Alineamiento de B.Bigemina contra selenoproteínas de Drosophila
SLA/LP
TBLASTN 2.2.17 [Aug-26-2007]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= gi|45550694|ref|NP_649556.2| CG1427 CG1427-PA, isoform A
[Drosophila melanog
(448 letters)
Database: B_bigemina.contigs.fa
4396 sequences; 19,733,690 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Contig3744 33 0.31
Contig3897 33 0.33
Contig3683.0 32 0.60
Contig2932 32 0.79
Contig854 32 0.81
Contig1362 31 0.88
Contig846 31 0.97
>Contig3744
Length = 5928
Score = 32.7 bits (73), Expect = 0.31, Method: Composition-based stats.
Identities = 31/90 (34%), Positives = 39/90 (43%), Gaps = 9/90 (10%)
Frame = +3
Query: 113 LLARLTNALI--LDLIRGIGLPSC-----AGCFL--VPMCTGMTLTLCLQSLRKRRPGAR 163
L+ARLT L L L G LP C A C + + +C G +L LCL + PGAR
Sbjct: 2316 LVARLTLRLCASLALCLGARLPLCLVASLARCLVASLALCPGASLALCLVACLALWPGAR 2495
Query: 164 YVLWSRIDQKSCFKAITATGLVPVVIPCLI 193
L+ C A LV CL+
Sbjct: 2496 LALFLVARLARCLVARLTRCLVASSTRCLV 2585
>Contig3897
Length = 12828
Score = 32.7 bits (73), Expect = 0.33, Method: Composition-based stats.
Identities = 25/68 (36%), Positives = 29/68 (42%), Gaps = 7/68 (10%)
Frame = -2
Query: 123 LDLIRGIGLPSCAGCFL-------VPMCTGMTLTLCLQSLRKRRPGARYVLWSRIDQKSC 175
L L G LP C G L + +C G L LCL + R GAR L S +D
Sbjct: 5594 LALCLGARLPLCLGACLALCLVASLALCLGARLALCLGARSTRCLGARLALSSIVDSSLN 5415
Query: 176 FKAITATG 183
AI TG
Sbjct: 5414 LNAIQFTG 5391
>Contig3683.0
Length = 4137
Score = 32.0 bits (71), Expect = 0.60, Method: Composition-based stats.
Identities = 25/69 (36%), Positives = 31/69 (44%)
Frame = -3
Query: 131 LPSCAGCFLVPMCTGMTLTLCLQSLRKRRPGARYVLWSRIDQKSCFKAITATGLVPVVIP 190
LP C C +P C G TLTLCL GAR L C +A A LV +
Sbjct: 3607 LPLCL-CASLPRCLGATLTLCL--------GARSTLCLGASLPLCLRARLAPCLVARLAL 3455
Query: 191 CLIKGESLN 199
CL+ +L+
Sbjct: 3454 CLVASLALS 3428
>Contig2932
Length = 11681
Score = 31.6 bits (70), Expect = 0.79, Method: Composition-based stats.
Identities = 34/106 (32%), Positives = 42/106 (39%), Gaps = 5/106 (4%)
Frame = -1
Query: 132 PSCAGCFLVPMCTGMTLTLCLQSLRKRRPGARYVLWSRIDQKSCFKAITATGLVPVVIPC 191
P C G +C G TLT CL + GA L C A +A LV + C
Sbjct: 6803 PLCLGAS-SALCLGATLTRCLVASLALCLGASSALCLVARSPRCLSARSALSLVASLPLC 6627
Query: 192 LIKGESLNTNVDLFREKIKS----LGVDSILCL-YTTTSCFAPRNS 232
L +L L R + S LG S LCL T T C R++
Sbjct: 6626 LDASLALCLGATLTRCLVASLALCLGASSALCLGATLTLCLGARST 6489
>Contig854
Length = 2089
Score = 31.6 bits (70), Expect = 0.81, Method: Composition-based stats.
