTEAPFVGKYTDNKDEGLYECAGCGTPLFKSTQKFHSGCGW 65
              E EW   LS +Q+ +LR+ GTE+P+       +  G+Y CAGCGTPLF  +QKFHSG GW
Sbjct: 888906 ESEWNEMLSKQQYFILRQGGTESPYSSILEAEERPGVYACAGCGTPLFDGSQKFHSGTGW 889085

Query: 66     PSFFDSLPDAITFHSDPSIPG------------RPRTEICCKKCGGHLGHVFKGEGF--- 110
              PSF       +   ++P +                  E+ C  CGGHLG VF  +G+   
Sbjct: 889086 PSF---ATPVMNDSANPKVSNVEMEDVSQIQYQLAGAEVRCHSCGGHLGDVF-ADGYLFV 889253

Query: 111    NTPI---DQRHCVNSISLNFKPSKQ 132
               TP     +R+C++  +L F P +Q
Sbjct: 889254 GTPAFISGKRYCIDGGALVFVPKEQ 889328


>gb|AAFD02000004.1| Thalassiosira pseudonana CCMP1335 chromosome 2 THAPSchr_2_Cont4, whole
              genome shotgun sequence
          Length = 2234390

 Score = 73.6 bits (179), Expect = 3e-14
 Identities = 39/92 (42%), Positives = 49/92 (53%)
 Frame = +1

Query: 14     SPEQFRVLREKGTEAPFVGKYTDNKDEGLYECAGCGTPLFKSTQKFHSGCGWPSFFDSLP 73
              +P    VL+   TE  +   Y  NK  G+Y CA C +PLF S  K+ SG GWPSF+ ++ 
Sbjct: 349948 NPLSLAVLKLGFTEPAWSSLYNYNKSSGIYSCANCRSPLFSSAAKYDSGSGWPSFWRTV- 350124

Query: 74     DAITFHSDPSIPGRPRTEICCKKCGGHLGHVF 105
              D+          G  R E  C  CGGHLGHVF
Sbjct: 350125 DSERVSLKKEWDG--RIECLCANCGGHLGHVF 350214


>gb|AAFD02000003.1| Thalassiosira pseudonana CCMP1335 chromosome 2 THAPSchr_2_Cont3, whole
              genome shotgun sequence
          Length = 472755

 Score = 38.1 bits (87), Expect = 0.002
 Identities = 41/149 (27%), Positives = 62/149 (41%), Gaps = 20/149 (13%)
 Frame = -3

Query: 1      MSDLNEQEWRVKLSPEQFRVLREKGTEAPFVGKYTDNKDE--------------GLYECA 46
              M  +N   +   +S +  +VL E GT+ P V K T+N  +              G++ CA
Sbjct: 170725 MESINNNNY---VSEQAKKVLLEGGTDPPHV-KLTNNGYDFSTNHLQSLHFPHVGVFTCA 170558

Query: 47     GCGTPLFKSTQKFHS----GCGWPSFFDSLPDAITFHSDPSIPGRP-RTEICCKKCGGHL 101
                  PLF +     S      GW SF   +         P++     R E+   K G HL
Sbjct: 170557 VGLMPLFTTADLSQSISTASSGWLSFKSVISQDHIVLVHPTLNDLDQRIEVLDAKSGCHL 170378

Query: 102    GHVF-KGEGFNTPIDQRHCVNSISLNFKP 129
              GH F + +G+        C+N+ +LNF P
Sbjct: 170377 GHYFGRVDGY--------CINASALNFTP 170315


>gb|AAFD02000010.1| Thalassiosira pseudonana CCMP1335 chromosome 6 THAPSchr_6_Cont10, whole
               genome shotgun sequence
          Length = 2071480

 Score = 28.1 bits (61), Expect = 1.7
 Identities = 11/31 (35%), Positives = 18/31 (58%)
 Frame = +3

Query: 64      GWPSFFDSLPDAITFHSDPSIPGRPRTEICC 94
               G+P ++D+L D +   +  +  GR  TE CC
Sbjct: 1182255 GYPGYYDALTDTVVQAAFANTRGRVDTEGCC 1182347



 Score = 26.2 bits (56), Expect = 6.3
 Identities = 7/19 (36%), Positives = 13/19 (68%)
 Frame = -1

