TBLASTN 2.2.17 [Aug-26-2007]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= SPP00000001_1.0 # Prot # Iodothyronine deiodinase 1 (DI1) # Hs
         (249 letters)

Database: genome.fa 
           4921 sequences; 228,543,505 total letters

Searching..................................................done

 ***** No hits found ******


TBLASTN 2.2.17 [Aug-26-2007]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= SPP00000002_1.0 # Prot # Iodothyronine deiodinase 2 (DI2) # Hs
         (265 letters)

Database: genome.fa 
           4921 sequences; 228,543,505 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

supercont1.13 of Phytophthora infestans                                33   0.98 
supercont1.84 of Phytophthora infestans                                33   1.4  

>supercont1.13 of Phytophthora infestans
          Length = 3221495

 Score = 33.5 bits (75), Expect = 0.98,   Method: Composition-based stats.
 Identities = 22/52 (42%), Positives = 29/52 (55%), Gaps = 2/52 (3%)
 Frame = -3

Query: 8     LLITLQILPVFFSNCLFLALYDSV--ILLKHVVLLLSRSKSTRGEWRRMLTS 57
             ++  LQIL V     L L   D V  I+L +V  ++  +KSTRG WRR L S
Sbjct: 94785 MVAPLQILAVQHRAVLELYSQDGVFPIVLLYVNAIMCATKSTRGSWRRCLIS 94630


>supercont1.84 of Phytophthora infestans
          Length = 492161

 Score = 33.1 bits (74), Expect = 1.4,   Method: Composition-based stats.
 Identities = 19/57 (33%), Positives = 26/57 (45%)
 Frame = -2

Query: 177    SLSFEVKKHQNQEDRCAAAQQLLERFSLPPQCRVVADRMDNNANIAYGVAFERVCIV 233
              +L  E  + Q    RC     L+E+ S      VV   +  +ANI   V FER C+V
Sbjct: 258862 TLDHEASRRQRNASRCDIFDALIEKHS------VVKKYLAADANIVKDVDFERACVV 258710


TBLASTN 2.2.17 [Aug-26-2007]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= SPP00000003_1.0 # Prot # Iodothyronine deiodinase 3 (DI3) # Hs
         (278 letters)

Database: genome.fa 
           4921 sequences; 228,543,505 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

supercont1.40 of Phytophthora infestans                                36   0.22 

>supercont1.40 of Phytophthora infestans
          Length = 1445775

 Score = 35.8 bits (81), Expect = 0.22,   Method: Composition-based stats.
 Identities = 24/83 (28%), Positives = 38/83 (45%), Gaps = 1/83 (1%)
 Frame = -3

Query: 181    WVTTDSPYIIPQHRSLEDRVSAARVLQQGAPGCALVLD-TMANSSSSAYGAYFERLYVIQ 239
              W T  SPY+IP   ++   +    +   G P C L+++  MA++ S A       L+  Q
Sbjct: 636724 WATAFSPYVIPMAGTMGRTMVIPGIRDSGFPSCLLIMNHGMASADSEARKM---ALFTNQ 636554

Query: 240    SGTIMYQGGRGPDGYQVSELRTW 262
               G   Y+ G     + V+  RTW
Sbjct: 636553 QGVWNYESGT----WDVAPGRTW 636497


  Database: genome.fa
    Posted date:  Feb 28, 2008 11:00 AM
  Number of letters in database: 228,543,505
  Number of sequences in database:  4921
  
Lambda     K      H
   0.325    0.139    0.450 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4921
Number of Hits to DB: 241,061,352
Number of extensions: 4020485
Number of successful extensions: 17015
Number of sequences better than 10.0: 6
Number of HSP's gapped: 17015
Number of HSP's successfully gapped: 6
Length of database: 76,181,168
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 50 (23.9 bits)