Identities = 31/84 (36%), Positives = 37/84 (44%), Gaps = 2/84 (2%)
Frame = -3
Query: 112 TLLARLTNALI--LDLIRGIGLPSCAGCFLVPMCTGMTLTLCLQSLRKRRPGARYVLWSR 169
+LLARLT L+ L L L C G L P+C G TLCL + GAR L
Sbjct: 782 SLLARLTRCLVASLALCLCARLALCLGASL-PLCLGAWSTLCLGASLALCLGARLALCLG 606
Query: 170 IDQKSCFKAITATGLVPVVIPCLI 193
C A A LV + CL+
Sbjct: 605 ASLPRCLVARLALCLVARLPRCLV 534
>Contig1362
Length = 9418
Score = 31.2 bits (69), Expect = 0.88, Method: Composition-based stats.
Identities = 21/67 (31%), Positives = 30/67 (44%)
Frame = -3
Query: 132 PSCAGCFLVPMCTGMTLTLCLQSLRKRRPGARYVLWSRIDQKSCFKAITATGLVPVVIPC 191
P+ C +P+C G +LTLCL + GAR L C A + LV + C
Sbjct: 6365 PNDGSCASLPLCLGASLTLCLGASLALCLGARLALCLGASLPLCLVACLSR*LVASLARC 6186
Query: 192 LIKGESL 198
L+ +L
Sbjct: 6185 LVASLAL 6165
>Contig846
Length = 1960
Score = 31.2 bits (69), Expect = 0.97, Method: Composition-based stats.
Identities = 23/56 (41%), Positives = 28/56 (50%), Gaps = 2/56 (3%)
Frame = -1
Query: 113 LLARLTNALILDLIRGIG--LPSCAGCFLVPMCTGMTLTLCLQSLRKRRPGARYVL 166
L+A L L+ L R +G LP C G L P C G +L LCL + R GA L
Sbjct: 934 LIASLPLCLVASLPRCLGACLPRCLGARL-PRCLGASLALCLSASLPRCLGASLAL 770
Database: B_bigemina.contigs.fa
Posted date: Feb 28, 2008 11:56 AM
Number of letters in database: 19,733,690
Number of sequences in database: 4396
Lambda K H
0.320 0.136 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4396
Number of Hits to DB: 10,559,300
Number of extensions: 167152
Number of successful extensions: 1271
Number of sequences better than 1.0: 30
Number of HSP's gapped: 1178
Number of HSP's successfully gapped: 31
Length of query: 448
Length of database: 6,577,896
Length adjustment: 100
Effective length of query: 348
Effective length of database: 6,138,296
Effective search space: 2136127008
Effective search space used: 2136127008
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 43 (21.2 bits)
SPS1
TBLASTN 2.2.17 [Aug-26-2007]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= gi|2411487|gb|AAB88790.1| selenophosphate synthetase
[Drosophila melanogaster]
(398 letters)
Database: B_bigemina.contigs.fa
4396 sequences; 19,733,690 total letters
Searching..................................................done
***** No hits found ******
Database: B_bigemina.contigs.fa
Posted date: Feb 28, 2008 11:56 AM
Number of letters in database: 19,733,690
Number of sequences in database: 4396
Lambda K H
0.321 0.136 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4396
Number of Hits to DB: 9,204,411
Number of extensions: 136460
Number of successful extensions: 669
Number of sequences better than 1.0: 0
Number of HSP's gapped: 669
Number of HSP's successfully gapped: 0
Length of query: 398
Length of database: 6,577,896
Length adjustment: 99
Effective length of query: 299
Effective length of database: 6,142,692
Effective search space: 1836664908
Effective search space used: 1836664908
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 43 (21.2 bits)
SPS2
TBLASTN 2.2.17 [Aug-26-2007]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= gi|28574119|ref|NP_477478.4| Selenophosphate synthetase
2[Drosophila melan]
(370 letters)
Database: B_bigemina.contigs.fa
4396 sequences; 19,733,690 total letters
Searching..................................................done
***** No hits found ******
Database: B_bigemina.contigs.fa
Posted date: Feb 28, 2008 11:56 AM
Number of letters in database: 19,733,690
Number of sequences in database: 4396
Lambda K H
0.320 0.136 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4396
Number of Hits to DB: 7,626,367
Number of extensions: 106203
Number of successful extensions: 392
Number of sequences better than 1.0: 0
Number of HSP's gapped: 392
Number of HSP's successfully gapped: 0
Length of query: 370
Length of database: 6,577,896
Length adjustment: 98
Effective length of query: 272
Effective length of database: 6,147,088
Effective search space: 1672007936
Effective search space used: 1672007936
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 43 (21.2 bits)
SelH
TBLASTN 2.2.17 [Aug-26-2007]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= gi|28571177|ref|NP_572903.3| BthD selenoprotein CG11177-PA
[Drosophila mr]
(249 letters)
Database: B_bigemina.contigs.fa
4396 sequences; 19,733,690 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Contig3887 31 0.48
>Contig3887
Length = 25891
Score = 31.2 bits (69), Expect = 0.48, Method: Composition-based stats.
