CLUSTAL W (1.82) multiple sequence alignment
GPx4 ---------------------MS-------LGRLCRLLKPALLCGALAAPGLAGTMCA-- 30
Apis ---------------------MRSRPTDGILNSRTKLDKELIFLTVLFFCGVIGENCE-- 37
Aedes ---------------------MFLLR---LLTRPASSASNRTAAFGLFGTAMVAINLL-- 34
D.erecta+3 ------------------------------------------------------------
D.ananaessis+3 --------------------------------------LTQLLCLSAELKVVASACLSFV 22
D.pseudoobscura-3 ------------------------------------------------------------
D.virilis+2 -------------------------------------NPVRLLLLLVAFGLTASKFCCCL 23
D.simulans+2 ------------------------------------------------------------
Drosophila --------------------------------------MAGRSIVHFFLGSVAIALG--- 19
D.Mojavensis-2 ---------------------------------------FCLRRFIVACRWSSAKFCCFA 21
D.ananaessis+1 ------------------------------------------------------------
Glossina -----------------------------------------MMSFVIVNILIGLFFAG-- 17
Anopheles --------------------------------------MFSTKSVLLFVVGVLIAYGVNR 22
D.yakuba-1 --------QSLHAQLASRIDFHLRHFPSFTSASQIQLVRQTMFDKETIFPGLMAAVAL-- 50
D.erecta+1 ----------------------------------------TMFDKDTIFRGLMAAVAL-- 18
D.simulans-2 ----------------------------------------TMFDKETLFPGLLAAVAL-- 18
D.ananaessis-1 -------------------------------------LDAAMANTETVFLALTAGMGI-- 21
D.pseudoobscura-2 IRLSAVSLRTISPNAARRIDSPWRHLLEWSDGPSSATVKPKMPSKEVVFYGLMAAIAC-- 58
Ixodes -------------------------------------MALKRKPGCFSFRYIQAMWPLK- 22
GPx4 -------------------SRD--------------DWRCARSMHEFSAKDIDGHMVNLD 57
Apis -------------------DNNKKEECALAPLDQDKNWKSASTIYDFHAKDIHGNDVSLN 78
Aedes -------------------STSSVLACPSSQSKKTASGNEPSSVYDFSAVDIDGNKVDFE 75
D.erecta+3 -----------------------------------GDYKNAASIYEFTVKDTHGNDISLE 25
D.ananaessis+3 LCDRYMILKTCFA-ESNTQFQTHVSHPFQIDMS-NGDYKNAASIYEFTVKDTHGNDVSLD 80
D.pseudoobscura-3 --------------------SAMIFPLFQIDMSANGDYKNAASIYEFTVKDTHGSEVSLD 40
D.virilis+2 CSFLFFI-------------NCNAFYLLQLDMS-NGDYKNAASIYEFTVKDTHGNDVSLD 69
D.simulans+2 ----------------------------QIDMSANGDYKNAASIYEFTVKDTHGNDISLE 32
Drosophila ---------------------SYIYFTMQIDMSANGDYKNAASIYEFTVKDTHGNDVSLE 58
D.Mojavensis-2 YYSAFSR-----------SIIKPFRTLFKVDMS-DGDYKNAASIYEFNVKDTHGNDVSLE 69
