TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= scaffold_27 dpurpureum selr protein
         (132 letters)

Database: genome.fa 
           218 sequences; 41,633,360 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_12                                                            67   3e-12
scaffold_9                                                             30   0.59 
scaffold_3                                                             30   0.59 
scaffold_28                                                            30   0.77 
scaffold_54                                                            29   1.0  
scaffold_11                                                            29   1.0  
scaffold_35                                                            28   2.2  
scaffold_20                                                            28   2.2  
scaffold_27                                                            28   2.9  
scaffold_18                                                            28   2.9  
scaffold_49                                                            27   3.8  
scaffold_38                                                            27   3.8  
scaffold_33                                                            27   3.8  
scaffold_19                                                            27   3.8  
scaffold_2                                                             27   3.8  
scaffold_37                                                            27   5.0  
scaffold_23                                                            27   5.0  
scaffold_15                                                            27   5.0  
scaffold_7                                                             27   5.0  
scaffold_6                                                             27   5.0  
scaffold_4                                                             27   5.0  
scaffold_32                                                            27   6.5  
scaffold_14                                                            27   6.5  
scaffold_10                                                            27   6.5  
scaffold_29                                                            26   8.5  
scaffold_25                                                            26   8.5  
scaffold_16                                                            26   8.5  

>scaffold_12 
          Length = 1128436

 Score = 67.4 bits (163), Expect = 3e-12
 Identities = 59/217 (27%), Positives = 75/217 (34%), Gaps = 102/217 (47%)
 Frame = -1

Query: 13     LSPEQFRVLREKGTE-----------APFV-----------------------GKYTD-N 37
              LS E++RVLR KGTE           +P V                       G+Y    
Sbjct: 514624 LSKEEYRVLRNKGTEPTNGVSNDAFFSPLVN*VSR*DAVCWGYLKASDAFCGLGEYDKFY 514445

Query: 38     KDEGLYECAGCGTPLFKSTQKFH-----------------------------SGCGWPSF 68
                +G + C GC TPL+ +  KF                              SGCGWP+F
Sbjct: 514444 PKKGYFVCRGCQTPLYSAAAKFARLVF*WATVRA*L*AEVCASRMGCCRVPISGCGWPAF 514265

Query: 69     FDSLPDAITFHSDPSIP--------------------------------------GRPRT 90
                    A+  H D S                                        G  R 
Sbjct: 514264 DKCYAGAVKTHVDNSFGMCIHHASMLYLPARIDCGRAIL*SVSPHDLFAHLT*YLGMRRV 514085

Query: 91     EICCKKCGGHLGHVFKGEGFNTPIDQRHCVNSISLNF 127
              EI C  C GHLGHVF+GE F T  ++RHCVNS+S+ +
Sbjct: 514084 EIVCAACDGHLGHVFEGERF-TETNERHCVNSVSVRY 513977



 Score = 26.6 bits (57), Expect = 6.5
 Identities = 11/28 (39%), Positives = 15/28 (53%)
 Frame = +3

Query: 82      PSIPGRPRTEICCKKCGGHLGHVFKGEG 109
               P   GR R+ + C   G HLG+   G+G
Sbjct: 1118319 PGFEGRWRS*LLCNAAGAHLGNSRAGQG 1118402



 Score = 26.2 bits (56), Expect = 8.5
 Identities = 10/35 (28%), Positives = 16/35 (45%)
 Frame = -1

Query: 93     CCKKCGGHLGHVFKGEGFNTPIDQRHCVNSISLNF 127
              CC+  GGH  H       + P+++R   N  +  F
Sbjct: 816016 CCQVHGGHQHHYQPSSAMH*PVEKRRAANENTKGF 815912


>scaffold_9 
          Length = 1303534

 Score = 30.0 bits (66), Expect = 0.59
 Identities = 13/30 (43%), Positives = 16/30 (53%)
 Frame = +1

Query: 66     PSFFDSLPDAITFHSDPSIPGRPRTEICCK 95
              PS  D  PDA   H+ P +PG PR  +  K
Sbjct: 689029 PSMTDVRPDAAPTHNQPLLPGTPRALLVLK 689118



 Score = 26.9 bits (58), Expect = 5.0
 Identities = 10/19 (52%), Positives = 15/19 (78%)
 Frame = +3