Identities = 23/98 (23%), Positives = 44/98 (44%)
Frame = +2
Query: 149 KKSESTEEAQENEAPTSTSTSRKSKKEQKSEEEPTQVDSKEAKQSKELVKTKRQPKAQKK 208
+KS E ++A ++ + + E+ E +KEA++SKE K + Q
Sbjct: 14789 RKSAVIVEVAADDASNASQPAAEGTAEEAGERAANAEQAKEAQESKEHDKADSAKEEQAA 14968
Query: 209 QAKASESQEEVAEDKPPSSQKRKRTTRSSTDEATAGAK 246
+ ++ ES + + S + R + S D+A AG +
Sbjct: 14969 EPESKESSDAEQTTESGSEDQGNRENKKSDDKAEAGKE 15082
Database: B_bigemina.contigs.fa
Posted date: Mar 4, 2008 12:01 PM
Number of letters in database: 19,733,690
Number of sequences in database: 4396
Lambda K H
0.305 0.121 0.320
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4396
Number of Hits to DB: 4,625,062
Number of extensions: 54524
Number of successful extensions: 397
Number of sequences better than 1.0: 30
Number of HSP's gapped: 389
Number of HSP's successfully gapped: 47
Length of query: 249
Length of database: 6,577,896
Length adjustment: 94
Effective length of query: 155
Effective length of database: 6,164,672
Effective search space: 955524160
Effective search space used: 955524160
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 43 (21.9 bits)
S2: 41 (20.4 bits)
SelK
TBLASTN 2.2.17 [Aug-26-2007]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= gi|28571165|ref|NP_572763.3| Selenoprotein G CG1844-PA
[Drosophila melanogast
(110 letters)
Database: B_bigemina.contigs.fa
4396 sequences; 19,733,690 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Contig4132 28 1.00
>Contig4132
Length = 50512
Score = 27.7 bits (60), Expect = 1.00, Method: Composition-based stats.
Identities = 15/30 (50%), Positives = 16/30 (53%)
Frame = +1
Query: 59 GGGGGWGGGGGGGGGGGGGRPGSGSGGLRP 88
GGG G G G GG G PGSGS +P
Sbjct: 13156 GGGKGPASGAGQQGGTSKGSPGSGSKATQP 13245
Database: B_bigemina.contigs.fa
Posted date: Feb 28, 2008 11:56 AM
Number of letters in database: 19,733,690
Number of sequences in database: 4396
Lambda K H
0.322 0.149 0.534
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4396
Number of Hits to DB: 3,676,195
Number of extensions: 75439
Number of successful extensions: 8125
Number of sequences better than 1.0: 209
Number of HSP's gapped: 4572
Number of HSP's successfully gapped: 967
Length of query: 110
Length of database: 6,577,896
Length adjustment: 82
Effective length of query: 28
Effective length of database: 6,217,424
Effective search space: 174087872
Effective search space used: 174087872
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 38 (19.2 bits)
SBP2
TBLASTN 2.2.17 [Aug-26-2007]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= gi|21355989|ref|NP_648204.1| CG7066 CG7066-PA [Drosophila
melanogaster]
(313 letters)
Database: B_bigemina.contigs.fa
4396 sequences; 19,733,690 total letters
Searching..................................................done
***** No hits found ******
Database: B_bigemina.contigs.fa
Posted date: Feb 28, 2008 11:56 AM
Number of letters in database: 19,733,690
Number of sequences in database: 4396
Lambda K H
0.319 0.132 0.377
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4396
Number of Hits to DB: 6,676,898
Number of extensions: 104494
Number of successful extensions: 561
Number of sequences better than 1.0: 2
Number of HSP's gapped: 560
Number of HSP's successfully gapped: 2
Length of query: 313
Length of database: 6,577,896
Length adjustment: 96
Effective length of query: 217
Effective length of database: 6,155,880
Effective search space: 1335825960
Effective search space used: 1335825960
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 42 (20.8 bits)
Secp43
TBLASTN 2.2.17 [Aug-26-2007]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= gi|28574707|ref|NP_608837.2| CG15440 CG15440-PA [Drosophila
melanogaster]
(336 letters)
Database: B_bigemina.contigs.fa
4396 sequences; 19,733,690 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Contig4184.3 67 2e-11
Contig3945 48 5e-06
Contig3934 48 5e-06
Contig4104.1 38 0.005
Contig3713 38 0.007
Contig4166.0 34 0.11
Contig4158 32 0.57
>Contig4184.3
Length = 137674
Score = 66.6 bits (161), Expect = 2e-11, Method: Composition-based stats.
