TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= SPP00000013_1.0 # Protein # Selenoprotein 15 (Sel15) # Hs (124 letters) Database: genome.fa 4921 sequences; 228,543,505 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value supercont1.75 of Phytophthora infestans 31 0.85 supercont1.3 of Phytophthora infestans 31 0.85 supercont1.169 of Phytophthora infestans 23 5.8 >supercont1.75 of Phytophthora infestans Length = 515009 Score = 31.2 bits (69), Expect = 0.85, Method: Composition-based stats. Identities = 11/22 (50%), Positives = 14/22 (63%) Frame = +2 Query: 102 PQVQVCPWFRPCIKAFGRQWEH 123 P Q+ PWFRPCI+ G + H Sbjct: 29915 PNRQLSPWFRPCIRPVGLRPSH 29980 >supercont1.3 of Phytophthora infestans Length = 5458067 Score = 31.2 bits (69), Expect = 0.85, Method: Composition-based stats. Identities = 11/22 (50%), Positives = 14/22 (63%) Frame = -1 Query: 102 PQVQVCPWFRPCIKAFGRQWEH 123 P Q+ PWFRPCI+ G + H Sbjct: 835418 PNRQLSPWFRPCIRPVGLRPSH 835353 >supercont1.169 of Phytophthora infestans Length = 178907 Score = 23.5 bits (49), Expect(2) = 5.8, Method: Composition-based stats. Identities = 9/23 (39%), Positives = 13/23 (56%) Frame = -2 Query: 101 FPQVQVCPWFRPCIKAFGRQWEH 123 F V+V PW R C+ R ++H Sbjct: 171073 FSPVRVLPWLRTCVLFRSRAFQH 171005 Score = 22.7 bits (47), Expect(2) = 5.8, Method: Composition-based stats. Identities = 8/19 (42%), Positives = 10/19 (52%) Frame = -3 Query: 91 LEVCGUKLGRFPQVQVCPW 109 L CG + GR + V PW Sbjct: 171156 LAYCGLRFGRLTVLHVYPW 171100 Database: genome.fa Posted date: Feb 28, 2008 11:00 AM Number of letters in database: 228,543,505 Number of sequences in database: 4921 Lambda K H 0.330 0.142 0.482 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4921 Number of Hits to DB: 40,564,436 Number of extensions: 505376 Number of successful extensions: 2655 Number of sequences better than 10.0: 16 Number of HSP's gapped: 2655 Number of HSP's successfully gapped: 27 Length of query: 124 Length of database: 76,181,168 Length adjustment: 99 Effective length of query: 25 Effective length of database: 75,693,989 Effective search space: 1892349725 Effective search space used: 1892349725 Neighboring words threshold: 13 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 47 (22.7 bits)