TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= SPP00000030_1.0 # Protein # Selenoprotein W1 (SelW1) # Hs (87 letters) Database: genome.fa 4921 sequences; 228,543,505 total letters Searching..................................................done ***** No hits found ****** TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= SPP00000031_1.0 # Protein # Selenoprotein W2 (SelW2) # Hs (115 letters) Database: genome.fa 4921 sequences; 228,543,505 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value supercont1.5 of Phytophthora infestans 30 2.4 >supercont1.5 of Phytophthora infestans Length = 4768765 Score = 29.6 bits (65), Expect = 2.4, Method: Composition-based stats. Identities = 14/31 (45%), Positives = 21/31 (67%), Gaps = 2/31 (6%) Frame = +2 Query: 83 FPYEKDLIEAIRRAS--NGETLEKITNSRPP 111 FPY++D EAI A+ N E+L+ TN++ P Sbjct: 3826637 FPYQQDTFEAIAVAAL*NIESLKMYTNTKEP 3826729 Database: genome.fa Posted date: Feb 28, 2008 11:00 AM Number of letters in database: 228,543,505 Number of sequences in database: 4921 Lambda K H 0.322 0.141 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4921 Number of Hits to DB: 40,459,178 Number of extensions: 380262 Number of successful extensions: 1570 Number of sequences better than 10.0: 1 Number of HSP's gapped: 1570 Number of HSP's successfully gapped: 1 Length of database: 76,181,168 Neighboring words threshold: 13 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 46 (22.3 bits)