Identities = 49/158 (31%), Positives = 78/158 (49%)
Frame = -1
Query: 8 LWMGSLESYMTENFIIAAFRKMGEDPTTVRLMRNKYTGEPAGYCFVNFISDDHALDAMHK 67
L++G L +TE + F +G ++R+ R+ T + GY +VN+ + A A+
Sbjct: 30733 LYVGDLLPEVTEAMLYEVFNGVGP-VASIRVCRDSVTRKSLGYAYVNYYNVQDAETALEC 30557
Query: 68 LNGKPIPGTNPIVRFRLNSASNSYKLPGNEREFSVWVGDLSSDVDDYQLYKVFSSKFTSI 127
LN I G + + S GN ++V +L +D LY FS F +I
Sbjct: 30556 LNYIDIKGHPARIMWSDRDPSLRKSGTGN-----IFVKNLDKAIDTKALYDTFS-HFGTI 30395
Query: 128 KTAKVILDSLGFSKGYGFVRFGIEDEQKSALYDMNGYI 165
+ KV +D+LG SKGYGFV + E+ K A+ +NG +
Sbjct: 30394 LSCKVAMDALGNSKGYGFVHYTTEESAKEAIEKVNGML 30281
Score = 37.0 bits (84), Expect = 0.012, Method: Composition-based stats.
Identities = 31/118 (26%), Positives = 54/118 (45%), Gaps = 1/118 (0%)
Frame = +2
Query: 49 GYCFVNFISDDHALDAMHKLNGKPIPGTNPIVRFRLNSASNSYKLPGNEREFSVWVGDLS 108
GYCF+ F + + A D KL+G G +R + + S + + V+V ++
Sbjct: 104231 GYCFLEFSTAELA-DMCFKLDGINYKGQTLKIRRPIEYGTTS-----SSDDTKVFVQNIP 104392
Query: 109 SDVDDYQLYKVFSSKFTSIKTAKVILD-SLGFSKGYGFVRFGIEDEQKSALYDMNGYI 165
+ D + K K + +A ++ D G +KGYGF F K A+ +NG++
Sbjct: 104393 PTMSDADI-KALLEKHGKLTSANLVKDLKTGQNKGYGFFEFDDSRAAKMAVCHLNGHV 104563
>Contig3945
Length = 13847
Score = 48.1 bits (113), Expect = 5e-06, Method: Composition-based stats.
Identities = 41/128 (32%), Positives = 66/128 (51%), Gaps = 6/128 (4%)
Frame = +3
Query: 53 VNFISDDHALDAMHKLNGKPIPGTNPIVRFRLNSASNS-YKLPG----NEREFSVWVGDL 107
+N I+D+ A ++ + GT R L A+ S + P E +F ++ GDL
Sbjct: 13401 INRITDEEAA----RMGSRMAVGTKGNGRIHLRKAAGSVWNDPTLDDWPENDFRIFCGDL 13568
Query: 108 SSDVDDYQLYKVFSSKFTSIKTAKVILD-SLGFSKGYGFVRFGIEDEQKSALYDMNGYIG 166
+DV D L F K+ + + A+VI D + G SKGYGFV ++ +AL +MN
Sbjct: 13569 GNDVTDDVLANAFK-KYPTFQRARVIRDRNSGKSKGYGFVSMMSAEDMLAALNEMNNKF- 13742
Query: 167 LGTKPIKI 174
+G +PI++
Sbjct: 13743 VGNRPIRV 13766
>Contig3934
Length = 22845
Score = 48.1 bits (113), Expect = 5e-06, Method: Composition-based stats.