Query: 67     SFFDSLPDAITFHSDPSIP 85
              S+F  + DA+TFH+D S+P
Sbjct: 155514 SYFALVVDAVTFHADLSVP 155570



 Score = 26.6 bits (57), Expect = 6.5
 Identities = 11/26 (42%), Positives = 13/26 (50%)
 Frame = +3

Query: 78     FHSDPSIPGRPRTEICCKKCGGHLGH 103
              FH  P+   RPR   C +   GH GH
Sbjct: 742047 FHGQPATSRRPRRHACRRCSLGHDGH 742124


>scaffold_3 
          Length = 2609234

 Score = 30.0 bits (66), Expect = 0.59
 Identities = 21/74 (28%), Positives = 31/74 (41%)
 Frame = -2

Query: 21     LREKGTEAPFVGKYTDNKDEGLYECAGCGTPLFKSTQKFHSGCGWPSFFDSLPDAITFHS 80
              L +    +PF  K+T  ++E   EC+   TP   S    H  C  P  +  +   +T  S
Sbjct: 657478 LNKYSVPSPFCHKFTHPQEEPHQECSWHMTPFATSV---HDMCLLPELYSQIKSVLT--S 657314

Query: 81     DPSIPGRPRTEICC 94
               P + G     ICC
Sbjct: 657313 PPHVSG---LGICC 657281



 Score = 27.7 bits (60), Expect = 2.9
 Identities = 16/49 (32%), Positives = 22/49 (44%)
 Frame = -1

Query: 79      HSDPSIPGRPRTEICCKKCGGHLGHVFKGEGFNTPIDQRHCVNSISLNF 127
               +S  S  G PR   CC    G+L H+  G G  +      CV+  +L F
Sbjct: 1496426 YSSTSCRGTPRFCTCC*VLQGYLSHLLTGRGTASEPVYLTCVSLQALYF 1496280



 Score = 26.9 bits (58), Expect = 5.0
 Identities = 9/13 (69%), Positives = 11/13 (84%)
 Frame = +3

Query: 85     PGRPRTEICCKKC 97
              PGRPRT IC ++C
Sbjct: 579954 PGRPRTRICPRRC 579992


>scaffold_28 
          Length = 614053

 Score = 29.6 bits (65), Expect = 0.77
 Identities = 18/55 (32%), Positives = 26/55 (47%), Gaps = 4/55 (7%)
 Frame = -3

Query: 42     LYECAGCGTPLFKSTQKFHSGCGWPSF----FDSLPDAITFHSDPSIPGRPRTEI 92
              L+ CA  G  + KS +  H   G  +     +  L D     + PS+PGRPRT +
Sbjct: 376019 LHVCARHGEEMVKSARGTHEQAGAKATREETYHHLLDLRLAGAPPSLPGRPRTSV 375855


>scaffold_54 
          Length = 119841

 Score = 29.3 bits (64), Expect = 1.0
 Identities = 18/47 (38%), Positives = 24/47 (51%)
 Frame = +3

Query: 67     SFFDSLPDAITFHSDPSIPGRPRTEICCKKCGGHLGHVFKGEGFNTP 113
              +FF SL   I+ HS   +PG P+   C + C     H+ KGE F  P
Sbjct: 100581 TFFFSLRSRISPHSRQRVPG*PK-YTCPRNCQPR--HLAKGESFAAP 100712


>scaffold_11 
          Length = 1136692

 Score = 29.3 bits (64), Expect = 1.0
 Identities = 29/99 (29%), Positives = 38/99 (38%), Gaps = 11/99 (11%)
 Frame = +1

Query: 44     ECAGCGTPLFKSTQKFHSGCGWPSFFDSLPDAITFHSDPSIPGRP-----RTEICCKKCG 98
              + A  G   F S++   S C WP      P  +   SDP  P  P       E+     G
Sbjct: 902701 DAASPGAAPFTSSE---SAC-WPC-----PAVVKSSSDPLSPSSPDDCASSAELAMVPLG 902853

Query: 99     GHLGHV------FKGEGFNTPIDQRHCVNSISLNFKPSK 131
               + GHV         EGF+ P  Q H      +N KP+K
Sbjct: 902854 PNQGHVRPRRTTASEEGFSHPTVQSHPSTGRYMNRKPAK 902970