Query: 47      GCGTPLFKSTQKFHSGCGW 65
               G G+ +++  +K+HS C W
Sbjct: 1740175 GAGSDVYERRRKYHSACAW 1740119



 Score = 25.8 bits (55), Expect = 8.2
 Identities = 10/19 (52%), Positives = 14/19 (73%)
 Frame = +3

Query: 66      PSFFDSLPDAITFHSDPSI 84
               PSF  S+P  I+FHS P++
Sbjct: 1332234 PSFLLSIPFLISFHSSPAL 1332290


>gb|AAFD02000036.1| Thalassiosira pseudonana CCMP1335 chromosome 20 THAPSchr_20_Cont36, whole
              genome shotgun sequence
          Length = 621618

 Score = 27.3 bits (59), Expect = 2.8
 Identities = 14/47 (29%), Positives = 22/47 (46%)
 Frame = -3

Query: 49     GTPLFKSTQKFHSGCGWPSFFDSLPDAITFHSDPSIPGRPRTEICCK 95
              G PL  +   FH+   WPS++  L   + ++  P     PR   CC+
Sbjct: 465109 GNPLEDTPHIFHTSSSWPSYY-CLVSPLRYY*RPM--*EPRVHCCCQ 464978


>gb|AAFD02000015.1| Thalassiosira pseudonana CCMP1335 chromosome 11a THAPSchr_11a_Cont15,
              whole genome shotgun sequence
          Length = 761890

 Score = 26.2 bits (56), Expect = 6.3
 Identities = 16/53 (30%), Positives = 22/53 (41%)
 Frame = -1

Query: 45     CAGCGTPLFKSTQKFHSGCGWPSFFDSLPDAITFHSDPSIPGRPRTEICCKKC 97
              C    TP F  +Q   + C +PS   S+P +I        P  P   I C +C
Sbjct: 222313 CVATSTPPFSYSQ---APCRYPSCCQSIPHSIQPRHVDHRPL*PSVSIACFQC 222164


>gb|AAFD02000012.1| Thalassiosira pseudonana CCMP1335 chromosome 9 THAPSchr_9_Cont12, whole
              genome shotgun sequence
          Length = 1191060

 Score = 26.2 bits (56), Expect = 6.3
 Identities = 13/32 (40%), Positives = 14/32 (43%)
 Frame = -1

Query: 71     SLPDAITFHSDPSIPGRPRTEICCKKCGGHLG 102
              SLP     H  PS P  P +E  C     HLG
Sbjct: 987174 SLPRLSPDHMSPSCPTMPTSEFLCSIAFMHLG 987079



 Score = 25.8 bits (55), Expect = 8.2
 Identities = 13/32 (40%), Positives = 14/32 (43%)
 Frame = -1

Query: 71     SLPDAITFHSDPSIPGRPRTEICCKKCGGHLG 102
              SLP     H  PS P  P +E  C     HLG
Sbjct: 991413 SLPRLSPDHMSPSCPTMPTSEFLCSIAFIHLG 991318


>gb|AAFD02000011.1| Thalassiosira pseudonana CCMP1335 chromosome 8 THAPSchr_8_Cont11, whole
              genome shotgun sequence
          Length = 1267198

 Score = 26.2 bits (56), Expect = 6.3
 Identities = 14/38 (36%), Positives = 16/38 (42%)
 Frame = -2

Query: 77     TFHSDPSIPGRPRTEICCKKCGGHLGHVFKGEGFNTPI 114
              +F  D  +P  P    CC KC    GHV   E    PI
Sbjct: 578265 SFVID*FVPSSPT--FCCTKCIVRCGHVMYAERIEQPI 578158


  Database: genome.fa
    Posted date:  Jan 19, 2010  5:14 PM
  Number of letters in database: 29,453,142
  Number of sequences in database:  113
  
Lambda     K      H
   0.319    0.139    0.457 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,312,968
Number of Sequences: 113
Number of extensions: 110611
Number of successful extensions: 520
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 479
Number of HSP's gapped (non-prelim): 121
length of query: 132
length of database: 9,817,714
effective HSP length: 88
effective length of query: 44
effective length of database: 9,807,770
effective search space: 431541880
effective search space used: 431541880
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)