Identities = 30/79 (37%), Positives = 47/79 (59%), Gaps = 1/79 (1%)
Frame = -3
Query: 97 EREFSVWVGDLSSDVDDYQLYKVFSSKFTSIKTAKVILD-SLGFSKGYGFVRFGIEDEQK 155
E +F ++ GDL +DV D L F K+ + + A+VI D + G SKGYGFV ++
Sbjct: 21145 ENDFRIFCGDLGNDVTDDVLANAFK-KYPTFQRARVIRDRNSGKSKGYGFVSMMSAEDML 20969
Query: 156 SALYDMNGYIGLGTKPIKI 174
+AL +MN +G +PI++
Sbjct: 20968 AALNEMNNKF-VGNRPIRV 20915
>Contig4104.1
Length = 37990
Score = 38.1 bits (87), Expect = 0.005, Method: Composition-based stats.
Identities = 29/101 (28%), Positives = 55/101 (54%), Gaps = 3/101 (2%)
Frame = -3
Query: 98 REFSVWVGDLSSDVDDYQLYKVFSSKFTSIKTAKVILDSL-GFSKGYGFVRFGIEDEQKS 156
+E +++VG++ + VD+ L++ F + +K + D + G +GY FV F ED+
Sbjct: 8885 QEATLYVGNVDTQVDEELLWEFFV-QVGPVKHVHIPRDKVTGHHQGYAFVEFESEDDADY 8709
Query: 157 ALYDMNGYIGLGTKPIKICNAVPKPKS--ELGGAVGEGNTN 195
A+ +N ++ L KP++ CN + K E+G + GN +
Sbjct: 8708 AIRILN-FVKLYNKPLR-CNKASRDKQNYEIGANLFIGNLD 8592
>Contig3713
Length = 2421
Score = 37.7 bits (86), Expect = 0.007, Method: Composition-based stats.
Identities = 26/86 (30%), Positives = 43/86 (50%), Gaps = 11/86 (12%)
Frame = -2
Query: 8 LWMGSLESYMTENFIIAAFRKMGEDPTT-VRLMRNKYTGEPAGYCFVNFISDDHALDAMH 66
L++G++++ + E + F ++G P V + R+K TG GY FV F S+DHA +
Sbjct: 593 LYVGNVDTQVDEELLWEFFVQVG--PVKHVHIPRDKVTGHHQGYAFVEFESEDHADYPLR 420
Query: 67 KLN----------GKPIPGTNPIVRF 82
LN G +P T+ +F
Sbjct: 419 FLNFLKRVKKPFRGNKVPRTSQTSKF 342
>Contig4166.0
Length = 90045
Score = 33.9 bits (76), Expect = 0.11, Method: Composition-based stats.
Identities = 21/88 (23%), Positives = 44/88 (50%), Gaps = 1/88 (1%)
Frame = -1
Query: 84 LNSASNSYKLPGNEREFSVWVGDLSSDVDDYQLYKVFSSKFTSIKTAKVILD-SLGFSKG 142
++ S + + P ++++ + ++ Y L + FS +F I ++++ D S G KG
Sbjct: 30189 FDNPSGASQAPAGPPGANLFIFHIPNEWTQYDLVRTFS-QFGKILSSRIASDKSTGRHKG 30013
Query: 143 YGFVRFGIEDEQKSALYDMNGYIGLGTK 170
Y FV + D A+ +NG+ +G +
Sbjct: 30012 YAFVSYDSPDSAAQAIQHLNGFTVMGKR 29929
Score = 32.7 bits (73), Expect = 0.25, Method: Composition-based stats.
Identities = 19/58 (32%), Positives = 32/58 (55%)
Frame = -1
Query: 18 TENFIIAAFRKMGEDPTTVRLMRNKYTGEPAGYCFVNFISDDHALDAMHKLNGKPIPG 75
T+ ++ F + G+ ++ R+ +K TG GY FV++ S D A A+ LNG + G
Sbjct: 30105 TQYDLVRTFSQFGKILSS-RIASDKSTGRHKGYAFVSYDSPDSAAQAIQHLNGFTVMG 29935
>Contig4158
Length = 87318
Score = 31.6 bits (70), Expect = 0.57, Method: Composition-based stats.