>scaffold_35 
          Length = 432256

 Score = 28.1 bits (61), Expect = 2.2
 Identities = 13/38 (34%), Positives = 20/38 (52%)
 Frame = +3

Query: 95     KKCGGHLGHVFKGEGFNTPIDQRHCVNSISLNFKPSKQ 132
              + CG  +G  F   G       R CV+++ L+ KP+KQ
Sbjct: 397362 RACGADVGRHFGSRG------HRRCVDAMELSIKPAKQ 397457


>scaffold_20 
          Length = 720398

 Score = 28.1 bits (61), Expect = 2.2
 Identities = 19/45 (42%), Positives = 20/45 (44%), Gaps = 8/45 (17%)
 Frame = +3

Query: 64     GW---PSFFDSLPDAITFHSDPS-----IPGRPRTEICCKKCGGH 100
              GW   PSFF SLP A   HS PS         PR   C + C  H
Sbjct: 125277 GWAALPSFFFSLPPARPLHSLPSPHTYHQAQSPRDPRC*RHCNTH 125411


>scaffold_27 
          Length = 606668

 Score = 27.7 bits (60), Expect = 2.9
 Identities = 11/27 (40%), Positives = 15/27 (55%)
 Frame = +3

Query: 73     PDAITFHSDPSIPGRPRTEICCKKCGG 99
              PDA+    +   P R +  +CC KCGG
Sbjct: 283800 PDAVAQAWEYQQPPRSKGSLCCIKCGG 283880


>scaffold_18 
          Length = 855359

 Score = 27.7 bits (60), Expect = 2.9
 Identities = 19/66 (28%), Positives = 25/66 (37%), Gaps = 10/66 (15%)
 Frame = +3

Query: 62     GCGWPSFFDSLP----------DAITFHSDPSIPGRPRTEICCKKCGGHLGHVFKGEGFN 111
              GC WP+   S P          D  +  +DPS   RP +         H  H F G+   
Sbjct: 258765 GCAWPAACSSAPNAPDRSSS**DCCSRSTDPSARARPGS-------SAHARHSFAGKEPA 258923

Query: 112    TPIDQR 117
              TP + R
Sbjct: 258924 TPSEPR 258941


>scaffold_49 
          Length = 198086

 Score = 27.3 bits (59), Expect = 3.8
 Identities = 10/17 (58%), Positives = 11/17 (64%)
 Frame = -3

Query: 93     CCKKCGGHLGHVFKGEG 109
              C  +CG   GHV KGEG
Sbjct: 123693 CVSRCGHACGHVHKGEG 123643


>scaffold_38 
          Length = 375890

 Score = 27.3 bits (59), Expect = 3.8
 Identities = 19/63 (30%), Positives = 25/63 (39%), Gaps = 3/63 (4%)
 Frame = +3

Query: 43     YECAGCGTPLFKST--QKFHSGCGWPSFFD-SLPDAITFHSDPSIPGRPRTEICCKKCGG 99
              + C  CGT +  S    K   G  W      +L       S+PS    P  + CC KC  
Sbjct: 263247 FGCYCCGTKIVDSATIHKMKGGSVWTKGKPLTLRPYGETRSNPSKNNTPYQQACCAKCNF 263426

Query: 100    HLG 102
              +LG
Sbjct: 263427 NLG 263435


>scaffold_33 
          Length = 480061

 Score = 27.3 bits (59), Expect = 3.8
 Identities = 12/39 (30%), Positives = 19/39 (48%)
 Frame = -1

Query: 61     SGCGWPSFFDSLPDAITFHSDPSIPGRPRTEICCKKCGG 99
              S C +P    + P+AI    D + P +P+ E   +K  G
Sbjct: 214831 SACVYPETLQASPEAICLQEDQAYPAQPQDEYGREKLRG 214715


>scaffold_19 
          Length = 781963

 Score = 27.3 bits (59), Expect = 3.8
 Identities = 9/21 (42%), Positives = 14/21 (66%)
 Frame = +1

Query: 39     DEGLYECAGCGTPLFKSTQKF 59
              ++G Y C+ C  PL+ +T KF
Sbjct: 592771 EDGAYRCSRCQQPLYDATAKF 592833


>scaffold_2 
          Length = 3607471

 Score = 27.3 bits (59), Expect = 3.8
 Identities = 8/16 (50%), Positives = 12/16 (75%)
 Frame = -2

