TBLASTN 2.2.17 [Aug-26-2007]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= SPP00000033_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Homo sapiens # Complete
         (448 letters)

Database: genome.fa 
           2809 sequences; 26,957,458 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Plasmodium_vivax_SaI-1|ctg_7052|2005-09-01|ds-DNA|Plasmodium_viv...    44   2e-04
Plasmodium_vivax_SaI-1|ctg_7179|2005-09-01|ds-DNA|Plasmodium_viv...    29   7.3  

>Plasmodium_vivax_SaI-1|ctg_7052|2005-09-01|ds-DNA|Plasmodium_vivax_TIGR
          Length = 1198945

 Score = 43.9 bits (102), Expect = 2e-04,   Method: Composition-based stats.
 Identities = 25/84 (29%), Positives = 49/84 (58%)
 Frame = -1

Query: 132    SLVQTTDFFYPLVEDPYMMGRIACANVLSDLYAMGITECDNMLMLLSVSQSMSEEEREKV 191
              +LVQT DFF   ++D Y++G I   + LSD+Y+MG T    + +L+ V  ++ ++ ++++
Sbjct: 402214 ALVQTIDFFKSFIDDEYILGEIIAIHCLSDVYSMGGTGICALCVLI-VKDNIEKKLQQRL 402038

Query: 192    TPLMVKGFRDAAEEGGTAVTGGQT 215
                ++    +   EE    ++GG T
Sbjct: 402037 QNILTGCCQKLKEE-KCVLSGGHT 401969



 Score = 42.7 bits (99), Expect = 5e-04,   Method: Composition-based stats.
 Identities = 30/100 (30%), Positives = 41/100 (41%), Gaps = 1/100 (1%)
 Frame = -1

Query: 241    SAVVGDVLVLTKPLGTQVAVNAHQWLDNPERWNKVKMVVSREEVELAYQEAMFNMATLNR 300
              S   GD+++ TK  G    + AH       RW                   +  M   NR
Sbjct: 401635 SIKAGDIIITTKMFGFGFIMAAHICKKAKARW---------------IYNCLDEMLLSNR 401501

Query: 301    TAA-GLMHTFNAHAATDITGFGILGHSQNLAKQQRNEVSF 339
               +   L+   NA A TD+TGFG+LGH   + K  R E+ F
Sbjct: 401500 KSGLYLLQNNNAKACTDVTGFGVLGHLNEMIKCSRREIYF 401381



 Score = 31.6 bits (70), Expect = 1.2,   Method: Composition-based stats.
 Identities = 21/70 (30%), Positives = 35/70 (50%), Gaps = 5/70 (7%)
 Frame = +1

Query: 145     EDPYMMGRIACANVL-----SDLYAMGITECDNMLMLLSVSQSMSEEEREKVTPLMVKGF 199
               +D  +MG I C   +       LY+  +    N ++LLS  + +S+EER+  +P      
Sbjct: 1032415 DDGPLMGSILCFVSMPVYFKKQLYSCQVVIYRNQIILLSPKECLSDEERKYFSPW----- 1032579

Query: 200     RDAAEEGGTA 209
                 A +EGG+A
Sbjct: 1032580 -SALKEGGSA 1032606


>Plasmodium_vivax_SaI-1|ctg_7179|2005-09-01|ds-DNA|Plasmodium_vivax_TIGR
          Length = 1986252

 Score = 28.9 bits (63), Expect = 7.3,   Method: Composition-based stats.
 Identities = 19/69 (27%), Positives = 34/69 (49%)
 Frame = -1

Query: 145    EDPYMMGRIACANVLSDLYAMGITECDNMLMLLSVSQSMSEEEREKVTPLMVKGFRDAAE 204
              E P M+ R   + ++  +Y+    E D        ++S S   ++ V    ++GF ++  
Sbjct: 881760 ETPSMLVRKEASELIKLIYSYDAAESD--------AKSASNSNQDVVKNNRIQGFGNSHF 881605

Query: 205    EGGTAVTGG 213
              E GTAV+GG
Sbjct: 881604 ERGTAVSGG 881578


  Database: genome.fa
    Posted date:  Feb 25, 2008  2:49 PM
  Number of letters in database: 26,957,458
  Number of sequences in database:  2809
  
Lambda     K      H
   0.317    0.133    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 2809
Number of Hits to DB: 13,328,902
Number of extensions: 214091
Number of successful extensions: 998
Number of sequences better than 10.0: 5
Number of HSP's gapped: 997
Number of HSP's successfully gapped: 8
Length of query: 448
Length of database: 8,985,819
Length adjustment: 102
Effective length of query: 346
Effective length of database: 8,699,301
Effective search space: 3009958146
Effective search space used: 3009958146
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 46 (22.3 bits)
# TBLASTN 2.2.17 [Aug-26-2007]
# Query: SPP00000033_1.0 # Protein # Selenophosphate synthetase 2 (SPS2) # Homo sapiens # Complete
# Database: genome.fa
# Fields: Query id, Subject id, % identity, alignment length, mismatches, gap openings, q. start, q. end, s. start, s. end, e-value, bit score
SPP00000033_1.0	Plasmodium_vivax_SaI-1|ctg_7052|2005-09-01|ds-DNA|Plasmodium_vivax_TIGR	29.76	84	59	2	132	215	402214	401969	2e-04	43.9
SPP00000033_1.0	Plasmodium_vivax_SaI-1|ctg_7052|2005-09-01|ds-DNA|Plasmodium_vivax_TIGR	30.00	100	69	2	241	339	401635	401381	5e-04	42.7
SPP00000033_1.0	Plasmodium_vivax_SaI-1|ctg_7052|2005-09-01|ds-DNA|Plasmodium_vivax_TIGR	30.00	70	44	2	145	209	1032415	1032606	1.2	31.6
SPP00000033_1.0	Plasmodium_vivax_SaI-1|ctg_7179|2005-09-01|ds-DNA|Plasmodium_vivax_TIGR	27.54	69	50	1	145	213	881760	881578	7.3	28.9