Identities = 19/64 (29%), Positives = 36/64 (56%)
Frame = +3
Query: 101 SVWVGDLSSDVDDYQLYKVFSSKFTSIKTAKVILDSLGFSKGYGFVRFGIEDEQKSALYD 160
+++V L ++ DD F +F ++ +A+V DS G +KG+GF+ + +A+
Sbjct: 67644 NLFVFHLPAEWDDADFVLNFQ-QFGTVLSARVQRDSAGRNKGFGFISYDNPLSALNAIKS 67820
Query: 161 MNGY 164
MNG+
Sbjct: 67821 MNGF 67832
Database: B_bigemina.contigs.fa
Posted date: Feb 28, 2008 11:56 AM
Number of letters in database: 19,733,690
Number of sequences in database: 4396
Lambda K H
0.314 0.131 0.390
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4396
Number of Hits to DB: 7,395,256
Number of extensions: 101067
Number of successful extensions: 451
Number of sequences better than 1.0: 9
Number of HSP's gapped: 447
Number of HSP's successfully gapped: 11
Length of query: 336
Length of database: 6,577,896
Length adjustment: 97
Effective length of query: 239
Effective length of database: 6,151,484
Effective search space: 1470204676
Effective search space used: 1470204676
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 42 (20.8 bits)
PSTK
TBLASTN 2.2.17 [Aug-26-2007]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= gi|19920352|ref|NP_608329.1| CG12788 CG12788-PA [Drosophila
melanogaster]
(292 letters)
Database: B_bigemina.contigs.fa
4396 sequences; 19,733,690 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Contig4120 31 0.70
>Contig4120
Length = 42572
Score = 30.8 bits (68), Expect = 0.70, Method: Composition-based stats.
Identities = 23/70 (32%), Positives = 35/70 (50%)
Frame = +2
Query: 130 MASSLDSCLQANSLRSDATRVPVDVVRQMNERLEVPDTSEAWERNSLTLNGLDMDTTGSA 189
+ S+LDS +++ + D+V+ RLE P A++R S L+G+D D T A
Sbjct: 31088 LQSALDSSNLPSTIVLLEGKSDGDIVKAAMRRLEYP-LQVAFKRLSFALSGVDADDTSRA 31264
Query: 190 LLAFIASLLD 199
L LLD
Sbjct: 31265 LTQRSRDLLD 31294
Database: B_bigemina.contigs.fa
Posted date: Mar 4, 2008 11:40 AM
Number of letters in database: 19,733,690
Number of sequences in database: 4396
Lambda K H
0.322 0.138 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4396
Number of Hits to DB: 6,722,194
Number of extensions: 111013
Number of successful extensions: 613
Number of sequences better than 1.0: 1
Number of HSP's gapped: 613
Number of HSP's successfully gapped: 1
Length of query: 292
Length of database: 6,577,896
Length adjustment: 96
Effective length of query: 196
Effective length of database: 6,155,880
Effective search space: 1206552480
Effective search space used: 1206552480
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 42 (20.8 bits)
tRNASec
BLASTN 2.2.17 [Aug-26-2007]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Predicted RNA/mRNA Sequence (introns spliced out) Hide
(90 letters)
Database: B_bigemina.contigs.fa
4396 sequences; 19,733,690 total letters
Searching..................................................done
***** No hits found ******
Database: B_bigemina.contigs.fa
Posted date: Feb 28, 2008 11:56 AM
Number of letters in database: 19,733,690
Number of sequences in database: 4396
Lambda K H
1.37 0.711 1.31
Gapped
Lambda K H
1.37 0.711 1.31
Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Sequences: 4396
Number of Hits to DB: 13,786
Number of extensions: 591
Number of successful extensions: 171
Number of sequences better than 1.0: 0
Number of HSP's gapped: 171
Number of HSP's successfully gapped: 0
Length of query: 90
Length of database: 19,733,690
Length adjustment: 15
Effective length of query: 75
Effective length of database: 19,667,750
Effective search space: 1475081250
Effective search space used: 1475081250
X1: 11 (21.8 bits)
X2: 15 (29.7 bits)
X3: 25 (49.6 bits)
S1: 12 (24.3 bits)
S2: 16 (32.2 bits)