Query: 82      PSIPGRPRTEICCKKC 97
               P++P RP+  ICC+ C
Sbjct: 2600577 PALPHRPQVPICCRHC 2600530


>scaffold_37 
          Length = 404957

 Score = 26.9 bits (58), Expect = 5.0
 Identities = 9/14 (64%), Positives = 10/14 (71%)
 Frame = -3

Query: 52     LFKSTQKFHSGCGW 65
              L K +Q FHSGC W
Sbjct: 315756 LIKCSQSFHSGCSW 315715


>scaffold_23 
          Length = 690264

 Score = 26.9 bits (58), Expect = 5.0
 Identities = 24/77 (31%), Positives = 36/77 (46%), Gaps = 7/77 (9%)
 Frame = -2

Query: 61    SGCGWPSFFDSLPDAITFHSDPSIPGRPRTEICCKKCGGHLGHVFKGEGFNTPI-----D 115
             SGC   S F+S   ++     P    +   +I CKK GG+L HV    G  T +      
Sbjct: 83681 SGC---SSFNSRSSSV-----PQQRSKENCQIKCKKQGGNLAHVCT*SGLETMLLAVRAA 83526

Query: 116   QRHCVNSIS--LNFKPS 130
              R+C + ++  L F+PS
Sbjct: 83525 GRNCAH*LTAYLLFEPS 83475


>scaffold_15 
          Length = 1049915

 Score = 26.9 bits (58), Expect = 5.0
 Identities = 20/70 (28%), Positives = 28/70 (40%)
 Frame = -3

Query: 42     LYECAGCGTPLFKSTQKFHSGCGWPSFFDSLPDAITFHSDPSIPGRPRTEICCKKCGGHL 101
              L++   CG+   +   +    C WP F     D   + S P +  R   + CC KCG   
Sbjct: 995472 LWDL*PCGSSCGQIENRELVSC-WPEF-----DMSPWQSTPGLTLRD*QQRCCGKCGSD- 995314

Query: 102    GHVFKGEGFN 111
              G    G G N
Sbjct: 995313 GRAPSGSGSN 995284



 Score = 26.2 bits (56), Expect = 8.5
 Identities = 12/24 (50%), Positives = 13/24 (54%)
 Frame = +3

Query: 86    GRPRTEICCKKCGGHLGHVFKGEG 109
             GRPR  I  K C   LGH  +G G
Sbjct: 83115 GRPRWRIQSKTC*NRLGHAQEGGG 83186


>scaffold_7 
          Length = 1435906

 Score = 26.9 bits (58), Expect = 5.0
 Identities = 11/35 (31%), Positives = 17/35 (48%)
 Frame = -1

Query: 61     SGCGWPSFFDSLPDAITFHSDPSIPGRPRTEICCK 95
              +GCG      S+PD    H  P++P      +CC+
Sbjct: 560101 NGCGVDEQHASMPDIHRKHVRPTMPPCTHAHLCCQ 559997


>scaffold_6 
          Length = 1473069

 Score = 26.9 bits (58), Expect = 5.0
 Identities = 17/48 (35%), Positives = 22/48 (45%), Gaps = 3/48 (6%)
 Frame = -2

Query: 75      AITFHS---DPSIPGRPRTEICCKKCGGHLGHVFKGEGFNTPIDQRHC 119
               A+T HS   +P IPGRP  +  C      LGH    +   T   + HC
Sbjct: 1385810 ALTNHSTNDNPFIPGRPHQQK*CLAPAAVLGHPCAADA-GTKSPEEHC 1385670



 Score = 26.6 bits (57), Expect = 6.5
 Identities = 11/21 (52%), Positives = 13/21 (61%)
 Frame = -1

Query: 83      SIPGRPRTEICCKKCGGHLGH 103
               S+P RP T +CCK    HL H
Sbjct: 1019208 SVPHRPNTRLCCK----HLTH 1019158



 Score = 26.6 bits (57), Expect = 6.5
 Identities = 15/38 (39%), Positives = 18/38 (47%)
 Frame = +3

Query: 51     PLFKSTQKFHSGCGWPSFFDSLPDAITFHSDPSIPGRP 88
              PL +STQ    GCGW     +L      H DP +P  P
Sbjct: 895437 PLGQSTQAVEPGCGW-----ALASLHAVH-DPPVPAVP 895532


