TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= SPP00000037_1.0 # Prot # SECIS binding protein 2 (SBP2) # Hs (854 letters) Database: genome.fa 4921 sequences; 228,543,505 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value supercont1.10 of Phytophthora infestans 46 7e-04 >supercont1.10 of Phytophthora infestans Length = 3761764 Score = 46.2 bits (108), Expect = 7e-04, Method: Composition-based stats. Identities = 36/132 (27%), Positives = 61/132 (46%), Gaps = 24/132 (18%) Frame = -1 Query: 657 DRMYQKDPVKAKTKRRLVLGLREVXXXXXXXXXXCVIISPN---CEKIQSKGGLDDTLHT 713 +R Y ++ K RRLVLG+ EV +II+ + CE ++ K Sbjct: 1276657 ERAYAQNKA-VKRSRRLVLGMHEVRRGLLCNKIVLLIIATDLDGCEAVEEK------YRE 1276499 Query: 714 IIDYACEQNIP---------FVFA------------LNRKALGRSLNKAVPVSVVGIFSY 752 ++ A E +P F++ L R+ LG+ L+K+V VS VG++S Sbjct: 1276498 LVAIAKEHKVPIHAPVNRCGFLYEDALYGWPNAKSFLARRKLGKMLHKSVRVSCVGVYSI 1276319 Query: 753 DGAQDQFHKMVE 764 +GA D F ++++ Sbjct: 1276318 EGANDLFQQILQ 1276283 Database: genome.fa Posted date: Feb 28, 2008 11:00 AM Number of letters in database: 228,543,505 Number of sequences in database: 4921 Lambda K H 0.311 0.128 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4921 Number of Hits to DB: 196,823,210 Number of extensions: 2759287 Number of successful extensions: 14296 Number of sequences better than 10.0: 7 Number of HSP's gapped: 14292 Number of HSP's successfully gapped: 7 Length of query: 854 Length of database: 76,181,168 Length adjustment: 123 Effective length of query: 731 Effective length of database: 75,575,885 Effective search space: 55245971935 Effective search space used: 55245971935 Neighboring words threshold: 13 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 54 (25.4 bits)