D.ananaessis+1 ----------------------------PVAVA-LGSY-----IY-----------FTMQ 15
Glossina ---------------------LGTYYFYSKQQS-TTXSEEASSIYDFTVKDTYGNDVSLE 55
Anopheles --------------------FYSQSTTTQTDGT-MEDYKNAKSVYDFTVKDSQGADVSLE 61
D.yakuba-1 --------------------VAVLQTRSRLQQD-LQNMRWRLTIHALTVRDTFGRPVQLD 89
D.erecta+1 --------------------VTVLQTRSRLQQD-LQDMRWRLTIQALTVRDTFGKPVQLD 57
D.simulans-2 --------------------VVVLQTRSRLQQD-LQDMRWRLTIHALTVRDTFGNPVQLD 57
D.ananaessis-1 --------------------LVTLVTHYRLYQD-LETMRWRLKVLSLTVKDTFGQPISLN 60
D.pseudoobscura-2 --------------------VTTIVTHHRLAVQ-LFEMQWAQSVHSHTVLDTFHQRVDLR 97
Ixodes -------------------IAVLLLVAGLTLANSDEGWKNAKSIYEFSALDIDGNKVDFN 63
: . :
GPx4 KYRGFVCIVTNVASQUGKTEVNYTQLVDLHARYAEC-GLRILAFPCNQFGKQEPGSN-EE 115
Apis KYRGHVCIIVNVASNCGLTDTNYRELVQLYEKYNEKEGLRILAFPSNEFGGQEPGTS-VE 137
Aedes RYRGHVLIIVNVASKCGYTAGHYKELNELYEEYGETEGLRILAFPCNQFGNQEPGTN-EE 134
D.erecta+3 KYKGKVVLVVNIASKCGLTKNNYQKLTDLKEKYGER-GLVILNFPCNQFGSQMPEADGEA 84
D.ananaessis+3 KYKGQVVLVVNIASKCGLTKNNYQKLTDLKEKYGDK-GLVILNFPCNQFGSQMPEADGEA 139
D.pseudoobscura-3 KYKGRVLLVVNIASKCGLTKNNYQKLTELKEKFGER-GLTILNFPCNQFNSQMPEADGEA 99
D.virilis+2 KYKGRVVLIVNIASKCGLTKNNYQKLTDLKEKYGER-GLTILNFPCNQFNSQMPEADGEA 128
D.simulans+2 KYKGKVVLVVNIASKCGLTKNNYQKLTDLKEKYGER-GLVILNFPCNQFGSQMPEADGEA 91
Drosophila KYKGKVVLVVNIASKCGLTKNNYEKLTDLKEKYGER-GLVILNFPCNQFGSQMPEADGEA 117
D.Mojavensis-2 KYKGQVILIVNIASKCGLTKNNYKKLTDLKEKYGER-GTDHPELPMQSI--WLPNARGRW 126
D.ananaessis+1 IDKGQVFLVVNIASQCGLTKNNYQTLTDLKEKYGDI-G---------------------- 52
Glossina QYRGHVVLIVNIASQCGLTKNNYKKLTDLREKYGDK-GLKILNFPCNQFGSQMPESDGEP 114
Anopheles KYRGKVLLIVNIASQCGLTKGNYAELTELSQKYADK-DFKILSFPCNQFGGQMPEGDGEE 120
D.yakuba-1 KFAGQVMLIVNIASKCGLTSTQYNGLRYLLEEYEDR-GLRILNFPCNQFGAQMPESDGQE 148
D.erecta+1 KFAGHVMLIVNIASKCGLTSSQYNGLRYLLEEYEDR-GLRILNFPCNQFGGQMPESDGQE 116
D.simulans-2 IFAGHVMLIVNIASRCGLTLSQYNGLRYLLEEYEEQ-GLRILNFPCNQFGGQMPESDGQE 116
D.ananaessis-1 QFAGQVLLVVNIASRCGLTSSQYAGLHELREKYEER-GLSILNFPCNQFGAQMPESDGRE 119
D.pseudoobscura-2 YFAGHVLVIVNIASQCGLTGQQYAGLTQLLAKYGER-GLRIN-FPCNQFGQQMPQSDGQE 155
Ixodes KYRGHVTQIVNVACKCLLTQEHYKKLSALYHKYSESKGLRIMAFPTNDFAKQEPWAE-PE 122
* * :.*:*.. * :* * * .: : .