>scaffold_4 
          Length = 2081438

 Score = 26.9 bits (58), Expect = 5.0
 Identities = 12/38 (31%), Positives = 18/38 (47%), Gaps = 2/38 (5%)
 Frame = +3

Query: 62      GCGWPSFFDSLP--DAITFHSDPSIPGRPRTEICCKKC 97
               GC  P+  D+     A +  + P  P  P  + CCK+C
Sbjct: 1655532 GCAKPASADTATRQSATSVRAMPPAPSTPTPKTCCKQC 1655645


>scaffold_32 
          Length = 547532

 Score = 26.6 bits (57), Expect = 6.5
 Identities = 11/28 (39%), Positives = 15/28 (53%)
 Frame = -1

Query: 82   PSIPGRPRTEICCKKCGGHLGHVFKGEG 109
            P   GR R+ + C   G HLG+   G+G
Sbjct: 4109 PGFEGRWRS*LLCNAAGAHLGNSRAGQG 4026


>scaffold_14 
          Length = 1073601

 Score = 26.6 bits (57), Expect = 6.5
 Identities = 21/75 (28%), Positives = 25/75 (33%)
 Frame = -1

Query: 36     DNKDEGLYECAGCGTPLFKSTQKFHSGCGWPSFFDSLPDAITFHSDPSIPGRPRTEICCK 95
              D  D G     GCG            GCG+    DS  D +  H       R R   CC 
Sbjct: 292755 DGGDVGCDRGCGCGCG-----HDCDCGCGF--VHDSGRDLVHVHGHGHSDARARHHCCCC 292597

Query: 96     KCGGHLGHVFKGEGF 110
                 GH+      +GF
Sbjct: 292596 YGCGHVPRSSSADGF 292552


>scaffold_10 
          Length = 1258938

 Score = 26.6 bits (57), Expect = 6.5
 Identities = 11/28 (39%), Positives = 15/28 (53%)
 Frame = +3

Query: 82      PSIPGRPRTEICCKKCGGHLGHVFKGEG 109
               P   GR R+ + C   G HLG+   G+G
Sbjct: 1254264 PGFEGRWRS*LLCNAAGAHLGNSRAGQG 1254347


>scaffold_29 
          Length = 584948

 Score = 26.2 bits (56), Expect = 8.5
 Identities = 12/29 (41%), Positives = 16/29 (55%)
 Frame = +1

Query: 94     CKKCGGHLGHVFKGEGFNTPIDQRHCVNS 122
              C  CGG  GH F+G G    + +R C +S
Sbjct: 511654 CHACGG-CGHQFRGWGAFACLSRRACASS 511737


>scaffold_25 
          Length = 667690

 Score = 26.2 bits (56), Expect = 8.5
 Identities = 14/45 (31%), Positives = 18/45 (40%)
 Frame = -1

Query: 54     KSTQKFHSGCGWPSFFDSLPDAITFHSDPSIPGRPRTEICCKKCG 98
              + T++ H  CGWP              D  IP R +  IC   CG
Sbjct: 255208 RPTRRNHHRCGWP------------FPDHQIPRRHQRAICPLACG 255110


>scaffold_16 
          Length = 929013

 Score = 26.2 bits (56), Expect = 8.5
 Identities = 10/25 (40%), Positives = 14/25 (56%)
 Frame = +1

Query: 73     PDAITFHSDPSIPGRPRTEICCKKC 97
              P A   H+DPS+P  P    C ++C
Sbjct: 437716 PGARRKHADPSLPADPMPVDCSQRC 437790


  Database: genome.fa
    Posted date:  Jan 19, 2010  5:06 PM
  Number of letters in database: 41,633,360
  Number of sequences in database:  218
  
Lambda     K      H
   0.319    0.139    0.457 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,996,464
Number of Sequences: 218
Number of extensions: 197232
Number of successful extensions: 1202
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 38
Number of HSP's successfully gapped in prelim test: 17
Number of HSP's that attempted gapping in prelim test: 892
Number of HSP's gapped (non-prelim): 639
length of query: 132
length of database: 13,877,786
effective HSP length: 90
effective length of query: 42
effective length of database: 13,858,166
effective search space: 582042972
effective search space used: 582042972
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)