GPx4 IKEFAAGYN-VKFDMFSKICVN----------------------GDDAHPLWKWMKIQPK 152
Apis ILEFVKKYN-VTFDLFEKINVN----------------------GDNAHPLWKWLKTQAN 174
Aedes IKHFARVEKGAKFDLFAKIYVN----------------------GDEAHPLWQFLKQRQG 172
D.erecta+3 MVCHLRDSKADIGEVFAKVRLLKLVVGCSRPRFNNLRFLQVDVNGDNAAPLYKYLKAKQT 144
D.ananaessis+3 MVCHLRDSKADIGEVFAKVN----NVYSSRS-LSHFLIFQVDVNGDNAAPLYKYLKAKQT 194
D.pseudoobscura-3 MVCHLRDAKADIGEIFARVR------------------------------LLPQLRGLFN 129
D.virilis+2 MVCHLRDSKADIGELFAKVYE-----------------------------LFIQLKHI-- 157
D.simulans+2 MVCHLRDSKADIGEVFAKVRH--------------------------------------- 112
Drosophila MVCHLRDSKADIGEVFAKVDVN----------------------GDNAAPLYKYLKAKQT 155
D.Mojavensis-2 GGHGLSSS---------------------------------------------------- 134
D.ananaessis+1 ------------------------------------------------------------
Glossina MVCHLRDAKADIGDVFQKVDVN----------------------GANAAPLYQYLKAKQG 152
Anopheles MVCHLRSAKAEVGDVFAKIDVN----------------------GDGAHPLYKYLKHKQG 158
D.yakuba-1 MLDHLRREGANIGQLFAKIDVK----------------------GAQADPLYKLLTRHQH 186
D.erecta+1 MLDHLRREGANIGHIFAKVDVK----------------------GAQADPLYKLLTRHQQ 154
D.simulans-2 MLDHLRREGANIGHLFAKIDVK----------------------GAQADPLYKLLTRHQH 154
D.ananaessis-1 ILEHLRQKKANIGHIFAKIKVN----------------------GRNADPLYKLLTRKAP 157
D.pseudoobscura-2 MMDHLRQEKANIGDVFAKIEVN----------------------GPGAAPLYKLLTRNRS 193
Ixodes IKEFREAVRRHLRHVQQDQRER-----------------------GQRPPALEVPEGEAA 159
GPx4 GKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPLVIEKDLPHYF----- 197
Apis G--FITDDIKWNFSKFIINKEGKVVSRFAPTVDPLQMESELKKYF----- 217
Aedes G--TLFDAIKWNFTKFIVDKNGQPVERHGPQTSPLQLRDNLKKYFAQ--- 217
D.erecta+3 G--TLGSGIKWNFTKFLVNKEGVPINRYAPTTDPMDISKDIEKLL----- 187
D.ananaessis+3 G--TLGSGIKWNFTKFLVNKEGIPVNRYAPTTDPMDIAKDIEKLL----- 237
D.pseudoobscura-3 D--RSSC------------------------------------------- 134
D.virilis+2 --------------------------------------------------
D.simulans+2 --------------------------------------------------
Drosophila G--TLGSGIKWNFTKFLVNKEGVPINRYAPTTDPMDIAKDIEKLL----- 198
D.Mojavensis-2 --------------------------------------------------
D.ananaessis+1 --------------------------------------------------
Glossina G--TLVSAIKWNFTKFLVNKEGIPVKRYAPTTDPMDIAKDIEKLL----- 195
Anopheles G--TLGDSIKWNFAKFLVNKDGQPVDRYAPTTSPSSIVKDIDKLLG---- 202
D.yakuba-1 -------DIEWNFVKFLVDRRGNIYKRYGAELEPVALTDDIELLLGR--- 226
D.erecta+1 -------DIEWNFVKFLVDRKGNIHKRYGAELEPVALTDDIELLLGR--- 194
D.simulans-2 -------DIEWNFVKFLVDRKGNIHKRYGAELEPVALTDDIELLLGR--- 194
D.ananaessis-1 -------RIEWNFVKFLIDRKGNIYGRYGAEKKPAVLVNDIERLLLEA-- 198
D.pseudoobscura-2 N--LRGGKIEWNFVKFLVDRRGHVYARYGAQAEPSALSGDIERLLGEGSK 241
Ixodes G--LPLQRHQMELHQVSGGQERTAREALWTHRLVRNNRSRPSQVLLEE-- 205